<b>[Pages 641-738]</b> http://nopr.niscpr.res.in/handle/123456789/60437 2026-10-10T16:56:52Z 2026-10-10T16:56:52Z Madhavan, Aravind KB, Arun Sindhu, Raveendran Binod, Parameswaran Awasthi, Mukesh Kumar Pandey, Ashok http://nopr.niscpr.res.in/handle/123456789/60448 2022-09-01T06:42:00Z 2022-09-01T00:00:00Z

Title: Aspergillus spp., a versatile cell factory for enzymes and metabolites: Interventions through genome editing Authors: Madhavan, Aravind; KB, Arun; Sindhu, Raveendran; Binod, Parameswaran; Awasthi, Mukesh Kumar; Pandey, Ashok Abstract: Aspergillus sp. is widely distributed in nature and plays significant roles in the degradation of lignocellulose biomass and extensively used in bioprocess and fermentation technology and many species are also a generally regarded safe. Many of the Aspergillus species are established cell factories due to their inherent capacity in secreting large number of hydrolytic enzymes. With the advent of next generation genomic technologies and metabolic engineering technologies, the production potential of Aspergillus cell factory has improved over the years. Various genome editing tools has been developed for Aspergillus like engineered nucleases, zinc finger nucleases, TALEN and CRISPR-Cas9 system. Currently, the CRISPR/Cas9-based technique is extensively used to enhance the effectiveness of gene manipulation in model system Aspergillus nidulans and other strains like Aspergillus oryzae, Aspergillus niger and Aspergillus fumigatus. This review describes the recent developments of genome editing technologies in Aspergillus the synthesis of heterologous proteins and secondary metabolites in the Aspergillus species. Page(s): 647-658

2022-09-01T00:00:00Z Srivastava, Rakesh Bajpai, Rajesh Khan, Zeba Singh, Surendra Pratap Mehrotra, Rajesh Dubey, Neeraj Kumar http://nopr.niscpr.res.in/handle/123456789/60447 2022-09-07T07:05:47Z 2022-09-01T00:00:00Z

Title: Insight into strigolactone hormone functions in plant parasitic weeds: a regulatory perspective Authors: Srivastava, Rakesh; Bajpai, Rajesh; Khan, Zeba; Singh, Surendra Pratap; Mehrotra, Rajesh; Dubey, Neeraj Kumar Abstract: The strigolactones (SLs) are plants hormones that have multiple functions in architecture and development. The roles of SLs in shoot branching and stem secondary growth of autotrophic plants are established. SL is also involved in the interaction between root parasitic plants and their host plants. SLs are exudates by the root of the host plant in search of a fungal partner for symbiotic association, while parasitic plants utilize this facility to detect the host root. The first formed tubercle of Philapanhche, whose germinations are driven by host-derived SLs, exudates parasitic derived SLs (PSLs) and could encourages germination of the adjacent parasitic seeds, resulting in parasite cluster formation. The existence of aboveground spikes in clusters suggests an intriguing approach for increasing parasite population by amplifying PSLs, which result in massive parasitic seed germination. PSLs probably have a role in the increased branching of Broomrapes opposing the host plant, resulting in the parasites' clustered appearance aboveground. This review highlights the distinct roles of SLs and PSLs, and their potential role in host-parasitic interaction. Page(s): 659-666

2022-09-01T00:00:00Z Sharma, Meenu Pandita, Monika Shoket, Heena Bairwa, Narendra K http://nopr.niscpr.res.in/handle/123456789/60446 2022-09-01T06:34:21Z 2022-09-01T00:00:00Z

Title: F-box motif encoding genes as targets for the development of stress-tolerant traits in Saccharomyces cerevisiae Authors: Sharma, Meenu; Pandita, Monika; Shoket, Heena; Bairwa, Narendra K Abstract: Stress tolerance is a useful trait actively sought by the bioprocess industry for biofactories dealing with bioconversion of varied raw materials or carbon sources. Fungal or yeast species are useful in bioconversion and are sustainable bioresources for biochemicals and biofuel production. Genetic manipulation strategies are in practice to enhance the tolerance against stress agents for the improved bioconversion process. In this review, we highlight the importance of the F-box motif encoding genes and their interactions in imparting the stress tolerance phenotype to the yeast species. The F-box motif proteins constitute a part of the SCF-E3 ligase complex and are involved in the recruitment, and ubiquitination, followed by degradation of the substrate proteins by the 26S proteasome. It highlights the current scenario on the F-box motif encoding genes and their interaction partners as targets for the stress tolerance phenotype in the yeast and plant species and their utility in the bioconversion processes. Page(s): 667-671

2022-09-01T00:00:00Z Vishvakarma, Reena Vimal, Archana Mishra, Abha Sharma, Poonam Gaur, Vivek Kumar http://nopr.niscpr.res.in/handle/123456789/60445 2022-09-01T06:30:44Z 2022-09-01T00:00:00Z

Title: Trametes versicolor (L.) Lloyd as a source of thermostable serine protease: production and characterization Authors: Vishvakarma, Reena; Vimal, Archana; Mishra, Abha; Sharma, Poonam; Gaur, Vivek Kumar Abstract: Proteases are ubiquitously present and are among the largest groups of commercially important enzymes. Here, we investigated a wood-rot basidiomycete Trametes versicolor (L.) Lloyd [Syn. Coriolus versicolor (L.) Quél.; Polyporus versicolor (L.) Fr.] as a source of the enzyme serine protease, its production, and optimized to obtain a higher yield of the enzyme.. The significant variables with optimized values for maximum production of the enzyme were temperature (30C), incubation time (120 h) and wheat bran (10 g). The yield increased by 30.76% by statistically optimizing the media. The optimized temperature and pH for the maximum protease activity was 50C and pH 7.0, respectively. The enzyme was purified through ion exchange (using DEAE cellulose 52 resin) and gel filtration chromatography (using Superdex 200 column). The purified enzyme had a retention time of 7 min in RP-HPLC. The enzyme was stable at a broad range of temperature (30-60C) and pH (5.0-8.0) with a half-life of 58.72 min, Vmax of 37.17 μM min/mL and Km of 0.657 mg/mL. Its activity was enhanced by Na+, Ca2+, Mg2+ ions and SDS surfactant. These properties make this enzyme a valuable candidate for industrial applications. Page(s): 672-680

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