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http://nopr.niscpr.res.in/handle/123456789/60024| Title: | Antiproliferative potential of Sargassum binderi Sonder ex J. Agardh mediated biogenic silver/silver chloride nanoparticles on EAC, MCF-7 and HCT116 cells |
| Authors: | Ahamed, M S Rahman, M M Hasan, I Kabir, S R Asaduzzaman, A K M |
| Keywords: | Ag/AgCl-NPs;Apoptosis;Cancer cell inhibition;Gene expression;Sargassum binderi |
| Issue Date: | Feb-2022 |
| Publisher: | NIScPR-CSIR, India |
| Abstract: | Biosynthesis of Ag/AgCl-NPs from Sargassum binderi extract was confirmed by colour changes and a sharp peak at 416 nm detected by UV-visible spectrophotometer. It was characterized by Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX) spectrophotometer, X-ray powder Diffraction (XRD) and Fourier-Transform Infrared Spectroscopy (FTIR). Mild toxicity of the synthesized Ag/AgCl-NPs was determined by the brine shrimp nauplii cytotoxicity assay. IC50 values of 19.73, 25.56, and 14.00 𝜇g/ml showed the growth inhibition of Ehrlich Ascites Carcinoma (EAC), human breast cancer (MCF-7) and human colon cancer (HCT116) cells by Ag/AgCl-NPs, respectively. By analyzing cell morphological study using annexin V and caspase-3 inhibitor, initiation of apoptosis in EAC cells was confirmed. The results were further supported through the expression of several genes, where, p53 and Bax genes became activated and Bcl-X and NFκB genes were suppressed. |
| Page(s): | 126-137 |
| ISSN: | 2582-6727 (Online); 2582-6506 (Print) |
| Appears in Collections: | IJMS Vol.51(02) [February 2022] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJMS 51(2) 126-137.pdf | 1.45 MB | Adobe PDF | View/Open |
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