NOPR Collection: <b>[Pages 819-945]</b><b>[Pages 819-945]</b>http://nopr.niscpr.res.in/handle/123456789/585622026-10-11T10:12:00Z2026-10-11T10:12:00ZGrowth promoting activities of antagonistic bacterial endophytes from Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg.Abraham, AmithPhilip, ShajiJoseph, JobyS, PramodJacob, Kuruvilla CSindhu, RaveendranPandey, AshokSang, Byoung-InJayachandran, Kochupurackalhttp://nopr.niscpr.res.in/handle/123456789/585742021-12-02T07:02:23Z2021-12-01T00:00:00ZTitle: Growth promoting activities of antagonistic bacterial endophytes from Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg.
Authors: Abraham, Amith; Philip, Shaji; Joseph, Joby; S, Pramod; Jacob, Kuruvilla C; Sindhu, Raveendran; Pandey, Ashok; Sang, Byoung-In; Jayachandran, Kochupurackal
Abstract: Rubber plantations are known to udergo various biotic and abiotic stresses. However, the symbiotic bacterial endophytes
that inhabit them provide protection. Here, we isolated bacterial endophytes from the rubber tree, Hevea brasiliensis (Willd.
ex A.Juss.) Müll.Arg. and studied their antagonistic activity against major pathogens such as Phytophthora meadii,
Corynespora cassiicola and Corticium salmonicolar. The antifungal metabolites such as HCN, siderophores and salicylic
acid were produced by the antagonistic endophytes under in vitro conditions. Bioassay showed the growth promotion by a
consortium of selected antagonistic endophytes in H. brasiliensis seedlings. The photosynthetic efficiency of seedlings
increased after endophyte inoculation. Endophyte-treated plants showed accumulation of starch granules in root tissues. The
selected antagonistic isolates belong to Bacillus sp. and Pseudomonas sp. The study revealed the biocontrol and growth
promoting potential of bacterial endophytes from H. brasiliensis.
Page(s): 827-8332021-12-01T00:00:00ZPromising antimicrobial protein from KlebsiellaShahadani, Ashwini A.M, RajyalakshmiHadagali, AshokaVinjamuri, Saishahttp://nopr.niscpr.res.in/handle/123456789/585732021-12-02T06:54:12Z2021-12-01T00:00:00ZTitle: Promising antimicrobial protein from Klebsiella
Authors: Shahadani, Ashwini A.; M, Rajyalakshmi; Hadagali, Ashoka; Vinjamuri, Saisha
Abstract: Increasing antibody resistance necessitates the use of new antimicrobial products. Antimicrobial peptides (AMP`s) And
Proteins are a promising alternative to antibiotics currently being used due to their high degree of specificity & wide range
of action Here we report a novel antimicrobial protein from iron reducing bacteria isolated from the coffee estate soils of
Coorg district, Karnataka, India. Iron reducing bacteria are known to be antagonistic to others due to the presence of
Pseuderophores. It makes them a good source of antimicrobial active principles. Fifteen isolates were obtained by
selectively screening for iron reduction. These isolates were tested for antimicrobial activity against Gram positive
[Staphylococcus aureus (MTCC3160), Bacillus cereus (MTCC6620)] & Gram negative [Escherichia coli (MTCC1650),
Salmonella typhi (MTCC3224) and Pseudomonas aeruginosa (MTCC2453)] bacteria. Of the 15 isolates, one isolate, CBI-8,
which showed good antimicrobial activity, was selected for further studies. The isolate was identified by MALDI-TOF &
16S rDNA sequencing as Klebsiella variicola/quasipneumonae (strain IS93) (genebank - MN814029). To induce and
augment the production of antimicrobial molecules, CBI-8 was grown in the presence of Salmonella typhi and the spent
medium was subjected to ammonium sulphate precipitation. The fractions which showed promising activity were further
subjected to preparatory HPLC. The distinct bands obtained in the SDS-PAGE corresponding to the HPLC peak that showed
antimicrobial activity from the induced sample was analyzed by Mass Spectrometry. Antimicrobial Peptide Database
(APD3) calculator, Collection of Anti-Microbial Peptides (CAMPR3) software used to predict AMPs in the proteins
identified from MS data.
