http://nopr.niscpr.res.in/handle/123456789/66028 2026-10-10T15:55:12Z http://nopr.niscpr.res.in/handle/123456789/66044 Title: Synthesis, DNA binding, and dual topoisomerase I/II inhibitory activities of [Ru(tpy)(adtpy)]2+ and [Ru(dtp)(adtpy)]2+ Authors: Kou, Jun-Feng Abstract: Two ruthenium (II) complexes [Ru(tpy)(adtpy)]2+ (1) and [Ru(dtp)(adtpy)]2+ (2) have been synthesized and characterized. The DNA-binding behavior of complexes have been studied by using spectroscopic and viscosity measurements. Results suggest that two of the complexes bind to DNA in an intercalative mode. Topoisomerase inhibition and DNA strand passage assay confirmed that two Ru (II) complexes act as efficient dual inhibitors of topoisomerases I and II by interference with the DNA religation. In MTT cytotoxicity studies, two Ru (II) complexes exhibit antitumor activity against BEL-7402, HeLa, MCF-7 and HepG2 tumor cells. Flow cytometry analysis shows an increase in the percentage of cells with apoptotic morphological features in the sub-G1 phase for Ru (II) complexes. Apoptosis induction has also been observed from AO/EB staining assay and Annexin V-FITC/PI double staining. Page(s): 557-570 2025-06-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/66042 Title: 2-Acetyl quinoline analogues: Synthesis, ADME analysis and molecular docking studies Authors: Satheeshkumar, Rajendran; Murugesan, Arul; Prabha, Kolandaivel; Prasad, Karnam Jayarampillai Rajendra; Sayin, Koray; Acevedo, Roberto Abstract: An efficient avenue has been developed towards the synthesis of 2-acetylquinolines from 2-aminoaryl ketones and butan- 1,2-dione using Cu(OTf)2 as a mild catalyst through Friedländer synthesis the excellent yields. The synthesized 2-acetyl-4- phenylquinoline analogues have been optimized using at B3LYP/6-31G(d) level of theory in water to calculate the contour plot of frontier molecular orbital (FMO) and molecular electrostatic potential (MEP) map. Subsequently, the biological activity of 2-acetylquinolines has been analyzed using molecular docking and ADME properties. Finally, it has been found that 3g and 3f are the best candidates for this inhibiting of EGFR. Page(s): 571-577 2025-06-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/66041 Title: Green synthesis and molinspiration analysis of novel N-Unsubstituted imide derivatives Authors: Talwan, Poonam; Gautam, Darsh; Nag, Shivam; Sharma, Mahak; Choudhary, Sahil; Nitish; Sagar; Sharma, Rishav; Pathania, Abhishek; Kholiya, Kavita Abstract: A series of novel N-unsubstituted cyclic imides have been synthesized using solvent free conditions using bentonite as a catalyst from anhydrides with urea. As a nitrogen source, urea gives the benefits of a smooth reaction, simple product separation, easy recovery, and bentonite recycling. The synthesized compounds have been analysed using TLC, FT-IR, and 1H NMR to determine their characteristics. The molinspiration online programme has been used to predict the molecular characteristics and bioactivity of the compounds. Page(s): 578-582 2025-06-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/66040 Title: Voltammetric determination of the antimalarial drug hydroxychloroquine in synthetic urine sample Authors: Sahu, Pritimala; Raghuvanshi, Nikita; Gupta, Bhanushree; Rather, Jahangir Ahmad; Singh, Namrata; Ghosh, Kallol K Abstract: The present investigation reports a rapid electrochemical determination method for an antimalarial drug, hydroxychloroquine (HCQ), using a cationic surfactant. Different solubilized media including surfactants and organic solvents have been screened, and cetyltrimethylammonium bromide (CTAB) has been selected. Electrochemical techniques, namely, Cyclic Voltammetry (CV), Square Wave Voltammetry (SWV), and Differential Pulse Voltammetry (DPV) have been employed to determine the peak current of analyte solution. Adding CTAB to the HCQ solution results in the highest peak current among the studied solubilized systems. The current signal of HCQ observed due to its oxidation is found to be a function of drug concentration, pH of analyte solution and the type of additives. The DPV results are linear over the concentration range of 5-60 μg/mL with a limit of detection at 2.41 μg/mL. Excipients including important organic compounds and amino acids have been used to study their interference in HCQ quantification. The proposed method has been successfully applied to determine HCQ in spiked synthetic urine samples. The results reveal good recovery values in the range of 89-104% for different concentrations of HCQ. Thus, the proposed method shows potential applicability for in vitro determination of the drug in urine samples. Page(s): 583-590 2025-06-01T00:00:00Z