NOPR Collection:

Propagation of Chandipura virus in chick embryos

2005-10-01T00:00:00Z

Title: Propagation of Chandipura virus in chick embryos Authors: Pawar, S D; Singh, A; Gangodkar, S V; Rao, B L Abstract: Stocks of three Indian Chandipura virus (CHPV) isolates; one isolate from an adult febrile case in 1%5 from Chandipura town. Maharashtra, and two isolates from two pediatric encephalitis cases from Andhra Pradesh, 2003 were inoculated in 10-day-old chick embryos by allantoic route. All three virus isolates replicated in chick embryos showing titre of log 10¹² to log 10¹³ EID₅₀. The results demonstrated that chick embryos are susceptible to CHPV and virus grows to high titres in this system. Therefore chick embryos can be used as an alternative host system for cultivation and isolation of CHPV as they are less expensive than laboratory animals and have several other advantages over cell cultures. Also this system can be used for the development of vaccine and diagnostic reagent. Page(s): 930-932

Antioxidant and nitric oxide synthase activation properties of Ganoderma applanatum

2005-10-01T00:00:00Z

Title: Antioxidant and nitric oxide synthase activation properties of Ganoderma applanatum Authors: Acharya, Krishnendu; Yonzone, Parinita; Rai, Manjula; Acharya, Rupa Abstract: In vitro evaluation of antioxidant activities of Ganoderma applanatum showed significant inhibition of lipid peroxidation, and potent hydroxyl radical scavenging activity when compared with standard drug catechin. IC₅₀ values of crude, boiled and ethanolic extracts of G. applanatum were 604.8, 624 and 267 μg/ml, respectively in case of hydroxyl radical scavenging activity, and 441, 520.5 and 166.16 μg/ml, respectively in case of lipid peroxidation. Furthermore, crude, boiled and ethanoiic extracts also increased significantly nitric oxide production (156.67, 121.88 and 742 pmole/mg dry wt/hr, respectively) over the control. The results of present investigation revealed that G. applanatum have potential therapeutic use. Page(s): 926-929

Carbon source dependent somatic embryogenesis and plant regeneration in cotton, Gossypium hirsutum L. cv. SVPR2 through suspension cultures

2005-10-01T00:00:00Z

Title: Carbon source dependent somatic embryogenesis and plant regeneration in cotton, Gossypium hirsutum L. cv. SVPR2 through suspension cultures Authors: Ganesan, M; Jayabalan, N Abstract: Highly reproducible and simple protocol for cotton somatic embryogenesis is described here by using different concentrations of maltose, glucose, sucrose and fructose. Maltose (30 g/l) is the best carbon source for embryogenic callus induction and glucose (30 g/l) was suitable for induction, maturation of embryoids and plant regeneration. Creamy white embryogenic calli of hypocotyl explants were formed on medium containing MS basal salts, myo-inositol (100 mg/l), thiamine HCl (0.3 mg/l), piclorarn ((03 mg/l), Kin (0.1 mg/l) and maltose (30 g/l). During embryo induction and maturation, accelerated growth was observed in liquid medium containing NH₃NO₄ (1 g/l), picloram (2.0 mg/l), 2 ip (0.2 mg/l), Kin (0.1 mg/l) and glucose (30 g/l). Before embryoid induction, large clumps of embryogenic tissue were formed. These tissues only produced viable embryoids. Completely matured somatic embryos were germinated successfully on the medium fortified with MS salts, myo-inositol (50 mg/l), thiamine HCl (0.2 mg/l), GA₃ (0.2 mg/l), BA (1.0 mg/l) and glucose (30 g/l). Compared with earlier reports, 65% of somatic embryo germination was observed. The abnormal embryo formation was highly reduced by using glucose (30 g/l) compared to other carbon sources. The regenerated plantlets were fertile but smaller in height than the seed derived control plants. Page(s): 921-925

In vitro multiple shoot regeneration and plant production in Alysicarpus rugosus DC. var. heyneanus Baker

2005-10-01T00:00:00Z

Title: In vitro multiple shoot regeneration and plant production in Alysicarpus rugosus DC. var. heyneanus Baker Authors: Bhosle, S V; Thengane, R J; Thengane, S R Abstract: A protocol for in vitro multiple shoot regeneration and plant production through seedling (shoot tip) culture was established for Alysicarpus rngosus DC. var. heyneanus Baker. Maximum number of adventitious shoots (14.4) per shoot tip explant were initiated after two subcultures on MS solid medium supplemented with IAA (2.85 μM) plus BAP (2.22 μM) after 4 weeks. Shoot elongation (3.0-3.5 cm) was achieved on MS medium without any hormones. Stunted shoots elongated on half MS medium without growth hormones. Rooting occurred in MS medium containing IAA (1.14-2.85μM) alone or in combination with IBA (0.89-2.46μM) and or NAA (1.07-2.69μM). Maximum rooting was established in MS medium supplemented with IAA (2.85μM). The plants were acclimatized successfully with 55% survival in pot containing cocoa peat and sand (1:1). After a month, hardened plants were transferred to pots with manure, garden soil and sand (1:2:1) for further growth and finally planted in field. Page(s): 916-920