NOPR Collection:

Transmission efficiency of Culex quinquefasciatus and Aedes aegypti to Wuchereria bancrofti infection : An experimental study*

2001-01-01T00:00:00Z

Title: Transmission efficiency of Culex quinquefasciatus and Aedes aegypti to Wuchereria bancrofti infection : An experimental study* Authors: Misra-Bhattacharya, Shailja; Tyagi, K Abstract: Present study was undertaken to evaluate the suitability of natural (Culex quinquefascialus) and experimental (Aedes aegypti) vectors for supporting the development of W. bancrofti larvae for onward transmission. Both the species permitted development of W. bancrofti mf to infective larvae (L₃) within 11 to 13 days. The mf intake by both the species of mosquitoes was directly related to mf density in donor's blood. Culex exhibited higher L₃ recovery than Aedes. In Aedes maximum percent L₁ development occurred after ingesting 4 mf whereas Culex exhibited best establishment at an average mf intake of 11.5. Nevertheless wide variation in mf density in donor' s blood did not significantly affect the larval establishment in Aedes mosquito while in Culex very high (>400 mf / 40µl) or low (<50mr / 40 µl) mf counts in donor's blood adversely affected the L₃, recovery. The results reveals that A. aegrpti has an edge over the natural vector, Culex in being a voracious feeder, their easy laboratory maintenance and better transmission potential. Page(s): 98-100

Detergent-mediated destaining of Coomassie Brilliant Blue-stained SDS polyacrylamide gels

2001-01-01T00:00:00Z

Title: Detergent-mediated destaining of Coomassie Brilliant Blue-stained SDS polyacrylamide gels Authors: Pal, J.K.; Sarkar, A.; Katoch, B. Abstract: A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in distaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-mediated distaining procedure, the present method is economical and user-friendly. Page(s): 95-97

Influence of hexaconazole, carbofuran and ethion on soil microflora and dehydrogenase activities in soil and intact cell

2001-01-01T00:00:00Z

Title: Influence of hexaconazole, carbofuran and ethion on soil microflora and dehydrogenase activities in soil and intact cell Authors: Kalam, A; Mukherjee, A K Abstract: Total microbial count was highly affected (up to 61% at 1000 µg level) in presence of hexaconazole and persisted up to 21 days. Bacteria were more susceptible than actinomycetes. Carbofuran and ethion were moderately toxic to soil micronora. Inhibitory effects of all the three pesticides gradually decreased after 21 days as was evident by increase in total microbial count except in carbofuran. GDH activity in soil was also affected initially (up tol4 days) by all the three pesticides (60.3% in hexaconazole at 1000 µg level) and inhibition gradually decreased to zero except in carbofuran ( 15-20 % toxicity persisted up to 35 days). GDH and LDH activity in presence of hexaconazole was strongly affected in intact cells of some standard culture of bacteria like Rhizobium sp. (host Dolichos sp., 32 .1 and 72.5%), Bacillus subtilis Cohn (86.75 and 76.5 %), Azotobacter sp. (36.9 and 55.4%) and B.sphaericus (67.6% GDH) respectively. Carbofuran inhibited the enzyme activity in B subtilis (55.55 and 35.3%) and to some extent in B.sphaericus. Ethion moderately inhibited LDH activity in Rhodococcus sp. AK1 (17.1 and 33.3%), Rhizobium (27.6% LDH), E. coli HB 101 (34.2% LDH) as evidenced by formazan formation. From the result, it might be concluded that among the above three pesticides tested hexaconazole strongly inhibited the dehydrogenase system in bacteria including nitrogen fixing bacteria of soil and thus may affect soil fertility. It was concluded that hexaconazole was more toxic than ethion to dehydrogenase enzymes. Page(s): 90-94

Effect of temperature and relative humidity on spinning behaviour of silkworm (Bombyx mori.L)

2001-01-01T00:00:00Z

Title: Effect of temperature and relative humidity on spinning behaviour of silkworm (Bombyx mori.L) Authors: Ramachandra, Y L; Bali, Geetha; Rai, S Padmalatha Abstract: The rate of spinning of silkworm larva (Bombyx mori.L) was slow at 22°C and fast at 38°C. The time taken for completion of cocoon was longest at 98±2% and least at 40± 2% RH. However, good quality cocoons were spun at 22°C and 65±5% RH, hence it would be advantageous to maintain this temperature and relative humidity at the time of cocoon spinning in the case of CB race of silkworm. Page(s): 87-89