NOPR Collection:

Possible mechanism of action of antiviral proteins from the leaves of Chenopodium album L.

2016-07-20T06:45:03Z

Title: Possible mechanism of action of antiviral proteins from the leaves of Chenopodium album L. Authors: Dutt, Som; Yadav, O P; Kapoor, H C; Lodha, M L Abstract: Antiviral Protiens (AVPs) named CAP-I and CAP-II purified from the leaves of Chenopodium album cv Pusa Bathura-1 indaced systemic against tobacco mosaic virus (TMV) and sunnhemp rosette virus (SRV) in both hypersenstitive as well as systemic hosts. An increased accumulation of two polypeptides (~17 kDa and ~26 kDa) was observed in untreated upper leaves of Cyamopsis tetragonoloba plants whose basal leaves were treated with CAP-I/CAP-II. Both AVPs exhibited ribosomal RNA N-glycosidase activity on 28S rRNA of tobacco leaves and also caused in vitro degradation of TMV RNA. It is suggested that the CAP-I and –II are multi-functional and may be acting at multiple levels to ensure maximum possible inhibition of viral infection. Page(s): 29-33

Biological monitoring of lead and cadmium in human hair and nail and their correlations with biopsy materials, age and exposure

2009-04-02T16:30:13Z

Title: Biological monitoring of lead and cadmium in human hair and nail and their correlations with biopsy materials, age and exposure Authors: Mehra, Rita; Juneja, Meenu Abstract: Hair and fingernails of exposed and unexposed subjects were analyzed for their lead (Pb) and cadmium (Cd) contents by atomic absorption spectrophotometer with graphite furnace and air-acetylene flame. Hair and nail Pb concentrations in occupationally exposed subjects ranged between 1.020-409.726 and 8.130-765.306 µg/g and in environmentally unexposed subjects 0.123-25.160 and 1.076-65.613 µg/g, respectively. Similarly, hair and nail Cd concentrations in occupationally exposed subjects ranged between 0.014-22.086 and 0.214-35.714 µg/g and in environmentally unexposed subjects 0.113-1.627 and 0.028-8.108 µg/g, respectively. A significant correlation was observed between Pb hair and nail concentrations in exposed subjects at P<0.05, as compared to unexposed subjects and Cd hair and nail in exposed, as well as unexposed subjects. With respect to exposure, levels of Pb in hair and nails were found to be significant in exposed subjects, compared to unexposed ones and levels of Cd were significant only in nails of exposed ones. With respect to age, no significant correlation was found between hair and nail Pb and Cd concentrations in both exposed and unexposed subjects. Page(s): 53-56

Evidence for the presence of a critical histidine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cells

2009-04-02T16:30:46Z

Title: Evidence for the presence of a critical histidine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cells Authors: Ghosh, Swapna; Ray, Manju; Ray, Subhankar Abstract: Ehrlich ascites carcinoma (EAC) cell glyceraldehyde-3-phosphate dehydrogenase (GA3PD) (EC. 1.2.1.12) was completely inactivated by diethyl pyrocarbonate (DEPC), a fairly specific reagent for histidine residues in the pH range of 6.0-7.5. The rate of inactivation was dependent on pH and followed pseudo-first order reaction kinetics. The difference spectrum of the inactivated and native enzymes showed an increase in the absorption maximum at 242 nm, indicating the modification of histidine residues. Statistical analysis of the residual enzyme activity and the extent of modification indicated modification of one essential histidine residue to be responsible for loss of the catalytic activity of EAC cell GA3PD. DEPC inactivation was protected by substrates, D-glyceraldehyde-3-phosphate and NAD, indicating the presence of essential histidine residue at the substrate-binding region of the active site. Double inhibition studies also provide evidence for the presence of histidine residue at the active site. Page(s): 7-13

Transport studies through liquid membranes of ciprofloxacin and norfloxacin

2009-03-31T16:30:46Z

Title: Transport studies through liquid membranes of ciprofloxacin and norfloxacin Authors: Nagappa, A N; Kole, P L; Pandi, P V; Patil, R T Abstract: Ciprofloxacin and norfloxacin, the widely used drugs have been shown to generate liquid membranes in series with a supporting membrane (Sartorius celluose acetate microfiltration membrane). Transport of dextrose and ions, such as NH₄⁺, Mg²⁺, Ca²⁺, K⁺ and PO₄³⁻ has been studied in the presence of liquid membranes generated by these drugs. The data obtained on the modification in the permeability of dextrose and ions in the presence of liquid membrane indicate the significance of liquid membranes in passive transport. Page(s): 48-52