http://nopr.niscpr.res.in/handle/123456789/6312 2026-10-10T06:22:38Z http://nopr.niscpr.res.in/handle/123456789/6371 Title: Modulation of serum cortisol by Substance P in albino rats: Evidence of a direct effect on adrenal gland Authors: Prasad, Anita; Naskar, Ranu; Dubey, Rakhi; Raha, Dipali; Ahmed, M Firoz Abstract: Effect of prolonged administration of substance P on the plasma cortisol level in the albino rats has been investigated. An inhibitory impact on intact individuals and a stimulatory effect in pharmacologically annulled rats has been observed. It is concluded that in normal conditions substance P presumably acts as a preventive agent for any excess secretion of cortisol while during stress or disturbed HPA or RAS conditions, it stimulates the secretion of cortisol. An intraglandular modulatory role of substance P has been suggested. Page(s): 163-164 2006-02-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/6370 Title: Formation of <i style="">ortho</i>-benzoquinone from sodium benzoate by <i style="">Pseudomonas mendocina</i> P₂d Authors: Parulekar, Chanda; Mavinkurve, Suneela Abstract: <i style="">Pseudomonas mendocina</i> P₂d grew in sodium benzoate at as high as 1% concentration and formed a quinonoid compound, identified as <i style="">ortho</i>-benzoquinone, that rendered the medium orange to wine-red in colour. The quinone was not metabilised further by the organism. Sodium benzoate was converted to catechol, which was a central metabolite forming <i style="">ortho-</i>benzoquinone and 2- hydroxymuconic semialdehyde (2-HMS) via.  <i style="">meta</i>ring cleavage pathway. Page(s): 157-162 2006-02-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/6369 Title: Response surface optimization of effective medium constituents for the production of alkaline protease from a newly isolated strain of <i style="">Pseudomonas</i> <i style="">aeruginosa</i> Authors: Khan, Saif; Misra, A K; Tripathi, C K M; Mishra, B N; Bihari, Vinod Abstract: Optimization of the fermentation medium for maximum alkaline protease production was carried out with a new strain of <i style="">Pseudomonas aeruginosa (B-2)</i>. Replacing the protein source/inducer (albumin in place of casein) brought about significant increase in yield after 48 hr of inoculation. Three most effective medium constituents identified by initial screening method of Plackett-Burman were albumin, (NH₄)₂SO₄ and glucose. Central Composite Design (CCD) and Response Surface Methodology (RSM) were used in the design of the experiment and in the analysis of the results. Optimum levels of the effective medium constituents were albumin (6.586%); (NH₄)₂SO₄,0.164%; and glucose, 6.72%. The alkaline protease production increased from 533460 to 793492 Ul^-1. Page(s): 151-156 2006-02-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/6368 Title: Isolation and characterization of a new osmotolerant, non-virulent <i style="">Klebsiella pneumoniae </i>strain SAP for biosynthesis of succinic acid Authors: Thakker, Chandresh; Burhanpurwala, Zainab; Rastogi, Gurdeep; Shouche, Yogesh; Ranade, Dilip Abstract: In the present study, isolation of anaerobic bacteria from 24 different eco-niches was carried out. A total number of 300 bacterial isolates, including 230 obligate and 70 facultative anaerobes were obtained using anaerobic techniques. All the isolates were initially screened for succinic acid production by Fluorescein test and TLC method. During screening, 10 isolates found to produce succinic acid were further examined by HPLC and then finally confirmed for succinic acid by LC-MS analysis. Amongst 10 isolates, isolate SAP, a facultative anaerobe isolated from buffalo rumen fluid, showed maximum yield of 2.1 g/l of succinic acid from 10 g of glucose in 24 hr under anaerobic condition. This isolate was identified as <i style="">Klebsiella pneumoniae </i>strain SAP by 16S rDNA sequence and signature sequence analysis. Mouse lethality test for the strain SAP showed LD₅₀ value of 3.3 × 10⁸ CFU/ml, which shows non-virulent nature of the strain. This strain may become a candidate strain for succinic acid production because of its osmotolerant nature and higher succinate:acetate ratio. Page(s): 142-150 2006-02-01T00:00:00Z