http://nopr.niscpr.res.in/handle/123456789/61124 2026-10-07T05:09:22Z http://nopr.niscpr.res.in/handle/123456789/62970 Title: Immature mast cells predominate in skin wound repair in senescent mice Authors: Macedo, Coelho Monique; Melo, Barreto Rafaela De; De, Resende Juliana Aparecida Pinto; Filipi, Monteiro Juan; De, Castro Rosiane Aparecida; Horta, Pinto Flávia Carmo; Rocha, Carvalho Claudia; Alves, Costa Raquel Abstract: Role of mast cells in various phases of skin wound healing is well reported in literature. However, there is much controversy about the importance of mast cells in skin wound repair. Few studies have demonstrated the presence of mast cells in skin wound repair in middle-aged and senescent individuals. The mast cells are resident cells in the skin and participate in the inflammatory process and wound repair response. It is known that there are major changes in the immune system with aging, including in the skin. In this work, we evaluated the density and maturation of mast cells (mature, intermediate and immature) at the edges of skin wounds in young, middle-aged and senescent mice by toluidine blue and alcianine-safranin blue staining. We found that there is a significant decrease in mast cells in senescent mice. There is a predominance of immature mast cells at all ages 5 days after injury. But the data are intriguing, as in senescent mice there are fewer mature mast cells. Further studies should be carried out to understand the role of mast cells during aging. Page(s): 905-910 2023-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/62969 Title: Protective effect of Lumbricus rubellus Hoffmeister extract in experimental renal ischemia/reperfusion injury in the nephrectomy rats Authors: Öz, Semih; Yıldız, Fatma; Kabay, Şahin; Şentürk, Hakan; Dönmez, Dilek Burukoğlu; Özbayer, Cansu; Üstüner, Mehmet Cengiz; Kaygısız, Bilgin; Çetin, Kısmet Çivi; Özden, Hilmi Abstract: Acute kidney injury (AKI), an immediate loss of renal function, leads to high mortality, and ischemia-reperfusion (I/R) injury is considered as one of the main causes of AKI. Inflammation and oxidative stress are known to play an important role in AKI. On the other hand, the earthworm extract, used in traditional medicine, is known to possess various biological and pharmacological activities viz. antiapoptotic, anticoagulative, fibrinolytic, anti-inflammatory, antioxidative stress, peripheral nerve regeneration, bone regeneration and wound healing. Hence, in this study, we investigated the protective effect of the earthworm Lumbricus rubellus Hoffmeister extract (LE) after nephrectomy, against oxidative stress occurring during renal ischemia/reperfusion (I/R) injury. A total of 10-12 weeks old Sprague Dawley male rats were divided into five groups (n=8). Group I (control), Group II (I/R), Group III (I/R + 10 mg/kg LE), Group IV (I/R + 20 mg/kg LE) and Group V (I/R + 40 mg/kg LE). All rats except in Gr. I were applied ischemia for 45 min and reperfusion for 24 h. At the end of the experiment, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) enzyme activities and malondialdehyde (MDA) levels were evaluated. In addition, kidney tissues were evaluated histologically. In results, the MDA and GPx level of the I/R group were found to be significantly higher than the control and LE groups. SOD activity of the control group did not differ when compared to LE groups and CAT levels were not significantly different between all groups. In addition, in Gr. III-V we observed nearly normal renal cortex and renal tubules. The present study, thus demonstrates that the extract of L. rubellus prevents renal I/R injury and induced biochemical and histological changes in the renal tissues in rats. Page(s): 911-916 2023-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/62968 Title: Molecular targets and mechanism of Longji Xiaozhong ointment in treating acute ankle sprain: A network pharmacology based analysis Authors: Li, Yumei; Zhang, Qing; Zhu, Haixia; Chen, Deta; Fan, Tianyou Abstract: Acute ankle sprain is one of the most common lower limb injuries particularly in athletes and it accounts for 16-40% of all sports-related injuries. A significant proportion of patients experience persistent residual symptoms and recurrence. Traditional Chinese medicine (TCM) offers effective treatment in terms of pain relief, swelling reduction, functional improvement, and shorter disease duration. This study provides a comprehensive analysis of the active ingredients, molecular targets, signaling pathways, and Longji Xiaozhong ointment's