NOPR Collection:

Enhancement in total lipids in microalgae through cultural manipulations and fatty acid profiling

2016-07-20T06:27:26Z

Title: Enhancement in total lipids in microalgae through cultural manipulations and fatty acid profiling Authors: Bagul, Samadhan; Ratha, Sachitra Kumar; Prasanna, Radha; Saha, Supradip; Dhar, Dolly Wattal Abstract: Three microalgal genera, namely, Chlamydomonas sp., Chlorococcum sp. and Chlorella sp., were selected to optimize the environmental variables and cultural conditions for enhanced lipid production. Microalgae exhibited differential response in terms of total lipids under varied conditions of temperature, light, pH, salinity and nitrogen. Chlorella sp. showed the highest lipid production in comparison to Chlamydomonas sp. and Chlorococcum sp. Fatty acid profile indicated that Chlorococcum sp. and Chlorella sp. were rich in array of fatty acids, which are suitable for biodiesel production. Page(s): 429-432

Genetic uniformity analysis of cryopreseved in vitro plantlets of Kaempferia galanga L.—An endangered medicinal species in Tropical Asia

2016-07-20T06:26:44Z

Title: Genetic uniformity analysis of cryopreseved in vitro plantlets of Kaempferia galanga L.—An endangered medicinal species in Tropical Asia Authors: Preetha, T S; Kumar, A S Hemantha; Padmesh, P; Krishnan, P N Abstract: While using the protocols for the long-term conservation, assessment of true-to-type regenerants from cryopreserved materials is important. In the present study, no significant variation in banding pattern was noticed in the RAPD profile of cryopreserved and control samples of shoot tip-derived Kaempferia galanga plants. But in the case of somatic embryo-derived samples, some variation was observed in the banding pattern (16.2% polymorphism), though the plants were phenotypically similar. Recovery of cryopreserved somatic embryos in K. galanga was preceded through a callus phase, from which secondary embryos were induced later. The RAPD profile of somatic embryo-derived samples revealed that variation was due to the occurrence of callus phase. As minor genetic variations arising in in vitro cultures without marked phenotypic changes are considered to be beneficial for diversity conservation and sustainable utilization, cryopreserved somatic embryos of K. galanga would serve as an alternative for generating and maintaining genetic diversity of this medicinal wealth. Page(s): 425-428

Molecular assessment of genetic stability using ISSR and RAPD markers in in vitro multiplied copies of commercial banana cv. Robusta

2016-07-20T06:26:10Z

Title: Molecular assessment of genetic stability using ISSR and RAPD markers in in vitro multiplied copies of commercial banana cv. Robusta Authors: Choudhary, D; Kajla, S; Poonia, A K; Brar, B; Surekha; Duhan, J S Abstract: The present study was carried out to monitor somaclonal variations of banana cv. Robusta during micropropagation using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Shoot tips were used as explants and regenerated on MS+BAP 4.0 mg/L medium. The regenerated shoots were cultured on MS medium fortified with 4.0 mg/L BAP+0.25 mg/L NAA+30 mg/L AdSO₄ for multiplication. Rooting was obtained on ½ MS medium supplemented with 2.0 mg/L NAA. Rooted shootlets were separated individually and hardened in greenhouse. The hardened plants of banana were screened for genetic stability using 46 (26 RAPD and 20 ISSR) primers. The number of scorable bands for each RAPD primer varied from 1 to 9. Twenty-six RAPD primers produced 108 distinct and scorable bands, with an average of 4.15 bands per primer and the amplification products range was from 100-1150 bps. No polymorphism was detected during the RAPD analysis of in vitro raised clones. Twenty ISSR primers produced 81 distinct and scorable bands in the range of 100-1000 bps and the number of scorable bands for each primer varied from 2 to 8 with an average of 4.05 bands per primer. A homogenous amplification profile was observed for all the micropropagated plants when compared to mother plant in both types of markers used. The results corroborate the fact that in vitro multiplication is the safest mode for production of true to type plants. Page(s): 420-424

Molecular characterization of Assam Local pig

2016-07-20T06:24:42Z

Title: Molecular characterization of Assam Local pig Authors: Zaman, G; Shekar, M Chandra; Laskar, S; Ferdoci, A M; Rank, D N Abstract: The detailed genetic diversity of Assam Local pig population was studied through microsatellite markers. Most of the studied loci were polymorphic in nature and a total of 163 alleles were observed across all the loci. The range of alleles was found to be from 2 to 11 with a mean of 6.52±2.311. The frequency distribution of microsatellite alleles in the population was from 0.0208 to 0.9091. The overall mean of observed and expected heterozygosities were 0.50±0.272 and 0.71±0.182, respectively. The polymorphism information content (PIC) was 0.67±0.184 and Shannon’s information index (I) was sufficiently high with a mean of 1.53. The within population inbreeding estimate (F_IS) value (0.273) indicated shortfall of heterozygosity in the population. The bottleneck analysis revealed that population had not undergone any recent reduction. Page(s): 416-419