http://nopr.niscpr.res.in/handle/123456789/45708 2026-10-09T02:20:43Z 2026-10-09T02:20:43Z Jolly, Monica Krishnan, Ved Vinutha, T Praveen, Shelly Sachdev, Archana http://nopr.niscpr.res.in/handle/123456789/45723 2019-01-17T06:18:01Z 2018-12-01T00:00:00Z

Title: Targeted genome editing with CRISPR/Cas9: a step towards generating nutritionally rich soybean Authors: Jolly, Monica; Krishnan, Ved; Vinutha, T; Praveen, Shelly; Sachdev, Archana Abstract: Phytic acid is 75-80% of the total phosphorus in cereal and legume seeds but maximum portion of it is not accessible as the monogastric animals don't have phytase in their digestive tracts which is important for hydrolysis of the phytate molecule. This leads to eutrophication due to the influx of phosphorus from undigested animal waste which is the major source of agricultural phosphorus runoff. Also the polyanionic antinutrient, phytic acid binds to mineral cations and makes them unavailable for absorption by animals leading to mineral deficiencies. Low phytate soybean is thus desirable not only for improving human health but also for reducing the environmental phosphorus load. The implications of phytic acid on the environment, human nutrition and livestock industry have sparked numerous nutritional studies focussing on reduction of its levels. Unfortunately, the negative and altered phenotypes obtained by classical breeding mutations and reverse genetics approaches necessitate the usage of a more targeted and efficient biotechnological intervention for tissue-specific knockdown of phytic acid, obliterating the negative impacts on basal metabolic functions. The steps undertaken in the present study are for developing low-phytate soybean using the latest developed, targeted genome editing tool, CRISPR/Cas9 system for precise and efficient editing of the IPK2 gene of phytic acid biosynthesis pathway. A CRISPR/Cas9 construct for editing the IPK2 gene, involved in phytic acid biosynthesis was successfully designed and generated. Page(s): 63-68

2018-12-01T00:00:00Z Upadhyay, Ruby Pandey, Kamlesh http://nopr.niscpr.res.in/handle/123456789/45722 2019-01-17T06:20:51Z 2018-12-01T00:00:00Z

Title: Importance of membrane filtration in water purification Authors: Upadhyay, Ruby; Pandey, Kamlesh Abstract: Water is an essential component for life on earth but in recent time's water pollution is a serious environmental issue. The treatment of this water is of special concern due to the scarcity of water and the presence of recalcitrance and persistence of pollutants in water. In recent years, various methods for pollutants removal from waste water have been extensively studied. The present paper reviews the use of various types of membrane methods to remove pollutants from waste water. These membrane methods include microfiltration, ultrafiltration, nanofiltration and reverse osmosis methods. About 161 published articles are reviewed in this paper. Page(s): 69-93

2018-12-01T00:00:00Z Patel, Ambalal V. http://nopr.niscpr.res.in/handle/123456789/45721 2019-01-17T06:22:04Z 2018-12-01T00:00:00Z

Title: Functional level data acquisition requirement specification formulation for embedded systems: challenges, experiences and guidelines Authors: Patel, Ambalal V. Abstract: With the advancement in technology, many features of the large scale application systems are being embedded and miniaturized. This has led to investment of significant efforts on testing and verification of the developed embedded system. The data / signals acquired at intermediate stages of the system aids in analysis and debugging in accelerated manner. This article presents some guidelines for formulating the data acquisition / test points requirements of the core functional module (algorithms) and relevant aspects which are required to be considered during the development to cater for testing, evaluation and subsequent expansion or up-gradation of the system. The guidelines are proposed based on the experience gained over a period of time while working on safety critical embedded systems. The relevant experiences are also shared with the help of a few illustrative examples. Page(s): 94-105

2018-12-01T00:00:00Z Chalisgaonkar, Deepa Jain, Sharad K Nema, M K Mishra, P K http://nopr.niscpr.res.in/handle/123456789/45720 2019-01-17T06:23:05Z 2018-12-01T00:00:00Z

Title: Quantification of water footprint of National Capital Territory (NCT) of Delhi Authors: Chalisgaonkar, Deepa; Jain, Sharad K; Nema, M K; Mishra, P K Abstract: Over, exploitation of fresh,water caused by the increasing population and urbanization over the past few decades has resulted in water scarcity in National Capital Territory (NCT) of Delhi. Water footprint (WF) is a useful tool to better understand the linkages between humanity's activities and their growing pressure on the freshwater resources. It indicates the amount of water required to produce all the goods and services by the individual or community, or geographic area directly and indirectly (virtual water). It is measured in terms of water volumes consumed (evaporated) and/or polluted per unit of time. Many studies have been carried out pertaining to the water requirement of NCT of Delhi. The present study is focused on the assessment of the WF of NCT of Delhi. The total WF of NCT of Delhi for the year 2010 has been assessed as 15,926 MCM per annum. Out of this 6530, 780 and 865 MCM per annum has been contributed from domestic, agriculture and industrial sector, respectively and rest (7751 MCM per annum) is as virtual water import. Certain assumptions were made due to non-availability of some of the data. With the availability of more data, the assessment can be improved. Quantification of WF of NCT of Delhi indicates that domestic sector is the major water consumer. Sector wise results can facilitate authorities to develop improved management policies, action plans and strategies for better management of fresh water resources. Page(s): 106-114

2018-12-01T00:00:00Z