http://nopr.niscpr.res.in/handle/123456789/58531 2026-10-11T12:23:02Z 2026-10-11T12:23:02Z Lal, Nishita G, Anilkumar http://nopr.niscpr.res.in/handle/123456789/61882 2023-05-09T11:34:48Z 2023-05-01T00:00:00Z

Title: Isolation, sequencing and phylogenetic analysis of the two major functional domains of the ecdysteroid receptor gene (EcR) of a field crab inhabiting Indian peninsula: A first-time report Authors: Lal, Nishita; G, Anilkumar Abstract: The major functional domains (DNA binding domain, DBD and ligand-binding domain, LBD) of the ecdysteroid receptor gene (EcR), of the field crab (Parathelphusa hydrodromous) inhabiting the Indian peninsula are being elucidated in this paper. Oligo (dT) primed cDNA synthesis, PCR amplification and multiple sequence alignment (MSA) revealed that PhEcRDBD comprises 189 bp, showing 100% identity in amino acid sequence with those of all the six brachyuran crabs studied to date. PhEcRLBD (654 bp) has shown ~90 - 99% identity with Gecarcinucid/Parathelphusid field crabs; with other brachyurans and non-brachyurans, the identity declined perceptibly (~73 - 90%). Compared to PhEcRDBD, PhEcRLBD is less conserved, apparently due to its multiple functions such as homo/hetero dimerization and transcriptional activation/repression, in addition to ligand binding. MSA could locate non-synonymous substitutions, signifying differences in amino acid content existing among various taxa; this difference, however, does not seem to affect the efficacy of the ligand binding, evidenced by in silico docking studies. Interestingly, in addition to providing us with basic information on ecdysteroid receptors, the present MSA and phylogenetic analysis implicate the existence of parallelism between LBD sequence diversity and the habitat of the animal in question, in the order of – Gecarcinucid/Parathelphusid (field) crabs, non-Gecarcinucid/Parathelphusid (estuarine/marine crabs), non-brachyurans and non-crustacean arthropods, respectively. Page(s): 335-342

2023-05-01T00:00:00Z Parul Bist, Basanti Singh, Satyendra P Sharma, Barkha Jain, Udit Mishra, Raghavendra P Kumar, Ashok http://nopr.niscpr.res.in/handle/123456789/61881 2023-05-24T10:28:45Z 2023-05-01T00:00:00Z

Title: Virulence characterization and phylogenetic analysis of non-O157 vero toxin producing Escherichia coli (VTEC) isolated from cattle in India Authors: Parul; Bist, Basanti; Singh, Satyendra P; Sharma, Barkha; Jain, Udit; Mishra, Raghavendra P; Kumar, Ashok Abstract: The virulence potential and phylogenetic analysis of non-O157 Vero toxin-producing Escherichia coli (VTEC) serogroups isolates was studied. A total of 540 samples comprise of cattle faeces (378 samples), cow raw milk (108 samples) and farm soil (54 samples) were processed for the isolation of E. coli and characterized phenotypically by Vero cell cytotoxicity assay (VCA). The positive isolates subjected to serotyping and molecular characterization carried out by multiplex PCR followed by phylogenetic analysis. The sixty VCA positive isolates were serotyped into 18 different serogroups of non-O157 VTEC with prevalence of 11.11% (60/540) while none of isolates belonged to O157 serogroup. The two serogroups (O26 and O121) members of Top-6 non-O157 VTEC were also reported in serotyping. The five different gene combinations of virulent genes were revealed in 48.33% (26/60) isolates while rest were single verotoxin gene bearer in virulence profile. The dominance of vt2 gene (43.33%, 26/60) was noticed over the vt1 (35%, 21/60), hlyA (33.33%, 20/60) and eaeA (18.33%, 11/60) genes while, coexistence of verotoxin genes (vt1 and vt2) was revealed in 21.66% (13/60) isolates. Phylogenetic analysis showed the 100% identity and least divergence among the sequences of vt1 gene in comparison with other studied genes, therefore, the vt1 moiety can be used as diagnostic tool. Thus, findings provide the genetic diversity profile of non-O157 VTEC from cattle of Indian region that may be valuable in diagnostic strategies for control of these serogroups around the globe. Page(s): 343-354

2023-05-01T00:00:00Z Faria, Nicola Ghadi, Sanjeev C. http://nopr.niscpr.res.in/handle/123456789/61880 2023-05-09T11:28:59Z 2023-04-01T00:00:00Z

Title: Isolation of a thermophilic Meiothermus sp. strain JE2 and its infective phage vB_Msp_JE2 from Rajwadi hot spring, India Authors: Faria, Nicola; Ghadi, Sanjeev C. Abstract: A thermophilic, red-pigmented aerobic bacteria designated as strain JE2 was isolated from the water sample of the Rajwadi hot spring. Bacterial strain JE2 was Gram-negative, rod-shaped that grew optimally at pH 7.5 and 60°C. Molecular identification of the 16S rRNA sequence indicated that bacterial strain JE2 formed a coherent cluster with genus Meiothermus. Subsequently, a lytic bacteriophage designated as vB_Msp_JE2 was isolated from the Rajwadi hot spring that could be propagated using Meiothermus sp. strain JE2. One-step growth curve using Meiothermus sp. strain JE2 as host demonstrated a latent period of 70 min and a burst size of 20. Bacteriophage vB_Msp_JE2 was stable at pH 6.0 - 8.0. Additionally, the phage was thermostable at 60°C and was completely inactivated above 75°C. Since, most microbial studies have been reported predominantly from the Himalayan geothermal province, the present study reports the association of a unique culturable pigmented thermophilic bacterial host and bacteriophage from Rajwadi hot spring present in the West coast geothermal province, India. Page(s): 355-363

2023-04-01T00:00:00Z Kumar, Amresh Kumar, Vaibhav Maheshwari, Chirag Garg, Nitin Kumar Mawlong, Ibandalin Tyagi, Aruna http://nopr.niscpr.res.in/handle/123456789/61879 2023-05-11T09:31:39Z 2023-05-01T00:00:00Z

Title: Nuclear localization and target genes analysis of an ABA and drought-responsive transcription factor (RDA1) in rice Authors: Kumar, Amresh; Kumar, Vaibhav; Maheshwari, Chirag; Garg, Nitin Kumar; Mawlong, Ibandalin; Tyagi, Aruna Abstract: Apetala2/Ethylene Responsive Fector (AP2/ERF) family transcription factors (TFs) play an important role in controlling cellular processes and regulating important functions of plant growth and development as well as responses to environmental stimuli. AP2/ERF transcription factor responsive to drought and ABA (RDA1) from rice genotype N22 was chosen for this study. Transient expression revealed that RDA1 was localized in the nucleus. Quantitative expression studies of RDA1 under three different treatments namely drought, ABA and drought + ABA at three different stress levels showed maximum expression (3.92 fold) under drought +ABA treatment. Further, higher expression was found in N22 (drought tolerant) compared to IR 64 (susceptible) at different stress levels. Temporal expression revealed higher relative expression in leaf tissue compared to root tissue in both cultivars. RDA1 acts as a cytokinin primary response gene as per in silico characterization and many of its target genes are involved in transcriptional, post-transcriptional and translational regulation of protein biosynthesis. Relative co-expression studies for target genes revealed positive co-expression of GAMYB-binding protein, leucyl tRNA synthetase and negative co-expression of ribosomal protein L7, crooked neck (CRN) protein and XPAbinding protein with RDA1. Differential co-expression of WD40 and DUF382 domain protein was observed in root and leaf tissue with RDA1. Page(s): 364-374

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