NOPR Collection:

High frequency shoot regeneration from nodal and shoot tip explants in Holarrhena antidysenterica L.

2013-11-24T16:39:17Z

Title: High frequency shoot regeneration from nodal and shoot tip explants in Holarrhena antidysenterica L. Authors: Ahmed, Golam; Roy, P K; Mamun, AN K Abstract: Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/1) with NAA (0.2-1.0 mg/1) and BAP (1.0-3.0 mg/1) with Kn. (0.2-1.0 mg/1) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/1) and NAA (0.5 mg/1). Subculture on the same medium resulted in rapid shoot multiplication at an average rate of 16 new shoots per subculture. Addition of urea (100 mg/1) in the medium increased the number of shoots up to 22 per culture. For best rooting, the shoots were excised from the culture flask and implanted individually on half strength MS medium with 0.5 mg/1 each of IBA, IAA and NAA. After 20 days of transfer on root induction medium 95% rooting was achieved. Regenerated plantlets were successfully acclimatized and established in soil. About 90% of plantlets survived under open field conditions. Page(s): 1322-1324

Effect of metal ions on growth of Pseudomonas aeruginosa and siderophore and protein production

2013-11-23T16:41:33Z

Title: Effect of metal ions on growth of Pseudomonas aeruginosa and siderophore and protein production Authors: Gupta, C P; Sharma, A; Dubey, R C; Maheshwari, D K Abstract: Pseudomonas aeruginosa (GRC₁ ) isolated from potato rhizosphere, grew better on succinate medium than tryptic soy medium and produced hydroxamate type of siderophore in iron-deficient succinate medium. When the strain GRC₁ was grown in the presence of different metal ion compounds, viz. ZnSO₄, MnSO₄, MnCl₂ and FeCl₃ at 6 and 12 μM concentrations individually, ZnSO₄ (12 μM) promoted siderophore production but suppressed the growth and protein content of test organism. MnCl₂ and FeCl₃ (12μM) enhanced the growth, whereas MnCl₂ and MnSO₄ (12 μM) induced protein contents of strain GRC₁. Page(s): 1318-1321

Virus enhancement following infection with antibody-coated infectious bursal disease virus (IBDV) in chickens

2013-11-23T16:41:29Z

Title: Virus enhancement following infection with antibody-coated infectious bursal disease virus (IBDV) in chickens Authors: Kumar, Rajesh; Charan, Shiv Abstract: A novel concept of vaccination, employing virus-antibody complex has been reported for the control of infectious bursal disease in chickens. A comparison of virus replication, serum neutralizing antibody response and pathogenicity in chickens inoculated with the antibody coated virus, prepared by mixing virus and antibody in different ratios (1:1, 1:0.1, 1: 0.01) and virus alone without antibody, has been made. Antibody coated virus (when mixed in certain crucial ratios) replicated to a higher magnitude in the target organ, caused more severe pathogenesis but induced a primary serum neutralizing antibody response almost comparable. The results may have important implications in understanding of pathogenesis and development of control strategies against infectious bursal disease virus, specially employing immune complex vaccine. Page(s): 1314-1317

Edwardsiella tarda endotoxin as an immunopotentiator in singhi, Heteropneustes fossilis fingerlings

2013-11-27T16:42:55Z

Title: Edwardsiella tarda endotoxin as an immunopotentiator in singhi, Heteropneustes fossilis fingerlings Authors: Das, B K; Pattnaik, P; Murjani, G; Mukherjee, S C Abstract: Endotoxin of E. tarda grown in brain heart in fusion broth at 30°C for 18 hr was extracted by differential centrifugation. Fingerlings of H. fossilis (weighing 1-2 g) were allowed for hyperosmotic infiltration in the endotoxin at the rate of 0,2,4,8, 16 and 20 mg/1. Mortality varied from 20-50% at 2 to 20 mg/ml. Toxin treated fishes were challenged 21 days post treatment with the same E. tarda strain containing 2.1× 10⁹ CFU/ml. There was 80% mortality in the control group whereas only 20% mortality in toxin treated group at 2 mg/1 concentration after challenge with homologous E. tarda. Subsequently a second challenge of E. tarda was given to the survivors of fish one month after first challenge using same concentration where no mortality could be observed. It was concluded that the endotoxin could enhance percentage of survival against E. tarda infection in Singhi. Page(s): 1311-1313