http://nopr.niscpr.res.in/handle/123456789/62416 2026-10-11T15:37:18Z 2026-10-11T15:37:18Z Bhange, Khushboo Kumar, Ravi Ranjan Singh, Prabhakar Chaturvedi, Venkatesh http://nopr.niscpr.res.in/handle/123456789/62422 2023-08-14T09:44:15Z 2023-08-01T00:00:00Z

Title: Feather Protein Hydrolysate mediated synthesis of silver nanoparticles and assessment of anti-microbial potential of silver nanoparticle-treated silk cloth Authors: Bhange, Khushboo; Kumar, Ravi Ranjan; Singh, Prabhakar; Chaturvedi, Venkatesh Abstract: In this study, we have performed green synthesis of silver nanoparticles using Feather Protein Hydrolysate (FPH) formed by degradation of chicken feathers by Stenotrophomonas maltophilia KB13. The synthesized nanoparticles were characterized by techniques such as, UV-Vis Spectroscopy, Fourier Transformed Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy coupled with Energy Dispersive Spectroscopy (SEM- EDS) respectively. TEM and SEM-EDS confirmed the formation of silver nanoparticles. The size range was found to be 20-40 nm. FTIR analysis suggested the role of amino acids and proteins present in FPH during the synthesis process. XRD analysis of silver nanoparticles confirmed the formation metallic silver nanoparticles with the average size of 29.957 nm. The antimicrobial activity of nanoparticles was also studied on clinically relevant pathogenic bacteria. These nanoparticles exhibited excellent anti-microbial properties. The antimicrobial property of silver nanoparticles was employed for the rescue of silk cloth from microbial deterioration. Page(s): 581-588

2023-08-01T00:00:00Z Chaudhary, Shikha Yadav, Ravi P Kumar, Shailendra Yadav, Subhash C http://nopr.niscpr.res.in/handle/123456789/62421 2023-08-14T09:41:13Z 2023-08-01T00:00:00Z

Title: Aplastic anemia patients with severe COVID-19 showed abnormal ultrastructure features on type II pneumocytes of lungs Authors: Chaudhary, Shikha; Yadav, Ravi P; Kumar, Shailendra; Yadav, Subhash C Abstract: Type-II-pneumocytes from the bronchoalveolar fluids of aplastic anemia (AA) patients with COVID-19-induced acute respiratory distress syndrome (ARDS) were imaged to assess the ultrastructural alteration, especially in the number and size of the lamellar bodies. The mild (non-ARDS), and severe ARDS without comorbidities were used as reference for comparative ultrastructural analysis. The electron microscopic imaging of mild non-ARDS patients showed normal ultrastructure with surfactant-containing lamellar bodies of size range 0.5-1.00 μM with an area ranging from 0.3-0.8 μM2. The ARDS patients with diabetes and without any comorbidities showed larger lamellar bodies of the size range 0.8-1.4 μM with an area range from 0.5-1.50 μM2. However, AA patients revealed normal and giant lamellar bodies. These giant lamellar bodies showed a size range of 2.0-4.0 μM with an area ranging from 3.0- 5.0 μM2 with a lesser number/cell. This study reports the abnormal ultrastructural alteration of type II pneumocytes in AA patients with COVID-19. Page(s): 589-595

2023-08-01T00:00:00Z Sarwar, Saba Quadri, Javed Ahsan Shariff, Ahmadulla http://nopr.niscpr.res.in/handle/123456789/62420 2023-08-14T09:37:21Z 2023-08-01T00:00:00Z

Title: Urinary exosome isolation in fluorosis patients with early symptoms of nephrotoxicity Authors: Sarwar, Saba; Quadri, Javed Ahsan; Shariff, Ahmadulla Abstract: Polyureic nephropathy, a kidney disease characterized by excess thirst & frequent urination, are established manifestations of non-skeletal fluorosis. This study aimed to isolate the urinary exosomes from fluorosis patients with early renal impairment. Fluorosis patients having urinary fluoride above 1ppm were recruited as test group (Group-1). The recruited fluorosis patients were sub-divided in two groups i.e., fluorosis patients with normal renal functions (G-1A) and fluorosis paints with renal impairments (G-1B). Healthy individuals having urinary fluoride up to 1ppm were included as control (Group-2). Urinary Exosomes were isolated and correlated with fluoride toxicity associated nephrotoxicity. A significantly (P = 0.002) higher urinary fluoride (G-1A: 2.62 ± 0.94 ppm and G-1B: 3.87±1.6 ppm) in fluorosis patients were observed compared to the normal control (G-2: 0.40±0.19 ppm), (P = 0.004).The serum creatinine levels was significantly increased in G-1B (3.33±0.76 mg/dL) compared to G-1A (1.11±0.89 mg/dL), (P = 0.003) and G-2, (0.64±0.17), (P = 0.005). A significant (P = 0.001) increase in BUN were observed in G-1B, compared to G-1A and control. The urinary protein was also significantly higher in G-1A (P = 0.005) and G-1B (0.001) compared to G-2. The number of exosomes per/ml of urine in G1-B was significantly higher (P = 0.05) than the G-1A and G-2. The number of urinary exosomes in G-1A and G-1B were also significantly higher than G-2 (P = 0.05). Chronic fluoride toxicity may induce renal impairments and increased urinary excretion of exosomes. Page(s): 596-600

2023-08-01T00:00:00Z Roy, Soumen Roy, Urmila Giri Ghorai, Narayan Saha, Samir Kumar Singh, Prabhakar Mukhopadhyay, Amit Tripathy, Basudev http://nopr.niscpr.res.in/handle/123456789/62419 2023-08-14T09:47:08Z 2023-08-01T00:00:00Z

Title: Ultrastructural variations of the sagittae in two Anabantiformes fishes: Anabas testudineus (Bloch, 1792) and Trichogaster fasciata (Bloch & Schneider, 1801) Authors: Roy, Soumen; Roy, Urmila Giri; Ghorai, Narayan; Saha, Samir Kumar; Singh, Prabhakar; Mukhopadhyay, Amit; Tripathy, Basudev Abstract: The sagitta otolith is well-decorated with various species-specific peculiarities, used to support the relatedness in taxonomy and other aspects among fishes. Present study deals with the morphostructural variations of the sagitta in the Anabas testudineus and Trichogaster fasciata by light and scanning electron microscopy. As these two fishes are belonging to order Anabantiformes but their sagitta morphologies are much more varies. The sagitta of the A. testudineus is oval with complete dorsal and ventral marginal boundary, while the dorsal boundary is incomplete in the T. fasciata. The sulcus of the A. testudineus is ostio-pseudocaudal with spoon-headed ostium, whereas the sulcus in T. fasciata is ostio-caudal with a simply tubular ostium. The ostium and cauda are demarcated with a prominent constriction in the A. testudineus, while this demarcation is maintained by a virgule-like development in the T.fasciata. Presence of the orthorostrum patch, and hexangular stony pillars along the marginal sculpture in the T. fasciata is a species-specific peculiarity. A phylogenetic tree is made based on the sagitta features between these two fish species and five other Anabantiformes fishes and stated that the A. testudineus is characteristically more related to Channa sp than that of the T. fasciata. Page(s): 601-610

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