NOPR Collection:

Somaclonal variation studies in Orthosiphon stamineus (Benth.) using SDS-PAGE

2016-04-01T00:00:00Z

Title: Somaclonal variation studies in Orthosiphon stamineus (Benth.) using SDS-PAGE Authors: Narayani, M; Johnson, M; Babu, A; Malar, T Renisheya Joy Jeba; Janakiraman, N Abstract: The present investigation was intended to reveal somaclonal variation in Orthosiphon stamineus Benth. using SDS-PAGE as a tool. For electrophoretic studies, the proteins were isolated from mother plants, in vitro plantlets raised through nodal segments and plantlets regenerated from the leaf-derived calli of Orthosiphon stamineus, and separated using SDS-PAGE. Mother plants and nodal-segment derived plantlets were confirmed for their genetic uniformity by expressing similar banding pattern in the gel system. The proteins PP1² (0.08), PP2² (0.15), PP3² (0.25) and PP5¹ (0.41) showed their unique presence in plantlets regenerated from leaf derived calli and confirmed the occurrence of somoclonal variants. These protein profiles would be used as a biochemical marker for plant breeding or genetic improvement programme in future to identify mother plants and somaclonal variants of O. stamineus. Page(s): 269-271

Molecular evidence of Chili vein mottle virus and Chilli leaf curl virus simultaneously from naturally infected chilli plants (Capsicum annuum L.)

2016-04-01T00:00:00Z

Title: Molecular evidence of Chili vein mottle virus and Chilli leaf curl virus simultaneously from naturally infected chilli plants (Capsicum annuum L.) Authors: Sahu, Anurag Kumar; Nehra, Chitra; Mishra, Ritesh; Verma, Rakesh; Gaur, R K Abstract: The occurrence of Chilli leaf curl virus (ChLCV) and Chilli vein mottle virus (CVMV) were detected by using the duplex PCR in the mixed infected Chilli plants (Capsicum annuum L.). The duplex PCR was done by using the specific primer Pot 1 and Pot 2 for CVMV and AVF28 and AV29R for ChLCV. The amplicon and the sequence analysis confirmed the presence of potyvirus and begomovirus in the mixed infection. This combinations exhibited synergistic symptoms and large numbers of cells were doubly infected. This paper reports highly specific conventional PCR assays for detection of two independent viruses infecting chilli plants simultaneously. Page(s): 266-268

Effect of genotypes and activated charcoal on high frequency in vitro plant regeneration in sugarcane

2016-04-01T00:00:00Z

Title: Effect of genotypes and activated charcoal on high frequency in vitro plant regeneration in sugarcane Authors: Mittal, Pallavi; Devi, Ruma; Gosal, S S Abstract: A series of experiments were conducted to explore the effect of activated charcoal on the in vitro morphogenic response of 3 sugarcane varieties, viz., CoJ 83, CoJ 88 and CoJ 64. The analysis of variance at 5% level of significance indicated that the differences among different varieties and media composition containing different concentrations of activated charcoal were statistically significant. The MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 2.5 mg/L), kinetin (Kin; 0.5 mg/L) and activated charcoal (2.0 g/L) recorded maximum percentage of somatic embryogenesis in CoJ 83 (76.01), followed by CoJ 88 (72.56) and CoJ 64 (64.59). While the best plant regeneration was observed on MS medium supplemented with 6-benzylaminopurine (BAP; 0.5 mg/L) and activated charcoal (2.0 g/L) in CoJ 83 (82.32%), followed by CoJ 88 (73.35%) and CoJ 64 (69.20%). Further, maximum percentage of shoot elongation was observed in CoJ 83, followed by CoJ 88 and CoJ 64, on ½ MS medium supplemented with activated charcoal 2.0 g/L. At the same time, it was also noticed that activated charcoal promoted the shoot length in all the varieties and reduced albinism significantly in micropropagated plants. Thus activated charcoal can be added in the plant regeneration media to enhance somatic embryogenesis and plant regeneration. It can efficiently be also used to improve the frequency of shoot multiplication and transformation. Page(s): 261-265

Micropropagation prospective of cotyledonary explants of Decalepis hamiltonii Wight & Arn.—An endangered edible species

2016-04-01T00:00:00Z

Title: Micropropagation prospective of cotyledonary explants of Decalepis hamiltonii Wight & Arn.—An endangered edible species Authors: Samydurai, P; Saradha, M; Ramakrishnan, R; Kumar, S Santhosh; Thangapandian, V Abstract: The study was undertaken to standardize the development of callus, shoot and root regeneration from cotyledonary explant of Decalepis hamiltonii Wight & Arn. through the tissue culture techniques. The MS medium supplemented with 6-benzyl amino purine (BA), 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin (Kn), gibberelic acid (GA₃), indole acetic acid (IAA), indole butyric acid (IBA) and 1-naphthalene acetic acid (NAA) was used for callus, shoot and root regeneration. The maximum percentage (82.0%)of callus formation was achieved on 0.5 mg/L BA in combination with 0.05 mg/L Kn, followed by 78.5% of callus formation on 0.5 mg/L 2,4-D fortified with 0.05 mg/L Kn. The highest shoot proliferation (4.6 shoots/callus) and shoot length (6.9 cm) was achieved on 1.0 mg/L BA combined with 0.1 mg/L GA₃, followed by 3.8 shoots per callus and 5.8 cm shoot length on 1.0 mg/L IAA combined with 0.1 mg/L GA₃. The highest root formation (38.2 roots/shoot) and root length (11.8cm) was achieved on ½ strength MS medium fortified with 0.4 mg/L IBA, followed by 36.5 roots per shoot and root length of 10.7 cm on 0.4 mg/L NAA. The well-developed rooted plantlets were hardened in the mixtures of forest soil, soil and vermiculite (1:1:1) and 97.5% plantlets survived after hardening. Page(s): 256-260