NOPR Collection:

Effect of Mesua ferrea Linn. flower extract on Salmonella

2016-07-20T05:20:37Z

Title: Effect of Mesua ferrea Linn. flower extract on Salmonella Authors: Mazumder, R; Dastidar, Sujata G; Basu, S P; Mazumder, Avijit Abstract: Based on its traditional uses in folk medicine, the whole flower extract of Mesua ferrea Linn. was tested for its in vitro antimicrobial efficacy against five different strains of Salmonella spp. All the strains were found to be highly sensitive to the extract. MIC of the extract against each organism being 50 μg/ml. The extract was tested in vitro for its mode of antibacterial activity against S. Typhimurium NCTC 74 and it was found to be bactericidal in action. In vivo studies of this extract offered significant protection to Swiss albino mice at doses ≈ 2 and 4 mg/mouse when challenged with 50 median lethal dose of S. Typhimurium NCTC 74. Further, the ex tract caused statistically significant reduction in viable count of the strain in liver, spleen and heart blood of challenged mice. Page(s): 566-568

In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds

2016-07-20T05:20:25Z

Title: In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds Authors: Kaushal, N; Modgil, M; Thakur, M; Sharma, D R Abstract: In vitro clonal multiplication of apple rootstock M M 111 using axillary buds and shoot apices were carried out. Vegetative axillary buds of the size of 0.2-2.0 cm and shoot apices measuring 4 mm in length were initiated to shoot proliferation on MS medium supplemented with BA (0.5-1.0 mgl^-1), GA₃(0.5 mgl^-1), with or without IBA (0.05-0.1 mgl^-1). Small size ex plants showed less phenol exudation and less contamination. Following establishment phase, the small shoots emerged from explants were subcultured on M S medium supplemented with different combinations and concentrations of growth regulators. B A (1.0 mgl^-1) and GA₃(0.5 mgl^-1) combination showed highest multiplication rate (1:5) , and also produced longer shoots. Two step rooting was done by transferring microcuttings to auxin free solid medium after root initiation in dark on 1/2 strength M S liquid medium containing IBA (0.5 GA₃(0.5 mgl^-1). Rooted plantlets were transferred to peat containing paper cups and resulting plants of MM 111 acclimated successfully for transfer to field. Page(s): 561-565

Biochemical studies of embryogenic and non-embryogenic callus of Cardiospermum halicacabum L.

2016-07-20T04:59:38Z

Title: Biochemical studies of embryogenic and non-embryogenic callus of Cardiospermum halicacabum L. Authors: Jeyaseelan, M; Rao, M V Abstract: Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nucleic acids, phenols and ascorbic acid. Non-embryogenic callus exhibited high chlorophyll content, total soluble sugar, protein, ammonia and enzymes like peroxidase and polyphenol oxidase. Thus, the present study indicated that the process of somatic embryogenesis was characterized by some biochemical and physiological changes induced by plant growth regulators. Page(s): 555-560

In vitro response of encapsulated somatic embryos of Lagerstroemia indica L.

2016-07-20T05:19:31Z

Title: In vitro response of encapsulated somatic embryos of Lagerstroemia indica L. Authors: Niranjan, M H; Sudarshana, M S Abstract: A method to produce encapsulatable units for synthetic seeds was developed in L. indica. Somatic embryos were harvested from leaf derived embryogenic callus on Murashige and Skoog's basal medium supplemented with 2,4-dichlorophenoxy acetic acid (2, 4-D, 0.5 mg/l), 6-benzyl amino purine (BAP, 1 mg/l) and ascorbic acid (AA, 50 mg/l) . The embryos were encapsulated in alginate beads and dehydrated. Germination ability of the artificial seeds were investigated. The frequency of regeneration from the encapsulated embryos was significantly affected by (i) the concentration of alginate (ii) the duration of storage, and (iii) the effect of different types of media. A 2% sodium alginate concentration on MS salts resulted in significantly higher germination frequencies than at other concentrations. L. indica showed maximum germination on MS medium (93.84%) after 6 weeks of culture. The germinated synthetic seeds with well developed roots and shoots were transferred successfully to green house. This is the first report on artificial seeds in Lagerstroemia indica. Page(s): 552-554