NOPR Collection:

Identification of flavonoids from the aquatic pteridophyte Azolla microphylla using High Performance Thin Layer Chromatography

2016-07-21T05:40:29Z

Title: Identification of flavonoids from the aquatic pteridophyte Azolla microphylla using High Performance Thin Layer Chromatography Authors: Abraham, G.; Yadav, Ravindra K; Kaushik, G.K. Abstract: Among the several phytochemical constituents flavonoids are important as they exhibit considerable antioxidant activity and are also reported to have antimicrobial activity. Hence in this study HPTLC analysis was carried out to detect flavonoids using Chloroform-Ethyl acetate-Glacial acetic acid as mobile phase and quercetin and kaempferol as standard. The whole plants of Azolla microphylla showed at least eight different types of flavonoids with Rf values ranging between 0.06 to 0.97 and the flavonoids identified in the extracts were quercetin and kaempferol. Page(s): 1125-1128

Comparative Study on Growth, Feed Consumption and Survival of Spiral babylon Babylonia spirata Linnaeus, 1758 (Mollusca: Gastropoda) Fed with Formulated Diets

2016-07-21T05:38:24Z

Title: Comparative Study on Growth, Feed Consumption and Survival of Spiral babylon Babylonia spirata Linnaeus, 1758 (Mollusca: Gastropoda) Fed with Formulated Diets Authors: Chelladurai, G.; Mohanraj, J.; Mani, M.Sasirekha Abstract: A growth trial was conducted to determine the effects of the dietary protein level on growth, feed utilization and survival of Babylonia spirata. Triplicate groups of 50 animals (with an initial average weight of 49.24 ± 0.8 g) were stocked in 50-l tanks and fed to apparent satiation twice daily for three months with two experimental diets of 30% and 40% protein content and control diet of fresh meat clam. Growth of snails that were fed with natural food was not significantly (P<0.05) higher than those fed with the experimental diets. There was no significant difference (P>0.05) in growth and Feed Conversion Ratio between snails that were fed with experimental diets. Present study infers that a diet containing 30% and 40% of protein content may be recommended for the growth of B. spirata. Page(s): 1120-1124

Antibiogram of coral reef associated molluscs from tuticorin coast, gulf of mannar, India

2016-07-21T05:34:57Z

Title: Antibiogram of coral reef associated molluscs from tuticorin coast, gulf of mannar, India Authors: Chelladurai, G.; Mohanraj, J.; Kumar, I.Vijaya Abstract: To study the Antibiogram of crude methanolic extracts of five marine gastropods and two bivalves collected from south east coast of India (Gulf of Mannar). The disc methods (mm) and minimum inhibitory concentration (MIC) technique were used to study the antibacterial activity of these molluscs against six bacterial pathogens. The crude methanolic extracts of all the molluscs showed significantly activity against one or more bacterial tested. MIC of crude extract was found in the range of 20-40 mg / mL against pathogenic bacterial strains. Chicoreus ramosus, Murex trapa and Arca conplanta exhibited broad spectrum activity against all the bacterial tested (6-15 mm). The bivalve of Chama reflexa methanolic extract was weakly active against Escherichia coli, Salmonella typhi and S. paratyphi but demonstrated good activity against Vibrio cholera, V.parahaemolyticus and Aeromonas hydrophilla. The result of the present study coral reef associated molluscs screened exhibited bioactive potential activity against six bacterial strains. Page(s): 1112-1119

A report on thermostable alkaline protease producing bacteria from a terrestrial thermal spring

2016-07-21T05:32:54Z

Title: A report on thermostable alkaline protease producing bacteria from a terrestrial thermal spring Authors: Pathak, Anupama P.; Rathod, Mukundraj G. Abstract: Five efficient protease producing moderate thermophiles were isolated from Unkeshwar (19°85’ N and 78°25’E) thermal spring located in Nanded district of Maharashtra. The isolates were identified as Bacillus firmus, Bacillus aminivorans, Bacillus sterothermophilus, Bacillus brevis and Pseudomonas oleovorans using biochemical techniques. Being an efficient protease producer, Pseudomonas oleovorans was selected and further identified using 16s rRNA molecular analysis method as ‘Uncultured bacterium clone APP30’ and sequence was deposited in genebank nucleotide repository under accession number KC733812. The protease extracted and partially purified from APP30 showed remarkable caseinolytic activity at 55° C temperature and pH 9.0. The extracted protease also showed stability in presence of Chloroform, Ethanol, Butanol, Hexane, Toluene and Triton X-100 at 1 % (v/v) concentration and compatibility with different commercial detergents. Efficient destaining of blood stained cotton cloth piece was also recorded by partially purified protease. Enhanced proteolytic activity was observed in presence of CaCl2, MgSO4, KCl and NaCl at 1 % (w/v) concentration. Characterization of partially purified protease was carried out. Molecular weight of partially purified protease was determined as 28 kda. Page(s): 1104-1111