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Callus induction and plant regeneration from leaf explants of jojoba [Simmondsia chinensis (Link) Schneider]

2014-01-30T07:13:24Z

Title: Callus induction and plant regeneration from leaf explants of jojoba [Simmondsia chinensis (Link) Schneider] Authors: Kumar, Sunil; Mangal, Manisha; Dhawan, A K; Singh, Narender Abstract: An efficient micropropagation protocol involving callus induction and shoot regeneration has been standardized in Simmondsia chinensis, an oil yielding, medicinal and multi-purpose plant species. Higher percent of callus proliferation (97.3%) was obtained from leaf explants, taken from field grown mature plant, when cultured on MS medium supplemented with 2,4-D (2.0 mg L^-1)+BAP (0.5 mg L^-1)+CH (100 mg L^-1) within 20-22 d of inoculation. The callus was yellowish green in colour and soft in texture. Further, optimum shoot regeneration was obtained from the leaf derived callus on MS medium fortified with BAP (2.0 mg L^-1)+NAA (0.5 mg L^-1)+GA₃ (0.3 mg L^-1). About 92% cultures responded with an average number of 9.1 shoots per culture. The shoots obtained via callogenesis were rooted on half-strength agar-solidified MS medium supplemented with IBA (1.0 or 2.5 mg L^-1). The medium containing 2.5 mg L^-1 IBA was the best for rooting of shoots. The rooted shoots were transplanted to soil with 75% success. The protocol will be of immense importance in rapid mass multiplication of elite germplasm, as well as for conservation of this important species. Page(s): 544-547

Algal Database—Bioprospecting indigenous algae for industrial application

2014-01-28T16:38:20Z

Title: Algal Database—Bioprospecting indigenous algae for industrial application Authors: Sharma, P; Patil, P; Rao, N; Swamy, K V; Khetmalas, M B; Tandon, G D Page(s): 548-549

Molecular analysis for genetic structure of biotic and abiotic stress resistant genotypes in chickpea (Cicer arietinum L.)

2014-01-30T07:11:51Z

Title: Molecular analysis for genetic structure of biotic and abiotic stress resistant genotypes in chickpea (Cicer arietinum L.) Authors: Singh, Reema; Kumari, Nilima; Upadhyaya, Hari D; Yadav, Renu; Vaishali; Choi, Insoo; Kumar, Rajendra Abstract: Molecular characterization of biotic (Ascochyta blight, Fusarium wilt & dry root rot) and abiotic (drought & salinity) resistant genotypes of chickpea was carried out using a specific set of 20 polymorphic STMS (sequence tagged microsatellite site) markers. The number of alleles ranged from 1 to 3 alleles per locus. The PIC (polymorphism information content) value ranged from 0.0 to 0.656 with an average of 0.268, indicating the considerable efficiency of markers for studying the polymorphism level. The primer GA-33 showed maximum PIC value (0.656), while the ten primers had the 0.0 value. The dendogram derived from the analysis had six clusters. Many pairs showed the maximum similarity (0.983), whereas accessions ICC-1392 and ICC-2065 showed the minimum similarity (0.900). The conservation of genotypes ICC-2580, ICC-1392 and ICC-2065 was recommended for their utilization in Middle-east Asia region chickpea improvement programme. Page(s): 537-540

Fermentation variables for the fermentation of glucose and xylose using Saccharomyces cerevisiae Y-2034 and Pachysolan tannophilus Y-2460

2014-01-31T16:36:12Z

Title: Fermentation variables for the fermentation of glucose and xylose using Saccharomyces cerevisiae Y-2034 and Pachysolan tannophilus Y-2460 Authors: Kocher, G S; Uppal, Shivali Abstract: The fermentation variables (temperature, pH and agitation) were optimized by response surface methodology (RSM) alogorithm, Design Expert 7.1 and a response quadratic model was generated that revealed a correlation between all these parameters and also provided 23 solutions for process validation. Under the optimized conditions, the effect of inoculum size revealed 5.0 and 2.5% (v/v) of Saccharomyces cerevisiae Y-2034 and Pachysolan tannophilus Y-2460, respectively as optimum for sequential fermentation. The optimization of sequential fermentation led to improvement in total ethanol yield from 20.61 to 22.24 g L^-1. Page(s): 531-536