NOPR Collection:

Micropropagation of Sapindus mukorossi Gaertn

2013-11-25T16:37:20Z

Title: Micropropagation of Sapindus mukorossi Gaertn Authors: Philomina, N S; Rao, J V S Abstract: Bud break and multiple shoots were induced in apical and axillary meristems derived from one month old seedlings of S. mukorossi on Murashige and Skoog (MS) medium supplemented with benzylamino purine (BAP) 0.4 μM or 0.8 μM alone. A combination of BAP and gibberellic acid (GA₃) 0.4 μM and 2.8 μM produced elongated multiple shoots from both types of explants. Excised shoots were rooted on MS medium respectively with indole-3-butyric acid (IBA) 3.4 μM or 2.4 μM. The regenerated plantlets were successfully acclimatized and transferred to soil. Page(s): 621-624

Nitrosomethylurea induced streptomycin resistance in Lycopersicon esculentum Mill.

2013-11-22T16:37:33Z

Title: Nitrosomethylurea induced streptomycin resistance in Lycopersicon esculentum Mill. Authors: Rao, A V; JayaSree, T; Ramesh, M; Pavan, U; Sadanandam, A Abstract: High frequency of streptomycin resistant variants of Lycopersicon esculentum were isolated on selective shoot regeneration medium supplemented with IAA (0.5 mg/L), zeatin (1.5 mg/L) and streptomycin sulphate (500 mg/L). Nonmutagenized (controls) and NMU treated cotyledons were placed on shoot regeneration medium supplemented with antibiotic streptomycin. Resistant shoots appeared at a high frequency in mutagenized cotyledons, whereas in controls morphogenesis was suppressed, accompanied by bleaching. Shoot regeneration occurred from the nodular tissues developed at the cut ends of cotyledons. Resistant shoots developed into complete plantlets on rooting medium containing selective concentration of antibiotic. Stability of streptomycin resistance was confirmed by leaf assay and reciprocal crosses between streptomycin-resistant and sensitive plants. Page(s): 617-620

Role of satellite RNA of an Indian isolate of cucumber mosaic virus in inducing lethal necrosis of tobacco plants

2013-11-21T16:38:09Z

Title: Role of satellite RNA of an Indian isolate of cucumber mosaic virus in inducing lethal necrosis of tobacco plants Authors: Raj, S K; Srivastava, A; Chandra, G; Singh, B P Abstract: Lethal necrosis or systemic stem necrosis followed by death of Nicotiana benthamiana, severe leaf deformations of N. tabacum cv. white burley and blister formations on N. tabacum cv. samsun NN symptoms were induced by experimental inoculations of CMV RNA preparations containing satellite RNA (sat-RNA). Inoculations of RNA preparations without sat-RNA did not induce that severe symptoms on these plants, only late mild mosaic was observed. It is suggested that sat-RNA of CMV isolate has a certain role for enhancing severity of symptoms in tobacco plants. Local and systemic lethal necrosis of N. benthamiana is due to sat-RNA present with genome of CMV isolate. It is the first report of lethal necrosis induced in N.benthamiana by CMV satellite. Page(s): 613-616

[TAME]-esterase — A new cardiovascular risk factor in smokers

2013-11-22T16:36:45Z

Title: [TAME]-esterase — A new cardiovascular risk factor in smokers Authors: Subratty, A H; Mooradun, A; Jowaheer, V Abstract: A mathematical model has been proposed to study the effect of [TAME] esterase on blood clotting time. Using this model, clotting time was found to decrease by 30% with increasing plasma [TAME] esterase activity in a group of smokers. It is hypothesized that [TAME] esterase through its effect on Hageman factor could affect clotting time. However mechanism of clotting by [TAME] esterase remains to be elucidated. It is concluded that [TAME] esterase is involved in the cascade of reactions leading to blood coagulation and increased [TAME] esterase activity could be an additional risk factor for possible cerebro-vascular accidents in smokers. Page(s): 610-612