NOPR Collection:

Relationship of fluorescence and thermal emission from isolated thylakoids under light stress conditions

2004-06-01T00:00:00Z

Title: Relationship of fluorescence and thermal emission from isolated thylakoids under light stress conditions Authors: Markovic, Dejan Abstract: Simultaneous measurements of fluorescence and thermal emission have been made by a combined fluorescence and photoacoustic techniques on isolated thylakoids pretreated by a prolonged illumination of saturating light. The traces of the signals are used to calculate four characteristic parameters, energy storage, half-saturation intensity, number of photons to close reaction center, and a constant for quasi-equlibria between (re)oxidized and reduced quinone acceptors. These parameters are used to study the response of photosynthetic apparatus functioning under photoinhibition stress. The defense mechanism seems to possess an efficient cooperativity of reaction centers under stress conditions. Page(s): 604-610

Comparative effectiveness of CaNa₃DTPA and tiron along with α-tocopherol against beryllium-induced biochemical alterations in rats

2004-06-01T00:00:00Z

Title: Comparative effectiveness of CaNa₃DTPA and tiron along with α-tocopherol against beryllium-induced biochemical alterations in rats Authors: Mathur, R; Nirala, Satendra Kumar; Mathur, Asha Abstract: The therapeutic efficacy of chelating agents CaNa₃ DTPA (calcium trisodium diethylene triamine penta acetic acid) and Tiron (sodium-4,5-dihydroxy-1,3-benzene disulphonate) with and without antioxidant, α-Tocopherol was evaluated in the treatment of beryllium- induced toxicity in female albino rats. The animals were exposed to beryllium (as beryllium nitrate) at a dose of 1mg/kg (ip) once a day for 28 consecutive days followed by chelation therapy by CaNa₃ DTPA (0.1 mM/kg, ip) and Tiron (471 mg/kg, ip) with and without α-Tocopherol (25 mg/kg, orally) for 5 consecutive days after toxicant administration. Tissue biochemistry revealed severe alterations in liver and kidney. A significant fall in total protein and glycogen contents, alkaline phosphatase, adenosine tri -phosphatase and succinic dehydrogenase level was noticed. On the contrary, an elevation in acid phosphatase was recorded. The significant rise in hepatic lipid peroxidation and decreased level of hepatic reduced glutathione showed toxicity due to beryllium. CaNa₃DTPA with α-Tocopherol showed moderate therapeutic efficacy while Tiron in combination with α-Tocopherol exerted statistically more beneficial effects to reverse biochemical alterations in different variables altered due to beryllium intoxication. Page(s): 570-574

Modulatory effect of diclofenac on antispasmodic effect of pitofenone in cholinergic spasm

2004-06-01T00:00:00Z

Title: Modulatory effect of diclofenac on antispasmodic effect of pitofenone in cholinergic spasm Authors: Kulkarni, Shrinivas K; Patil, Chandrashekhar S; Jain, Naveen K; Singh, Amarjit Abstract: Biliary, ureteric and intestinal colic are extremely common clinical conditions associated with smooth muscle spasm. In the present study, antispasmodic activity was carried out against acetylcholine (10-640 ng/ml)-induced contractions on guinea pig ileum. Acetylcholine (10-640 ng/ml) induced concentration-dependent contraction of smooth muscle. Diclofenac, in varying concentration (9.4 x 10^-5 mol/l and 14.1 x 10^-5 mol/l) shifted the concentration response curve of acetylcholine to the right without sppressing the maximal response. However, in higher concentration diclofenac (18.9 x 10^-5 mol/l) blocked the response in an unsurmountable fashion. Further, analgin (11.09 x 10^-5 , 16.63 x 10^-5 and 22. 18 x 10^-5 mol/l) in equimolar concentrations did not alter the concentration response curve of acetylcholine, but in higher concentration analgin (44.36 x10^-5 mol/l) also blocked the response in an unsurmountable fashion. Pitofenone (2 .5 x 10^-6mol/l) also, shifted the concentration response curve of acetylcholine to right in a parallel fashion with no change in maximal response. The present study confirms the potent antispasmodic activity of diclofenac-pitofenone combination in comparison to analgin –pitofcnone in molar equivalcnt concentration (in comparison to diclofenac) against acetylcholine-induced contractions of guinea pig ileum. Page(s): 567-569

Antioxidant effect of curcumin in selenium induced cataract of Wistar rats

2004-06-01T00:00:00Z

Title: Antioxidant effect of curcumin in selenium induced cataract of Wistar rats Authors: Padmaja, S; Raju, T N Abstract: Wistar rat pups treated with curcumin, a natural constituent of Curcuma longa before being administered with selenium showed no opacities in the lens. The lipid peroxidation, xanthine oxidase enzyme levels in the lenses of curcumin and selenium co-treated animals were significantly less when compared to selenium co-treated animals. The superoxidase di smutase and catalase enzyme activities of curcumin and selenium co-treated animal lenses showed an enhancement. Curcumin co-treatment seems to prevent oxidative damage and found to delay the development of cataract. Page(s): 601-603

NOPR Collection:

Relationship of fluorescence and thermal emission from isolated thylakoids under light stress conditions

Comparative effectiveness of CaNa3DTPA and tiron along with α-tocopherol against beryllium-induced biochemical alterations in rats

Modulatory effect of diclofenac on antispasmodic effect of pitofenone in cholinergic spasm

Antioxidant effect of curcumin in selenium induced cataract of Wistar rats

Comparative effectiveness of CaNa₃DTPA and tiron along with α-tocopherol against beryllium-induced biochemical alterations in rats