http://nopr.niscpr.res.in/handle/123456789/54255 <b>[Pages 231-292]</b> 2026-10-12T09:07:35Z http://nopr.niscpr.res.in/handle/123456789/54263 Title: Osthole inhibits ovalbumin (OVA) induced asthma through regulating TLR9/JNK pathway Authors: Shen, Wei; Shi, Suofang; Zhang, Hui Abstract: Osthole is an active coumarin extracted from Cnidium monnieri (L.) Cusson, a traditional Chinese herb which possesses multiple pharmacological activities. Asthma is a long term disease characterized by chronic airway inflammation and hyper-responsiveness. Worldwide, around 339 million people are reported to be suffering from asthma. Coumarin osthole has been known to exhibitanti-inflammatory and antiallergic activities. Here, we investigated mechanisms underlying the protective effects of osthole in ovalbumin (OVA)induced asthmatic mice. A total 40 C57BL/6 male mice were used as experimental model animals. The results showed that osthole treatment significantly reduces OVA-induced elevated levels of serum IgE and inflammatory cytokines (IL-4, IL-5, IL-6, IL-13) except IL-10 in bronchoalveolar lavage fluid (BALF), and decreased the recruitment of inflammatory cells in BALF. The CCK-8 assay was performed to examine the viability of cells. The enzymelinked immunosorbent assay (ELISA) was used to determine the expression of inflammatory factors. Western blot analysis was conducted to examine TLR9 and JNK protein expression. Results showed that osthole significantly inhibits the recruitment of inflammatory cells and Th1/Th2 cytokines expression in BALF and airway hyper-responsiveness. Furthermore, osthole attenuated upregulation of systemic immunoglobulin production derived from OVA sensitization. Results also suggest that osthole can reverse the imbalance of Thlfrh2 in asthma, thus alleviating the symptoms of asthma to a certain extent. In conclusion, the study demonstrated that osthole, activecoumarin extract ofCnidium monnieri, attenuated the allergic airway inflammation via inhibition of TLR9/JNK pathway in mice model indicating that it could be a potential therapeutic target for asthma. Page(s): 235-241 2020-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/54262 Title: Dietary supplementation of Cinnamomumverum J. Presl and Curcuma longa L. extract on growth performance, antioxidant and metabolic enzymes activities in experimental rats Authors: Sivaranjani, R; Leela, NK; Tejpal, CS; Zachariah, T John Abstract: Cinnamomumverum J. Presl and Curcuma longa L. have many biologically active metabolites, such as sterpenoids, phenolics with proven health benefits. The present study was undertaken to analyze the effect of cinnamon and turmeric extract on growth, blood parameters, and metabolic enzyme activities of albino Wistar rats. Methanol extract of cinnamon and turmeric mixture was supplemented in rat feed at 2.5 and 5% concentration, respectively and administered for 30 days. During the course of study, body weight, feed given, feed consumed data were collected at regular intervals. After the experimental period, animals were sacrificed, tissues separated, homogenized and assayed for the activity of catalase, superoxide dismutase (SOD), glutathione-s-transferase (GST), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), alanine aminotransferase (AAT) and aspartate aminotransferase (AST). The results showed significant increase in liver catalase activity of treated animals and no significant changes in LDH, MDH, ALT and AST. No abnormalities were observed in the histopathology staining of tissue section. Overall, output of the present study could be useful for production of spices based antioxidant rich products for human health. Page(s): 242-248 2020-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/54261 Title: Cytotoxic activity of extracts of demosponges Haliclona caerulea, Axinella sinoxea and Ircinia mutans from Persian Gulf Authors: Nazemi, Melika; Ghaffari, Hadi; Moradi, Yazdan; Amiran, Mohammad Reza; Dargeri, Soheila Aghaei Abstract: Sponges are valuable source of bioactive natural products. Although marine benthic invertebrate communities occur in the Persian Gulf they have been little explored for their biomedicinal potential. Here, we studied the methanol and diethyl ether extracts of sponges Haliclona caeralea, Axinella sinoxea and Ircinia mutans sponges for their cytotoxic activity against human epidermis carcinoma (KB/C152) and T-cell lymphoma cell lines (HUT-78/C185) cell lines. Sponges after collection and identification were extracted with methanol and diethyl ether extract. The cell viability and cytotoxicity induced by the extracts were assessed using XTT and lactate dehydrogenase release assays (LDH leakage). The results indicated that the methanol and diethyl ether extract of I. mutans exhibited strong cytotoxicity towards KB and HUT cell lines and diethyl ether extract of H. caeralea and A. sinoxea had significant cytotoxicity in HUT and KB cells compared to methanol extract. The results indicated that the methanol and diethyl ether extract of I. mutanspossess significant cytotoxic activity. Page(s): 249-253 2020-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/54260 Title: Carp edema virus in ornamental fish farming in India: A potential threat to koi carps but not to co-cultured Indian major carp or goldfish Authors: Sahoo, Pramoda Kumar; Pattanayak, Sabyasachi; Paul, Anirban; Sahoo, Manoj Kumar; Kumar, Pasim Rajesh Abstract: Koi sleepy disease caused by carp edema virus (CEV) of pox virus is a potential carp killer and poses a constant threat to co-cultured fish. Only limited studies are available on its occurrence from the eastern part of India. Here, we report a large-scale mortality of koi carps (Cyprinuscarpio koi)in ornamental fish farm of Choudwar, Cuttack, Odisha. The fish showed clinical signs of ulcers on body, patchy haemorrhages, massive necrosis of gills, and observed sleeping at the bottom of tanks before death. However, the goldfish maintained in the same tanks did not show any clinical signs or mortality. We noticed Trichodina infestation in the gills of affected animals and secondary bacterium Aeromonashydrophila in kidney tissue. The gills, liver and kidneys of koi carp were found positive for carp edema virus (CEV) (Poxviridae) in PCR followed by sequencing and blast search, and phylogenetic analysis. The damages to the gills and kidney along with the presence of intracytoplasmic eosinophilic inclusions in gill epithelial cells upon histopathology suggested viral association. Experimental challenge with CEV failed to reproduce the disease in Indian major carp and goldfish, the commonly co-cultured fish species with koi carp, though, the disease was reproduced in koi carp. To our best knowledge, it is the first report of CEV infection from the farm of the eastern part of the country. Another mortality incidence in ornamental shop retailer in the same locality also revealed the presence of CEV in koi carp and pointed possibly towards its wide spread. Observations from this study suggest the need for CEV disease surveillance in the country. Page(s): 254-262 2020-04-01T00:00:00Z