clinical efficacy for treating acute ankle sprains. We followed network pharmacology approach to identify the molecular targets and mechanism of action of Longji Xiaozhong ointment in treating acute ankle sprain and to validate its clinical value using relevant clinical data. Initially, Longji Xiaozhong Ointment's active ingredients were screened using the TCMSP platform based on drug properties similar to DLP ≥0.18 and oral bioavailability OB ≥30%. The molecular targets were identified from the ETCM database, followed by constructing protein-protein interaction (PPI) network diagrams and GO and pathway enrichment analysis on key genes. Furthermore, the UniProt database was used to investigate the 3D structures of these key genes. Finally, the clinical data of 111 patients with acute ankle sprains were retrospectively analyzed and further explored by comparing the different curative effects of Longji Xiaozhong ointment with Celebrex and Shexiang Analgesic ointment to check the clinical value of Longji Xiaozhong ointment in treating acute ankle sprain. In the TCMSP database, we found that there were 42 active ingredients in Longji Xiaozhong ointment, including 13 kinds of Strychnosnux-vomica L., 7 kinds of Ligusticum chuanxiong, and 22 kinds of Safflowers (Carthamus tinctorius), and a total of 3206 target genes were obtained. After applying the OB ≥50% criteriaand DL ≥0.5, 19 key target genes were selected based on their correspondence to the active ingredients. Protein mutual aid network construction and module analysis yielded two high-scoring Clusters and identified seven key proteins, including SLC6A4, ADRB2, ADRA1B, CHRM1, F2, OPRM1, and OPRD1. Functional enrichment analysis (FEA) of candidate target genes of Strychnosnux-vomica, Ligusticum chuanxiongand Safflowers in Longji Xiaozhong ointment in ETCM database showed SUMOylation of intracellular receptors, blockade of NMDA receptors, and activation of GABA A receptors, lipid metabolism regulationby peroxisome proliferator-activated receptor alpha (PPARα). PPARA activates aspects of gene expression and transcriptional activation of mitochondrial biogenesis. On retrospective analysis, all patients were divided into Group A: Celebrex combined with brace immobilization group (36 cases); Group B: Shexiang Jietong ointment combined with brace immobilization group (37 cases); and Group C: Longji Xiaozhong ointment combined with brace immobilization group (38 cases). After treatment, the VAS score, swelling degree and scope score of Longji Xiaozhong ointment combined with brace immobilization group were significantly lower than those of the other two groups, and the effect was significantly better in terms of daily activities, sports, quality of life and occurrence of re-sprain than the other two groups. Our findings, supported by clinical data, demonstrate that Longji Xiaozhong ointment effectively alleviates swelling and pain, accelerates the repair of ankle ligaments, enhances ankle joint function, and improves ankle joint stability. Page(s): 917-928 2023-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/62967 Title: Distribution of GABPB1 gene rs7181866 polymorphism in rugby players Authors: Yılmaz, Özlem Özge; Polat, Tolga; Eken, Başak Funda; Tuna, Gökhan; Aslan, Beste Tacal; Ulucan, Korkut Abstract: Nuclear respiration factor 2 is an important respiratory factor for cellular metabolism formed by polypeptides produced by the GA binding protein transcription factor subunit alpha (GABPA) and GA binding protein transcription factor subunit beta 1 (GAPPB1) genes. A functional polymorphism on the GABPB1 gene (rs7181866, G/A) alters the cellular respiration metabolism by affecting the protein dynamics. In this context, here, we examined the distribution of GABPB1 rs718186 polymorphism in professional rugby players to see its relationship with their stamina. Twelve rugby players participated in the study. Genotyping was performed by Real-Time PCR after DNAs were isolated from blood cells using commercially available isolation kits. In our cohort, the AA genotype and the A allele were superior to the AG and GG genotypes and the G allele. There was no statistically significant difference in terms of Genotypes (p=0.9027) and allelic groups (p=0.6948) in the athlete and control groups. Studies have revealed the relationship between endurance characteristics of athletes and rs7181866 AA genotype and A allele. These studies will be an important data source for future studies in sports genetics and GAPPB1 analysis. Page(s): 929-932 2023-12-01T00:00:00Z