NOPR Collection:

Strain improvement by induction of mutagenesis for hyper production of pectinase using Aspergillustamarii

2016-07-20T06:11:14Z

Title: Strain improvement by induction of mutagenesis for hyper production of pectinase using Aspergillustamarii Authors: Akbar, S; Prasuna, R G; Khanam, R Abstract: The present study describes the improvement of previously isolated fungal strain, Aspergillustamarii, for pectinase production by application of multistep mutagenesis using physical (UV irradiation)and chemical agents(Colchicine, Ethidium bromide and Hydrogen peroxide).Mutants obtained after 1^st treatment was screened for activity and the high producing strains were selected. The selected 1^st generation mutants were treated for two times at same concentration with same mutagen to obtain third generation mutants. The wild/parent strain cultivated in previously determined production media, Citrus peel - groundnut oil cake (CG) showed its maximum activity of pectinase production (59 U/ml). The best mutant identified in third generation mutants after quantitative analysis was H9 having treatment with Hydrogen peroxideat (concentration,9 µl). This mutant showed 8% increase in pectinase production compared to wild strain when cultivated in the CG production media.Stability of mutant was attained due to repeated mutagenic treatments. Page(s): 160-164

Correct packaging retained phytochemical, antioxidant properties and increases shelf life of minimally processed pomegranate (Punica granatum L.) arils Cv. Mridula

2016-07-20T06:07:09Z

Title: Correct packaging retained phytochemical, antioxidant properties and increases shelf life of minimally processed pomegranate (Punica granatum L.) arils Cv. Mridula Authors: Bhatia, K; Asrey, R; Varghese, E Abstract: This study investigated the effect of packaging materials (Poly propylene, low density poly ethylene and KPA bags) with different water and gas transmission rate on minimally processed ‘Mridula’ pomegranate arils stored at 5 ± 2 ºC and 85 ± 5 % RH for 15 days. During course of storage, phytochemical and antioxidant properties of minimally processed arils were determined at 3 days interval. The results indicated that packaging material influenced ascorbic acid, phenols, anthocyanins, antioxidant and sensory score of minimally processed arils. Arils packed in PP bags retained better total phenols, anthocyanins and antioxidant compared to LDPE and KPA packed arils. PP packed arils maintained higher acceptance score over the recommended commercial acceptance level (above 6.0) up to the 15 days of storage. Page(s): 141-144

Quality assessment of chromatophores isolated from squid skin as natural pigment in formulation of lipstick

2016-07-20T06:05:13Z

Title: Quality assessment of chromatophores isolated from squid skin as natural pigment in formulation of lipstick Authors: Hassan, F; Muhamed, P A; Geethalakshmi, V; Sankar, T V Abstract: Lipsticks are made to appeal to the current fashion trend and come in a wide range of colors. Lipstick is the only cosmetic ingested, and because of this strict controls on ingredients, as well as the manufacturing processes, are imposed. As long as cosmetics remain in fashion, the market for lipstick will continue to be strong, adding markets in other countries as well as diversifying currently identified markets. But the pigments used in lipsticks can cause health hazards like infertility, anemia and cancer, as well as learning disabilities, mental retardation and behavioral problems, nausea, attention deficit, headaches, skin irritation, etc. The FDA has laid strict regulations regarding the use of lead as a lipstick ingredient. In the present study the chromatophores from squid skins were isolated, and used as natural pigment in lipsticks. Five shades (SQ1, SQ2, SQ3, SQ4 and SQ5) were developed using this pigment and they were screened for consumer acceptance applying hedonic scale method (0 to 9). Comparison with the instrumental reading was also done for the sensory scores. The newly formulated lipstick (SQ2) was subjected to physical, chemical and microbiological quality evaluation. The product was compared with the commercially available lipstick brand and the new product was found to have superior properties compared to the latter (p<0.01). It was also found that the new product met with the national quality standards laid down for such category of products. The new product gives a way to utilize squid skin, which is not otherwise useful. Page(s): 171-175

Proximate, mineral composition and antioxidant properties of some wild leafy vegetables

2016-07-20T06:10:15Z

Title: Proximate, mineral composition and antioxidant properties of some wild leafy vegetables Authors: Pradhan, S; Manivannan, S; Tamang, J P Abstract: We analysed five wild leafy vegetables (Amaranthus viridis, Chenopodium album, Diplazium esculentum, Nasturtium officinale and Urtica dioica) of Sikkim for proximate and mineral composition. Wild leafy vegetables (WLV) of Sikkim are found nutritionally rich in terms of calorific value, fibre, protein and low fat which altogether indicate the potentiality of the WLVs as good source of non-conventional vegetables. WLVs were also analysed for antioxidant properties, total phenolic content and vitamin C content. All five WLVs were found to exhibit moderate antioxidant activity with variability in total phenolic content and vitamin C content. It firmly establishes rich nutritional efficiency of WLVs in the local diets. Page(s): 155-159