http://nopr.niscpr.res.in/handle/123456789/23019 2026-10-09T03:22:45Z http://nopr.niscpr.res.in/handle/123456789/23051 Title: Isolation of poly (A⁺)<i> </i>mRNA for downstream reactions from some medicinal and aromatic plants^† Authors: Shukla, Ashutosh K; Shasany, Ajit K; Khanuja, Suman P S Abstract: In the present protocol for extraction of RNA, hexadecyltrimethylammoniumbromide (CTAB) and insoluble polyvinylpyrrolidone were used followed by LiCl precipitation, CsCl ultracentrifugation and finally poly (A⁺)<i> </i>mRNA was isolated with the help of oligo(dT)-cellulose columns. The isolated poly (A⁺)<i> </i>mRNA was found to be suitable for cDNA-AFLP and suppression subtractive hybridization applications. It is a modified and consolidated protocol based on previously described methods for isolated steps and works better for medicinal and aromatic plants. High yield of poly (A⁺)<i> </i>mRNA coupled with its amenability for downstream reactions like RT-PCR. northern blotting and cDNA synthesis for library construction is a key feature of the present protocol. Page(s): 197-201 2005-02-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/23048 Title: Early and transient induction of nitric oxide (NO) in infectious bursal disease virus infection is T-cell dependent: A study in cyclosporin-A treated chicken-model Authors: Poonia, Bhawna; Charan, Shiv Abstract: The level of nitric oxide (NO) in the supernatants of mitogen (PHA) stimulated lymphocyte cultures from infectious bursal disease (IBD) virus infected T-cell suppressed and immune competent chickens was monitored. The immune competent chickens when infected with IBD virus showed 4-6 folds increased levels of NO as compared to uninfected chickens. The levels of NO in T-cell suppressed chickens were comparable to uninfected control chickens, in spite of markedly increased hemorrhage suggesting that the muscular hemorrhage observed in IBD in not solely and directly related with NO production. The immune suppressed chickens that did not induce NO production after IBD virus infection showed more severe lesions and supported enhanced virus replication. Taken together it may be suggested that NO production after IBD virus infection, may exert antiviral effect since the immune-suppressed chickens that failed to induce NO showed more severe disease and higher magnitude of virus replication, but does not seem to correlate with the hemorrhagic lesions which in fact may be as a result of the net outcome of various host-factors and the determinants responsible for virus virulence and virus clearance. Page(s): 192-196 2005-02-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/23045 Title: Effect of alliums on total lipid in plasma and tissues of Japanese quails (<i>Coturnix coturnix japonica</i>)<i> </i>with respect to age and sex Authors: Kumar, V Girish; Surendranathan, K P; Devi, D R Gayathri; Belwadi, M R Sandhya Abstract: Dietary onion and garlic caused an increase in the level of liver and plasma total lipids in <i>Coturnix oturnix japonica. </i>This<i> </i>increase could be due to the effect of an increased feed intake, bile production, digestion and absorption that<i> </i>in turn caused an increased utilisation of dietary fat, increased transfer of dietary lipids to the liver and/or due to increased<i> </i>lipogenesis as such. However, there was no increase in the muscle lipid content. This effect in the muscle could be due to<i> </i>inhibitory effect of onion or garlic on lipoprotein lipase activity. Further, the changes in the tissue total lipid level in the<i> </i>control group due to change in age and sex were also observed. Page(s): 187-191 2005-02-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/23043 Title: Antiulcerogenic effect of <i>Justicia prostrata </i>Gamble Authors: Sanmugapriya, E; Shanmugasundaram, P; Venkataraman, S Abstract: Antiulcerogenic effect of the alcoholic (ALJP) and aqueous (AQJP) extracts of the whole plant of <i>Justicia prostrata </i>was studied in aspirin + pylorus ligated rat models and analysed for gastric volume, ulcer index, free and total acidity. Biochemical parameters like total proteins, total hexoses, hexosamine, fucose and sialic acid were also estimated. Both extracts (ALJP and AQJP) significantly reduced both the gastric volume and the acidity of gastric juice. It also significantly promoted gastric mucus secretion by increasing total carbohydrates and decreasing the protein concentration in aspirin+ pylorus ligated rats. The results suggest that both the extracts (ALJP and AQJP) possess antiulcer activity, whereas AQJP is more effective when compared with ALJP in aspirin+pylorus ligated rat models. The results were compared with the standard drug Rantidine, a H₂ receptor antagonist. Page(s): 181-186 2005-02-01T00:00:00Z