NOPR Collection:

Effect of nifedipine and amlodipine on dead space wound healing in rats

2013-11-09T16:36:10Z

Title: Effect of nifedipine and amlodipine on dead space wound healing in rats Authors: Bhaskar, H N; Udupa, Saraswathi L; Udupa, A L Abstract: Effect of two calcium channel blockers (CCBs) nifedipine and amlodipine, was studied on normal and steroid depressed wound healing in albino rats, using the dead space wound model. The drugs enhanced normal healing as evidenced by increase in tensile strength of 10 days old granulation tissue. There was neither a significant change in the hydroxyproline level (or collagen) nor a change in the glycosaminoglycan content in granulation tissue. However, lysyloxiclase level was increased significantly. The increase in tensile strength could thus be attributed to better cross-linking and maturation of collagen rather than collagen synthesis per se. The drugs were also able to overcome steroid depressed wound healing. It is likely that the prohealing effects may be related to the improved antioxidant status too, since superoxide dismutase levels were observed to be higher in the CCB-treated animals. Page(s): 294-296

In vivo induction of sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) by lynestrenol

2013-11-10T16:35:42Z

Title: In vivo induction of sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) by lynestrenol Authors: Siddique, Yasir Hasan; Afzal, Mohammad Abstract: Genotoxicity study of synthetic progestin lynestrenol, was carried out on mouse bone marrow cells using sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) as parameters. Lynestrenol was studied at three different doses (6.87, 13.75 and 27.50 mg/kg body wt.). SCE and CA increased significantly as compared to normal control when treated with lynestrenol at 13.75 and 27.50 mg/kg body wt. The present results suggest that lynestrenol has both a genotoxic and cytotoxic effects in mouse bone marrow cells. Page(s): 291-293

Inter-specific hybridization between freshwater catfish Mystus cavasius (Ham & Buch) and M. seenghala (Sykes) by artificial fertilization

2013-11-10T16:35:24Z

Title: Inter-specific hybridization between freshwater catfish Mystus cavasius (Ham & Buch) and M. seenghala (Sykes) by artificial fertilization Authors: Raj, A Jesu Arockia; Haniffa, M A; Seetharaman, S; Benziger, P S Allen; Jacob, Shybu Abstract: By employing the technique of induced ovulation for artificial fertilization, inter-specific hybrids between the threatened catfish species (Mystus cavasius × M. seenghala) were produced. Fertilization, hatching and survival were significantly different between control and hybrids. The hatching time of the hybrid was significantly lower than that of the control. The average performance viz., hatching time and viability of larvae of the control fish, was significantly better than that of the hybrids. In the hybrid cross, hatchlings were mostly deformed and abnormal and after yolk absorption ultimately succumbed. Page(s): 286-290

In vitro propagation of Dendrobium hybrids using flower stalk node explants

2013-11-07T16:36:54Z

Title: In vitro propagation of Dendrobium hybrids using flower stalk node explants Authors: Martin, K P; Geevarghese, Julie; Joseph, Dominic; Madassery, Joseph Abstract: Large-scale in vitro propagation protocol for Dendrobium hybrids Sonia 17 and 28, two highly prized commercial cut flower cultivars through shoot multiplication using flower stalk node explants and protocorm-like bodies (PLBs) formation was accomplished. Both hybrids did not exhibit significant differences in initiation, multiplication, rooting, and field establishment. Flower stalk nodes cultured on half strength Murashige and Skoog (MS) medium supplemented with 6.97 μM kinetin (Kn), or 15% coconut water (CW) or 13.3 μM of N⁶-benzyladenine (BA) evoked bud break. Kn showed better growth of the initiated bud. Excision and culture of the initiated shoots on medium having same amount of Kn developed more than 5 shoots per shoot directly from the base. Subsequent culture enhanced the rate of shoot induction. Transfer of isolated shoots onto 44.4μM of BA enriched medium displayed induction of more than 6 PLBs from the base within 60 days. PLBs underwent rapid multiplication upon transferral to medium having the same concentration of BA (44.4 μM). Subsequent culture increased the proliferation of PLBs. No decline was observed in the proliferation of shoots as well as PLBs up to 15^th subculture. PLBs transferred onto half strength MS medium with 6.97 μM of Kn underwent conversion of more than 90% PLBs to shoots. The shoots were rooted at the best on half strength MS medium with 2 g 1^-1 activated charcoal. Survival rate of the plantlets of the two hybrid cultivars after acclimatization was more than 80%. Page(s): 280-285