http://nopr.niscpr.res.in/handle/123456789/16247 2026-10-12T13:19:31Z http://nopr.niscpr.res.in/handle/123456789/23311 Title: Effectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production Authors: Saha-Roy, Santa; Shende, Niraj; Kumar, Satish; Harinath, B C Abstract: Monospecific antibodies have been successfully utilized in antigen detection, which is better indicator of active infection. <i>Mycobacterium tuberculosis </i>excretory secretory (<i>M tb </i>ES) antigens such as ES 31 , ES 41 and ES 43 (31 kDa, 41 kDa and 43 kDa protein, respectively) have been shown to be present in <i>Mycobacterium tuberculosis </i>H₃₇Ra culture filtrate and are of diagnostic interest. To study the immunogenic potential of crude versus purified antigen, goat was immunized with <i>M tb </i>detergent soluble sonicate (DSS) antigen as well as purified antigen fraction (ESAS 7) containing ES 31 antigen. Both anti-DSS IgG antibody and anti ESAS 7 IgG antibody were found to be reactive with ES 31 antigen upto 1 ng concentration of antibody by ELISA. Crude DSS antigen was found to be quite effective in producing high titre antibodies and showed further high reactivity with other ES antigens (ES 41 and ES 43) of diagnostic interest. Page(s): 1196-1198 2005-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/23309 Title: NaCl induced changes in photosystem stoichiometry and photosynthetic activity of the cyanobacterium <i>Microcystis aeruginosa </i>Kütz. Authors: Adhikary, S P Abstract: Exposure to 0.4 <i>M </i>NaCl resulted in higher PS I/PS II stoichiometry and increase in the rate of photosynthesis in planktonic cyanobacterium <i>M. aeruginosa. </i>Altered ratios of PS I/PS II as well as photosynthesis and respiration were stabilized within 72 hr of exposure to salt, leading to adaptation of the organism to the changed conditions. Page(s): 1192-1195 2005-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/23308 Title: Mode of utilization of amino acids as growth substrates by <i>Azospirillum brasilense</i> Authors: Bhattacharya, Pradip Abstract: The study was undertaken to analyze the rate of uptake and utilization of various amino acids by <i>Azospirillum brasilense </i>Sp81 (RG) in a basal mineral salts solution under non-nitrogen fixing condition. These amino acids including<i> </i>other nitrogenous compounds were tested for both N- and C-sources. The kinetic constants (K_m and V_max) of uptake of some<i> </i>amino acids (e.g. lysine, arginine, proline, glutamine and glutamic acid) were exploited using a Hanes-Woolf plot, and<i> </i>discussed in the context of nitrogen starvation or both carbon and nitrogen starvation. To summarize all the kinetic data for<i> </i>these amino acids strongly suggested that the mode of these amino acids utilization in this bacterium followed the same<i> </i>general pattern, although the quantitative differences were there. A single amino acid was able to satisfy the nitrogen needs<i> </i>of this bacterium in basal mineral salts solution, and this possibility could be considered for the cost-effective growth<i> </i>medium for this bacterium in the biotechnological industry. Page(s): 1182-1191 2005-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/23307 Title: Modulatory influence of juvenile hormone analogue (JHa) and 20-hydroxy-ecdysone on lipophorin synthesis in red cotton bug, <i>Dysdercus cingulatus </i>Fabr. Authors: Mohan, K G; Muraleedharan, D Abstract: Topical supply of methoprene, a juvenile hormone analogue (JHa) caused notable morphological disturbance in insects. Topical supply of methoprene to newly emerged adult female <i>D. cingulatus </i>caused notable disturbance and induced a dramatic reduction in the total haemolymph protein pattern and lipophorin production in tissues like fat body, ovary and haemolymph. Total protein concentration in haemolymph also showed significant reduction in 1 day old insects but increased slightly as age advanced. Application of 20 -hydroxyecdysone (20-HE) to 2-day-old adult female stimulated protein synthesis intensively. Lipophorin levels in fat body and ovary also simultaneously increased. Densitometric analysis revealed that methoprene inhibits while 20-HE stimulates Jipophorin production in <i>D. cingulatus.</i> Page(s): 1176-1181 2005-12-01T00:00:00Z