http://nopr.niscpr.res.in/handle/123456789/54280 <b>Special issue on Molecular Diagnostics and Therapeutics (Guest Editor: Prof. Subir Kumar Das)</b> 2026-10-10T02:43:44Z http://nopr.niscpr.res.in/handle/123456789/54294 Title: Trianthema portulacastrum L.: Traditional medicine in healthcare and biology Authors: Das, Uttam; Saha, Tanmay; Ghosh, Rita; Das, Subir Kumar Abstract: Medicinal plants are the major folk and traditional medicine for the prevention of diseases worldwide. Trianthema portulacastrum L. (family: Aizoaceae), a small perennial weed, found in the America, Africa, India, and other regions of the world, and are extensively used not only as medicine but also as vegetable for its various health benefits. Phytochemical analysis of T. portulacastrum reveals the presence of alkaloids, phytosterols, terpenoids, saponins, flavonoids and phenolic compounds. In vitro and in vivo studies have demonstrated its pharmacological and biological activities. Different parts of T. portulacastrum L. are conventionally being used as analgesic, anti-pyretic, lipid lowering and microbicide agent; and protect liver and kidney from carcinogen, inflammation and oxidant chemicals. Page(s): 127-145 2020-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/54293 Title: Biomarkers in Overactive Bladder Authors: Pal, Manidip; Bandyopadhyay, Soma Abstract: Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), cytokines, stem cell factor etc. are some promising biomarkers for overactive bladder (OAB). To diagnose OAB urinary NGF/Creatinine (Cr) has 87.5% sensitivity and 100% specificity, and BDNF/Cr has 87.5% sensitivity and 83.3% specificity. In female OAB patient, urinary MCP-1 (monocyte chemotactic protein) was very high. In plasma 5 biomarkers IL-4 (Interleukin), TNF-α (Tumor necrosis factor), MIP-1β (macrophage inflammatory protein), SAA (Serum amyloid A), and Tie2 (Tyrosine kinase receptor) could significantly differentiate between OAB patients and control individuals. c-kit ligand, stem cell factor (SCF) found to be significantly more on overactive bladder than control. mRNA expression of connexin (Cx) and transient receptor potential (TRP) channel on urothelial cells from urine showed positive correlations of Cx26 vs urgency score, Cx40 vs nocturia, TRPM2 (Transient receptor potential melastatin 2) vs intermittency, TRPV1 (Transient receptor potential cation channel subfamily V member 1) vs urgency incontinence, and negative correlation of Cx40 vs intermittency. In plasma measurement of miR-98-5p (upregulation) + miR-139-5p (downregulation) seems to be a good biomarker (AUC = 0.839). Page(s): 146-150 2020-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/54292 Title: Thiol stabilized copper nanoparticles exert antimicrobial properties by preventing cell division in Escherichia coli Authors: Ganesh, Kumar N; Pandey, Satya Deo; Mallick, Sathi; Ghosh, Sudip Kumar; Pramanik, Panchanan; Ghosh, Anindya S Abstract: The uses of metallic nanoparticles have gained importance as one of the therapeutic options to treat infections. Here, we synthesized stable copper nanoparticles (CuNPs) using Thiosalicylic acid and assessed their antimicrobial activities against various Gram-negative bacteria. The synthesized CuNPs had absorption maxima of 570 nM with a size range of 5-11 nM and face-centred cubic (Fcc) crystal structure. The bacterial cells in their planktonic and sessile forms were susceptible to CuNPs. The nanoparticles did not show any cytotoxicity to murine macrophages (RAW264.7) below 60 µg/mL. However, the expression of oxidative stress defence gene ahpC revealed the possibility of ROS generation upon treatment with CuNPs. Interestingly, the cell division proteins like, FtsZ and FtsI were destabilized in the presence of CuNPs which in turn inhibited bacterial cell division. In conclusion, it may be stated that the synthesized CuNPs can kill bacteria by arresting cell division and/or by ROS generation. Page(s): 151-157 2020-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/54291 Title: Inflammation: A protagonist in development of carcinogen induced cervical cancer in mice Authors: Mahapatra, Elizabeth; Biswas, Souvick; Roy, Madhumita; Mukherjee, Sutapa Abstract: Inflammation- induced systemic stress plays an essential role in neoplastic progression. Chronic exposure to chemical carcinogens can induce persistent inflammatory changes which further augment loss in physiological hormesis of an organism thereby favouring carcinogenesis. The present study investigated the role of inflammation and associated systemic stress in the development of cervical carcinoma in a 3-methylcholanthrene (3-MC; a chemical carcinogen) induced in vivo cervical cancer model. When the cervix of 5-6 weeks old virgin female Swiss Albino mice (Mus musculus) was treated with 3-MC (0.6 mg/mL), remarkable alteration in its cervical cytopathology was observed. An increase in duration of 3-MC treatment caused an outburst in the number and variety of infiltrating granulocytes and agranulocytes in mice cervix. Thus, a high leukocyte index was indicative of prevalent cervical inflammatory changes. Elevated activities of SGPT, SGOT, serum alkaline phosphatase enzymes along with the presence of elevated serum creatinine levels suggested liver and renal dysfunctions. These observations were supported by alterations in hepatic histopathology of 3-MC treated mice. Surged activities and expression profiles of inflammatory cytokines (IL-6 and IL-8) in cervix tissue had conclusively established the crucial role played by inflammation- mediated systemic stress in favouring the development of cervical cancer in a carcinogen-induced in vivo model. Page(s): 158-166 2020-04-01T00:00:00Z