NOPR Collection:

Production of Plumbagin (5-hydroxy-2-methyl-1, 4-naphthoquinone) in Callus and Cell Suspension Cultures of Plumbago indica Linn.

2013-07-19T16:34:02Z

Title: Production of Plumbagin (5-hydroxy-2-methyl-1, 4-naphthoquinone) in Callus and Cell Suspension Cultures of Plumbago indica Linn. Authors: Satheeshkumar, K; Seeni, S Abstract: The relationship between growth and plumbagin (5-hydroxy-2-methyl-l, 4-naphthoquinone) formation in callus and cell suspension cultures of Plumbago indica was investigated. Semi-friable callus was induced on young stem and leaf segments in MS solid medium containing 1.5-2.5 mgl^-1 2, 4-D plus 0.5-1.5 mgl^-1KN. Cell suspension cultures were established from stem-derived semi-friable calli and cells released from aseptic leaf segments. Quantitative analysis of plumbagin in callus and cell cultures at different periods of growth revealed an increase in concentration of plumbagin with the increase in growth of cultures. Plumbagin level in both culture systems on unit dry wt basis was same throughout the growth of callus (0.05 mg/g, dry wt) and cell cultures (0.028 mg/g, dry wt). Page(s): 305-308

In Vitro Regeneration and Hydroxycitric Acid Production in Tissue Cultures of Garcinia indica Chois

2013-07-25T16:33:23Z

Title: In Vitro Regeneration and Hydroxycitric Acid Production in Tissue Cultures of Garcinia indica Chois Authors: Kulkarni, Manik D; Deodhar, Manjushri A Abstract: Garcinia indica is a rich source of hydroxycitric acid (HCA), an important biological active plant metabolite used as an anti-obesity drug. The female plants are the major source of main isomer, (-) - hydroxycitric acid, a powerful inhibitor of citrate cleavage enzyme. G. indica being a polygamodioecious sp, differentiation between male and female plants is detected only at the flowering stage (approx. 7 yrs). Tissue culture methods, offer scope for large scale propagation of female plants. An efficient protocol for multiple shoots and callus has been standardized to produce HCA. Multiple shoots were obtained from immature seed explants on MS basal medium supplemented with NAA (2.69 μM), BAP (8.9 μM) and KN (0.93 μM). Elongation of shoots was achieved on half MS medium supplemented with NAA (0.54 μM), BAP (0.44 μM) and KN (0.93 μM). The shoots developed roots when they were treated with 4900 μM IBA for 30 seconds and cultured on half MS basal medium. Multiple shoots and callus were obtained from explants derived from in vitro developed plantlets. The regenerated plantlets were successfully transferred to soil in pots. Multiple shoots and the callus cultures produced HCA. Page(s): 301-304

Agrobacterium-mediated Transformation of Lucerne (Medicago sativa Linn.): Optimizing Biological and Physical Parameters

2013-07-20T16:33:34Z

Title: Agrobacterium-mediated Transformation of Lucerne (Medicago sativa Linn.): Optimizing Biological and Physical Parameters Authors: Kumar, Suresh; Bhat, Vishnu; Bhat, B V; Gupta, M G Abstract: An optimized protocol for the transformation of Indian cultivars of lucerne (Medicago sativa Linn.) using Agrobacterium tumefaciens has been developed. It involved a selectable marker gene encoding hygromycin phosphotransferase (hpt II) which confers resistance to the antibiotic hygromycin, together with a reporter gene (uid A) encoding P-D glucuronidase (GUS); cotyledonary and hypocotyl explants from in vitro grown 5 days-old seedlings raised from mature seeds of genotype L-2 and IL-75 as target tissues, and co-cultivation of explants with A. tumefaciens strain LBA-4404 harbouring plasmid pCAMBIA-1301. Cotyledonary explant was found to be more responsive for transformation. Bacterial density of 5×10⁹ cells/ml and co-cultivation for three days were found to be most effective for stable GUS expression in 91 % of the hygromycin-resistant calli. Page(s): 298-300

Comparison of ELISA and GC Methods to Detect DDT Residues in Water Samples

2013-07-23T16:32:59Z

Title: Comparison of ELISA and GC Methods to Detect DDT Residues in Water Samples Authors: Amitarani, B E; Pasha, Akmal; Gowda, Putte; Nagendraprasad, T R; Karanth, N G K Abstract: ELISA and GC methods were used to analyse DDT residues in about 30 water samples collected from different talukas of Mandya District of Karnataka. Polyclonal antibody based immunoassay developed at CFTRI, Mysore, performed well to detect the DDT residues. The minimum detectable level of DDT by ELISA was one part per billion (ppb) in the water samples tested. The insecticide residue ranged from 1 to 20 ppb. Experiments also revealed no matrix effect and hence did not require any prior clean-up. The pH of the water did not interfere in the assay. The ELISA method validated in the present work is specific to DDT. The results of ELISA with respect to DDT residues were found to be comparable to values obtained from the GC analysis of the water samples. The water samples could be directly used for ELISA test, thereby making the analysis quick, simple and cost effective. Page(s): 292-297