NOPR Collection:

Evaluation of acute, sub-acute toxicity and cardiac activity of processed borax

2017-12-01T00:00:00Z

Title: Evaluation of acute, sub-acute toxicity and cardiac activity of processed borax Authors: Sarkar, Prasanta K.; Prajapati, Pradeep K.; Shukla, Vinay J.; Ravishankar, Basavaiah Abstract: Borax is used in Ayurveda to treat various diseases like asthma, ulcer, and others, after removal of the water for crystallisation. It causes toxicity when accumulated in the body, leads to vomiting, fatigue, and renal failure. The present study was carried out to evaluate the acute and the sub-acute toxicity and cardiac activity of processed borax in rats. Acute toxicity of processed borax was observed at the doses of 112.5, 225.0, 450.0, 900.0, and 1800.0 mg/kg. Processed borax solution at 22.5 and 112.5 mg/kg/day dose was administered for 30 consecutive days in sub-acute toxicity study. Cardiac activity of processed borax was studied by giving a borax solution at 22.5 and 112.5 mg/kg/day dose for 30 days and taking ECG. In the acute toxicity test, the processed borax did not produce any deaths. The sub-acute treatment with the processed borax (22.5 and 112.5 mg/kg, n = 6/group) failed to induce serious alterations in almost all the parameters, except toxicity in the kidney at 112.5 mg/kg. No change in ECG was observed in cardiac activity study. The LD₅₀ of processed borax is above 1.8 mg/kg. It has no serious toxicity on sub-acute exposure and has no pathological intervention on the heart in rats. Page(s): 299-305

Some potential natural dye yielding plants from the State of Goa, India

2017-12-01T00:00:00Z

Title: Some potential natural dye yielding plants from the State of Goa, India Authors: Verenkar, Nikita G. S.; Sellappan, Krishnan Abstract: Goa is a small state on the Western coast of India is gifted with more than 3,000 different species of flowering plants. Some of these plant species are known to produce the colours, thus, recognised as natural dye-yielding plants viz. Cassia fistula, Garcinia indica, Tectona grandis. During the study, natural dyes were extracted from various plant parts such as fruits, seeds, bark, flowers, roots, etc. About 62 dye-yielding plants belonging to 37 different families were recorded. Methods of extraction of dyes such as boiling the barks and grinding or chopping the leaves, fruits, flowers, etc. and various dye colours obtained are provided in this paper. These extracted dyes are showing multiple colours, the sustainable utilisation of these potential dyes may provide employment, economic and ecological benefits to the people and dyeing industries. Page(s): 306-315

Green synthesis of zinc oxide nanoparticle using Pentatropis capensis and its anti-proliferative activity

2017-12-01T00:00:00Z

Title: Green synthesis of zinc oxide nanoparticle using Pentatropis capensis and its anti-proliferative activity Authors: Surekha, K.; Abirami, R.; Prasidhee, V.; Jacob, S. Justin Packia Abstract: The ZnO nanoparticle synthesised by green synthesis method using aerial parts of Pentatropis capensis was characterised using UV-Vis spectroscopy, FTIR, SEM, and EDAX. The antiproliferative activity was tested against HepG2 and MCF-7 cell lines by MTT assay and the IC₅₀ value was calculated.The absorption peak at 330 nm confirms the presence of ZnO nanoparticle. The FTIR spectra of synthesised ZnO nanoparticle confirm the various groups present in it. The SEM image reveals the size of ZnO nanoparticle, which ranged from 73.4-398.3 nm. The elemental composition of ZnO nanoparticles consists of zinc, carbon, oxygen, potassium, calcium, and chlorine which was determined in EDAX analysis. The IC₅₀ value of anti-proliferative activity was found to be 23.277 µg/mL for HepG2 cell line and 12.926 µg/mL for the MCF-7 cell line. The green synthesised ZnO nanoparticle showed an effective anti-proliferative activity on both HepG2 and MCF cell line. Page(s): 316-321

Bioactive compounds from marine Stachybotrys sp. QL23

2017-12-01T00:00:00Z

Title: Bioactive compounds from marine Stachybotrys sp. QL23 Authors: Abdel-Razek, Ahmed S.; El-Awady, Mohamed; Hassan, Amal Z.; Yassin, Faten Y.; Asker, Mohsen; Shaaban, Mohamed Abstract: During our ongoing search for bioactive compounds from microorganisms, the marine fungus Stachybotrys sp. QL23 was isolated from a sediment sample collected from Qarun Lake, El Fayoum governorate, Egypt and biologically screened to produce bioactive compounds. The fungal extract showed moderate to high activity against Gram positive and Gram-negative bacteria assayed. The genotypic characterisation of Stachybotrys sp QL23 has been deduced basically on its 18S rRNA gene sequencing. Pre-chemical studies of the strain extract using different chromatographic techniques afforded the major bioactive metabolites, ergosterol (1) and linoleic acid (2), and their chemical structures were assigned using 1D and 2D NMR and Spectroscopy and HR-EI mass spectrometry. In this article, we present the isolation and taxonomical characterisation of the producing fungal strain, along with the study of antimicrobial activity of the strain extract and compounds 1-2 using a set of microorganisms. The study of the QL23 using cervix carcinoma cell line (KB-3-1) extract has been reported as well. Page(s): 322-328