http://nopr.niscpr.res.in/handle/123456789/65187 2026-10-09T21:55:22Z 2026-10-09T21:55:22Z Nadendla, Rama Rao Chandu, U Mohan Rao, KRS Sambasiva http://nopr.niscpr.res.in/handle/123456789/65200 2025-01-07T06:08:54Z 2025-01-01T00:00:00Z

Title: Biohybrid molecules: Integrating natural and synthetic components for advanced biochemical applications Authors: Nadendla, Rama Rao; Chandu, U Mohan; Rao, KRS Sambasiva Abstract: Biohybrid molecules, a fusion of natural and synthetic components, represent a significant leap in biochemical technology. These innovative molecules harness the unique properties of both their natural and synthetic constituents, enabling new possibilities in advanced biochemical applications. The integration of biological and artificial elements offers enhanced functionality, stability, and versatility, paving the way for groundbreaking advancements in areas such as drug delivery, diagnostics, and bioengineering. This approach not only bridges the gap between biology and chemistry but also opens up new frontiers in the development of smart, responsive systems tailored for specific biomedical purposes. This could revolutionize regenerative medicine, offering new hope for patients in need of transplants or tissue repairs. The development of biohybrid molecules is still in its early stages, but the promise they hold is immense. As technology advances and our understanding of molecular interactions deepens, the potential for biohybrids to transform healthcare, environmental science, and even industrial processes becomes increasingly tangible. There is significant potential for future research to unlock these possibilities, where the boundaries between natural and synthetic components are seamlessly integratedfor the betterment of society. Page(s): 107-116

2025-01-01T00:00:00Z Kashid, Snehal Shinde, Prashali Pawar, Reshma Mishra, Ashish http://nopr.niscpr.res.in/handle/123456789/65199 2025-01-07T06:01:40Z 2025-01-01T00:00:00Z

Title: In silico molecular docking and virtual screening of natural compounds against Tubulin-7-Aminonoscapine complex (6Y6D) for estimation of anticancer potential Authors: Kashid, Snehal; Shinde, Prashali; Pawar, Reshma; Mishra, Ashish Abstract: This comprehensive study integrated in silico molecular docking and pharmacokinetic profiling to elucidate the interactions and properties of eight bioactive compounds (nimbin, azadirachtin, betasitosterol, nimbidinin, chavicol, chavibetol, curcumin, and zingiberene) from natural sources with protein 6Y6D. SwissADME and SwissTargetPrediction are used for pharmacokinetic screening, whereas, autodock vina is used for molecular docking and virtual screening. Protox3 software was used to establish each phytoconstituent's specific toxicity profile. According to the results, nimbidinin and nimbin exhibit extraordinary binding affinity without toxicity and have the potential to be further screened as lead compounds. Azadirachtin, on the other hand, exhibits the highest binding affinity with a moderate toxicity profile. Furthermore, zingiberene and chavicol exhibit low binding affinities. A detailed investigation reveals the binding modes and molecular interactions with different amino acids. Our findings may facilitate the development of novel drugs and provide a foundation for further experimental validation. Page(s): 117-130

2025-01-01T00:00:00Z Udagikar, Manasi P S, Ladke Vaibhav Changade, Jayshree V http://nopr.niscpr.res.in/handle/123456789/65198 2025-01-07T05:54:01Z 2025-01-01T00:00:00Z

Title: Evaluation of osteogenic activity of Plaksha (Ficus lacor Buch. Ham.) stem bark on human dental pulp derived mesenchymal stem cells Authors: Udagikar, Manasi P; S, Ladke Vaibhav; Changade, Jayshree V Abstract: The adverse effects and lower efficacy of synthetic drugs have increased scientists' interest in traditional medicine for managing chronic diseases like osteoporosis and osteoarthritis, which raise the risk of bone fractures. Herbal medications are increasingly used for joint disorders due to their potential benefits in reducing pain, inflammation, and stiffness, as well as their safety when used correctly. Plaksha (Ficus lacor Buch. Ham) possess a great potential in treating bone disorders. The purpose of the study is to determine the osteogenic effect of stem bark extract of Plaksha on mesenchymal stem cells. Aqueous extract of Plaksha obtained via Soxhlet Apparatus. Mesenchymal stem cells were obtained from human dental pulp; Characterized using Cell Surface Antigen Expression Analysis via Fluorescence- Activated Cell Sorting (FACS). Cytotoxicity assessed with MTT. Osteogenic potential evaluated through trilineage differentiation and gene expression analysis (RT-PCR) for Alkaline Phosphatase (ALP) and Osteonectin (OSN). Viability assay revealed that the 10 μg/mL concentration helps for trilineage differentiation of cells. Upregulation of ALP and OSN genes via RT-PCR in treated group showed the osteogenic differentiation of cells. This demonstrates the aq. Extract of FL promotes the osteogenic differentiation of MSCs and facilitate the osteogenesis. Hence the drug can be used in various bone disorders. Page(s): 131-137

2025-01-01T00:00:00Z Ahmad, Zaved Jain, Subodh Kumar Mishra, Siddhartha Kumar http://nopr.niscpr.res.in/handle/123456789/65197 2025-01-07T05:52:01Z 2025-01-01T00:00:00Z

Title: Beta-caryophyllene ameliorates environmental toxicants-induced experimental nephrotoxicity through regulation of redox system and inflammation Authors: Ahmad, Zaved; Jain, Subodh Kumar; Mishra, Siddhartha Kumar Abstract: Kidneys play important roles in the detoxification and excretion of toxic metabolites making them vulnerable to xenobiotic toxicity. We investigated the possible nephrotoxicity induced in mice by environmental toxicants diethyl nitrosamine (DEN) and carbon tetrachloride (CCl4) and their mitigation by supplementation of natural bicyclic sesquiterpene β-caryophyllene (BCP). DEN (1 mg/kg) and CCl4 (0.2 mL/kg) were given to mice and then the treatment group received BCP (30 mg/kg). After 18 weeks, DEN/CCl4-induced mice showed renal injury evidenced by significant increase in circulating kidney function markers as well as histopathological alterations. Concurrent supplementation of BCP significantly ameliorated kidney function markers and prevented renal tissue damage. BCP treatment suppressed lipid peroxidation and nitric oxide levels and enhanced the antioxidant defence mechanism in the kidneys. BCP successfully amended renal histopathological changes and improved kidney serum markers and electrolytes associated with kidney function in association with alleviation of the deteriorated renal oxidative state and antioxidant defence system. BCP modulated the expression of key inflammation markers (inducible nitric oxide synthase and cyclooxygenase-2) and apoptotic markers [caspase-3 and poly (ADP-ribose) polymerase] in the DEN/CCl4-exposed kidneys. The study concludes that exposure to environmental toxicants DEN/CCl4 caused extensive nephrotoxicity that was notably mitigated by BCP suggesting its effectiveness as an alternative therapy in renal dysfunctions. Page(s): 138-146

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