NOPR Collection:

Microbial Production of Astaxanthin pigment using Marine shrimp

2016-07-22T08:36:27Z

Title: Microbial Production of Astaxanthin pigment using Marine shrimp Authors: Bhavsar, S. P.; Singh, K. G.; Sharma, L. P. Abstract: In this study, shrimp waste collected from local hotels of Nashik and Trimbakeshwar, was used as a source of Astaxanthin and Lactobacillus spp. Overall, 4 isolates of Lactobacillus from various shrimp body parts viz., head, stomach, tail etc. were obtained and identified using morpho-biochemical characteristics. Astaxanthin extracted from dried shrimp waste using hexane/acetone gave 4% recovery (~0.4g/10g of shrimp) and microbial fermentation using Lactobacillus isolates showed enhanced astaxanthin production. Detection of the extracted pigment was done using thin layer chromatography (TLC). The concentrate was subjected to TLC using silica gel G250 sheet and run with solvent system of hexane:acetone (2:3). Thin layer chromatographic separation of carotenoid extract from shrimp waste produced a distinct band at Rf ~0.3 corresponding to free Astaxanthin. It was further characterized using spectrophotometry with absorption maxima at 465 nm and using High Performance Liquid Chromatography (HPLC). Preliminary study was done on microbial fermentation for astaxanthin production so as to select the efficient microbial culture and significant fermentation factors. This investigation concludes that Lactobacillus spp. are efficient candidate for astaxanthin fermentation and that soyabean extract supports maximum astaxanthin yield as indicated by the FFD . Page(s): 352-357

New distributional records of free-living marine nematodes from Indian waters II. Monhysterids

2016-07-22T08:33:55Z

Title: New distributional records of free-living marine nematodes from Indian waters II. Monhysterids Authors: Ansari, K.G.M.T.; Lyla, P.S.; Khan, S. Ajmal Abstract: Monhysterids were found to be the second most dominant order among the free-living marine nematodes which were collected from the continental shelf region of southeast coast of India during FORV “Sagar Sampada” Cruise no. 260 consisting 63 out of 192 species. Among these nine species such as Thalassomonhystera venusta, Cobbia trefusiaeformis, Theristus ensifer, T. longus, Sphaerolaimus balticus, S. gracilis, Astomonema southwardorum, Siphonolaimus cobbi and Araeolaimus elegans were found to be new distributional records for the Indian waters. Page(s): 342-351

Marine sponges as biological indicator of oligotrophic Andaman waters

2016-07-22T08:32:15Z

Title: Marine sponges as biological indicator of oligotrophic Andaman waters Authors: Kiruba-Sankar, R.; Chadha, N.K.; Dam-Roy, S.; Sawant, Paramita Banerjee; Saharan, Neelam; Krishnan, P. Abstract: Surveys on marine sponges conducted vide SCUBA at South Andaman (North Bay, Chidiyatapu, Havelock and Neil Island) at three different depth zones (5, 10 and 15m) using randomly placed quadrants(1x1m2). Marine sponge recorded in the surveys showed Carteriospongia foliascens, a phototrophic species, which mainly relies on the translocation of nutrients from their symbiotic cyanobacteria was observed in all depths and is well known as biological indicator of oligotrophic waters. Page(s): 338-341

Developmental stages observed during experimental culture of the egg bearing cyclopoid copepod Oithona similis (Claus, 1866)

2016-07-22T08:29:38Z

Title: Developmental stages observed during experimental culture of the egg bearing cyclopoid copepod Oithona similis (Claus, 1866) Authors: Jean, Jose J; Lincy, Alex; Lipton, A P; Chandran, A Abstract: Experimental culture of the marine cyclopoid copepod Oithona similis were made to assess their different developmental stages and developmental time in laboratory conditions. Cost-effective techniques for breeding, growth, development of O. similis and relevant micro algal feed production were ascertained by suitable experiments. The results revealed that the span of life cycle stages of O. similis after fertilization lasted for 23 days. The life cycle had five naupliar stages such as: N1 – 1st day; N2 – 3rd day; N3 – 5th day; N4 – 8th day and N5 – 10th day. The naupliar stage was followed by six copepodid stages (C1 – 12th day; C2 – 14th day; C3 – 16th day; C4 – 19th day; C5 – 21st day; C6 – 23rd day) and a single pre-adult stage in the laboratory conditions. Maximum survival of 30% copepodids was attained during 23 days of rearing in culture flasks of 1ℓ capacity provided with micro algal diet size <5µ. Page(s): 333-337