Page(s): 834-8412021-12-01T00:00:00ZSynergistic antibacterial activity of mycosynthesized AgNPs with antibiotics against multidrug resistant Pseudomonas aeruginosa and investigation of protein profileDastidar, Madhumita GhoshNallal, V. Uma MaheshwariRazia, Mhttp://nopr.niscpr.res.in/handle/123456789/585722021-12-02T06:53:23Z2021-12-01T00:00:00ZTitle: Synergistic antibacterial activity of mycosynthesized AgNPs with antibiotics against multidrug resistant Pseudomonas aeruginosa and investigation of protein profile
Authors: Dastidar, Madhumita Ghosh; Nallal, V. Uma Maheshwari; Razia, M
Abstract: Silver nanoparticles (AgNPs) have been identified as promising bactericidal agents especially towards multidrug
resistant bacteria (MDR). Fungi are excellent biogenic sources for synthesizing AgNPs with antibacterial activity. In the
present study, AgNPs were synthesized using Aspergillus niger extract and characterized. Individual and synergistic
antibacterial activities of AgNPs were tested against isolated hospital strain of MDR Pseudomonas aeruginosa by disc
diffusion assay. SDS-PAGE analysis was performed to analyse the protein expression before and after treatment with
AgNPs. Results revealed the successful synthesis of AgNPs using A. nigerby 24 hrs and the average particle size was
measured as 18.72±0.2 nm. AgNPs were predominantly spherical and monodispersed. 18±0.05 mm zone of inhibition was
observed for P. aeruginosa when treated with AgNPs alone whereas synergistic treatment of synthesized AgNPs along with
Tetracycline showed impressive antibacterial activity (27.32±2 mm). Protein expression of P. aeruginosa varied before and
after treatment which was evident from the results. This indicated the effective breakdown of cell wall and cellular proteins
on the synergistic treatment of AgNPs and antibiotics. Thus the study demonstrates a feasible and eco-friendly approach to
synthesize AgNPs with enhanced antibacterial activity against MDR bacterial strains.
Page(s): 842-8482021-12-01T00:00:00ZBioactive molecules and the antidiabetic efficacy of Memecylon randerianum — an ethnomedicinal plant from the Western GhatsLakshmi, MVSwapna, TShttp://nopr.niscpr.res.in/handle/123456789/585712021-12-02T06:41:11Z2021-12-01T00:00:00ZTitle: Bioactive molecules and the antidiabetic efficacy of Memecylon randerianum — an ethnomedicinal plant from the Western Ghats
Authors: Lakshmi, MV; Swapna, TS
Abstract: Diabetes is one of the most chronic diseases and a leading cause of death even though it is preventable. There are
multiple therapeutic strategies like daily intake of drugs, insulin treatment and islet transplantation to manage severe diabetic
complications. But the plant based therapy with regular exercise can improve diabetic control with reduced medication. The
Malabar Memecylon, Memecylon randerianum S.M. Almeida & M.R. Almeida, is a medicinal shrub widely distributed in
the Western Ghats of Kerala. Leaves are the most utilized plant part in traditional medicine. In vitro antidiabetic activity and
HR-LCMS-QTOF analysis of the leaves, along with the screening of phytochemicals, has been executed in the present
investigation. The methanolic extract showed the highest presence of phytochemicals such as flavonoids, tannins, saponins
etc. The quantitative screening revealed that saponins (211.16±1.9 mg/g) and flavonoids (209.74±2.12 mg/g) were highly
concentrated, and the leaf extract showed significant dose-dependent inhibition on α-amylase and α- glucosidase. The
glucose uptake in L6 cell lines was found to be increased by 25% compared to untreated cells (control) at 100 μg/mL sample
concentration. HR-LCMS-QTOF analysis detected nine major compounds and showed that leaves of the plant could be used
as an effective therapeutic agent against the most common type 2 diabetes.
Page(s): 849-8572021-12-01T00:00:00Z