NOPR Collection:

Antiangiogenic and antiproliferative assessment of cyanobacteria

2016-07-20T05:09:53Z

Title: Antiangiogenic and antiproliferative assessment of cyanobacteria Authors: Kyadari, Mahender; Fatma, Tasneem; Velpandian, Thirumurthy; Malliga, P; Bharat, Naveen; Bano, Fareha Abstract: Biologically active compounds with different modes of action, such as antiproliferative, antioxidant, antimicrotubule, have been isolated from algae and cyanobacteria. The present study was designed to evaluate antiangiogenic and antiproliferative potential of dichloromethane and methanol (2:1) extracts of different cyanobacteria. Further fingerprinting of the activity possessing extracts were carried out using ESI-LC-MS/MS. Extracts (25, 50 and 100 µg) were screened in the vascular endothelial growth factor (VEGF) induced angiogenesis in inovo chick chorioallontoic membrane assay (CAM) at various concentrations using thalidomide and normal saline as positive and untreated control groups respectively. The extracts were also evaluated for their antiproliferative activity by MTT assay using HeLa cancer cell line. The results obtained from the various algal extracts did not show any significant antiangiogenic activity as compared to VEGF control. Oscillatoria sp. and Lyngbya officinalis exhibited significant anti-proliferative activity at IC₅₀ values of 220 and 260 µg/mL respectively. ESI-LC-MS/MS of L. officinalis showed the presence of lyngbyatoxin-A and that of Oscillatoria sp. reveals the presence of malyngamide-J suggesting the possibility of antiproliferative activity. Page(s): 835-842

In vitro flowering – A system for tracking floral organ development in Dendrocalamus hamiltonii Nees et Arn. ex Munro^†

2016-07-20T05:09:00Z

Title: In vitro flowering – A system for tracking floral organ development in Dendrocalamus hamiltonii Nees et Arn. ex Munro^† Authors: Kaur, Devinder; Thapa, Pooja; Sharma, Madhu; Bhattacharya, Amita; Sood, Anil Abstract: Dendrocalamus hamiltonii plants are slender and tall (15-25 m) thereby, rendering tagging, sampling and tracking the development of flowers difficult. Therefore, a reproducible system of in vitro flowering was established for tracking the stages of flower development. MS medium supplemented with 2.22 µM6-benzylaminopurine, 1.23 µMindole-3-butyric acid and 2% sucrose was optimized as the flower induction medium (FIM) wherein 28 and 42 days were required for the development of gynoecium and androecium, respectively. Six distinct stages of in vitro flower development were identified, and the flowers were comparable with that of in planta sporadic flowers. Pollen viability of the in vitro flowers was higher than those of in planta ones. The in vitro system developed in the present study facilitates easy tracking of different stages of flower development under controlled environmental conditions. It can also be used for medium- or long-term storage of pollens and manipulation of in vitro fertilization. Page(s): 825-834

A simple technique for tracking individual spore and gametophyte development in Adiantum lunulatum Burm. f. using modified extra thin alginate film technique^†

2016-07-20T05:07:59Z

Title: A simple technique for tracking individual spore and gametophyte development in Adiantum lunulatum Burm. f. using modified extra thin alginate film technique^† Authors: Sareen, Bhuvnesh; Bhattacharya, Amita; Sharma, Madhu; Sood, Anil; Ahuja, Paramvir Singh Abstract: A new technique was developed for accurate calculation of percent germination and tracking of individual spores from germination to gametophyte development in Adiantum lunulatum. High percentage of ETAF immobilized spore germination (72.4%) was followed by development of gametophytic clumps. The ETAF immobilized clumps were cut into pieces and multiplied en masse. Apomictic sporophytes developed from the gametophytes. This indicated the potential of ETAF for mass propagation of A. lunulatum without the need to start from spores. Since individual spores can be tracked from germination to gametophyte development, the ETAF technique has the potential to be used for (i) harvesting uniformly developed plants of similar age for extensive experimentations and commercial utilization and (ii) detailed study on developmental and reproductive biology of different ferns and fern allies. Page(s): 820-824

Effect of acute exposure of triazophos on oxidative stress and histopathological alterations in liver, kidney and brain of Wistar rats

2016-07-20T05:06:58Z

Title: Effect of acute exposure of triazophos on oxidative stress and histopathological alterations in liver, kidney and brain of Wistar rats Authors: Mohineesh; Raj, Jaya; Rajvanshi, A C; Dogra, T D; Raina, Anupuma Abstract: Acute dose of organophosphorus pesticide Triazophos (O,O-diethyl O-1-phenyl-1H-1,2,4-triazol-3-yl phosphorothioate; Tz) administered orally affects oxidative stress parameters and the histo-architecture of liver, kidney and brain tissues. The results indicate a dose dependent induction of oxidative stress as evident by increased malondialdehyde level and decreased antioxidant defense including glutathione and superoxide dismutase activity in rat liver, kidney and brain. AChE activity was found significantly decreased in the Tz treated groups as compared to the vehicle control (DMSO) group. Histopathological examination of liver, kidney and brain in Tz treated rats revealed medullary congestion and hydropic degeneration of hepatocytes in liver and medullary congestion in kidney. However, no significant histopathological changes were observed in brain tissues. Page(s): 814-819

NOPR Collection:

Antiangiogenic and antiproliferative assessment of cyanobacteria

In vitro flowering – A system for tracking floral organ development in Dendrocalamus hamiltonii Nees et Arn. ex Munro†

A simple technique for tracking individual spore and gametophyte development in Adiantum lunulatum Burm. f. using modified extra thin alginate film technique†

Effect of acute exposure of triazophos on oxidative stress and histopathological alterations in liver, kidney and brain of Wistar rats

In vitro flowering – A system for tracking floral organ development in Dendrocalamus hamiltonii Nees et Arn. ex Munro^†

A simple technique for tracking individual spore and gametophyte development in Adiantum lunulatum Burm. f. using modified extra thin alginate film technique^†