NOPR Collection:

Characterization of anionic amino acid transport systems in mouse mammary gland

2013-11-27T16:46:34Z

Title: Characterization of anionic amino acid transport systems in mouse mammary gland Authors: Kansal, Vinod K; Sharma, Rekha; Rehan, Gayatri Abstract: -glutamate was transported into mammary tissue via Na⁺-dependent system X_AG- that strongly interacted with both and -isomers of aspartate but only with -isomer of glutamate. Replacement of Cl ̅ by gluconate from the extracellular medium did not affect the uptake of -glutamate. Although neutral amino acids weakly inhibited the uptake of -glutamate, there was no evidence for the heterogeneity of anionic amino acid transport system. The X_AG- system was inhibited by sulfhydryl group blocking reagent N-ethylmalemide. Low pH (6) partially inhibited the uptake by -glutamate by mammary tissue. Prior loading of mammary tissue with -glutamate slightly down regulated its uptake. Culturing pregnant mouse mammary tissue explants in vitro in the presence of lactogenic hormones (insulin plus cortisol plus prolactin) did not affect appreciably the uptake of -glutamate. Page(s): 1097-1103

Enhancing the efficacy and persistency of Spodoptera litura (Fab.) nuclear polyhedrosis virus using UV irradiation protectants

2013-11-25T16:45:19Z

Title: Enhancing the efficacy and persistency of Spodoptera litura (Fab.) nuclear polyhedrosis virus using UV irradiation protectants Authors: Arivudainambi, S; Selvanarayanan, V; Vikash, A Abstract: To enhance the field persistency of S. litura nuclear polyhedrosis virus (SLNPV), three chemicals viz. cupric ammonium nitrate, tinopal and cupric sulphate were tried as protectants (0.01 mg/ml) against natural sunlight (UV) irradiation. On exposure for 8 hr and subsequent bioassaying (diet surface treatment), it was found that cupric sulphate protected the polyhedrosis inclusion bodies (PIBs), recording 95.56 % mortality which was statistically at par with unexposed PIBs recording 97.78% mortality. Page(s): 1175-1176

Role of ATP sensitive potassium channel on 7-hydroxy flavone induced antinociception and possible association with changes in glycaemic status

2013-11-24T16:40:06Z

Title: Role of ATP sensitive potassium channel on 7-hydroxy flavone induced antinociception and possible association with changes in glycaemic status Authors: Venkataramanan, P E; Parvathavarthini, S.; Viswanathan, S; Ramaswamy, S Abstract: Opioid type of analgesics open A TP sensitive potassium channel at the cellular level to produce antinociceptive response. These channels have also been shown to modulate insulin secretion by the pancreas.7-hydroxy flavone, an antinociceptive agent shown to act through opioid pathways was investigated for its effect on glycaemic state and associated algesic state. The involvement of ATP sensitive potassium channel in the action was examined by using glybenclamide. The result reveal that 7-HF per se did not elicit any significant change in the glycaemic state simultaneously eliciting antinociceptive response as tested by acetic acid induced abdominal constriction assay procedure. Glibenclamide treatment attenuated the antinociceptive effect of 7-HF and while maintained its hypoglycaemic response. The present finding suggest that 7-HF induces antinociception like morphine, utilise A TP sensitive potassium channel at the cellular level and do not suggest a cause-effect relationship between the changes in the glycaemic and algesic state. Possibly, insulin which is controlled by ATP sensitive potassium channel at the cellular level might also modulate antinociception exhibiting a cause effect relationship between them. Page(s): 1172-1174

Biodiversity of Anabaena azollae isolates from different Azolla cultures

2013-11-23T16:46:30Z

Title: Biodiversity of Anabaena azollae isolates from different Azolla cultures Authors: Subhashini, R; Kumar, K; Kannaiyan, S Abstract: The random amplified polymorphic DNA (RAPD) profile of A. azollae strains isolated from four different Azalia cultures was studied by using different primers. The objective of this study was to determine whether polymerase chain reaction (PCR) with different primers could differentiate the isolated A. azollae strains from one another. The primers amplified specific sequences of the isolates and generated fingerprinting pattern characteristic of each isolate. Clear polymorphism was noticed among all the strains which depends on the primer sequence. Page(s): 1168-1171