NOPR Collection:

Optimization of some additives to improve protease production under SSF

Thu, 01 Nov 2001 00:00:00 GMT

Title: Optimization of some additives to improve protease production under SSF Authors: Tunga, Rashbehari; Banerjee, Rintu; Bhattacharyya, B C Abstract: In a locally isolated Rhizopus oryzae strain highest-production of protease (388.54/g wheat bran) was observed in presence of Tween-80 and dioctyl sodium sulfosuccinate individually at 40mg/g wheat bran concentration. Under solid state fermentation biotin (0.0025mg/g wheat bran); Ca²⁺ (0.05mg/g wheat bran) and 1-Naphthyl acetic acid (0.01mg/g wheat bran) also showed some inducing effect on the synthesis of the enzyme protease by solid state fermentation. Page(s): 1144-1148

Optimisation of fermentation conditions for gluconic acid production by a mutant of Aspergillus niger

Thu, 01 Nov 2001 00:00:00 GMT

Title: Optimisation of fermentation conditions for gluconic acid production by a mutant of Aspergillus niger Authors: Singh, Om Vir; Sharma, Amit; Singh, R P Abstract: Aspergillus niger ORS-4, isolated from the sugarcane industry waste materials was found to produce notable level of gluconic acid. From this strain, a mutant Aspergillus niger ORS-4.410 having remarkable increase in gluconic acid production was isolated and compared for fermentation properties. Among the various substrates used, glucose resulted into maximum production of gluconic acid (78.04 g/L). 12% concentration led to maximum production. Effect of spore age and inoculums level on fermentation indicated an inoculum level of 2% of the 4-7 days old spores were best suited for gluconic acid production. Maximum gluconate production could be achieved after 10-12 days of the fermentation at 30°C and at a pH of 5.5. Kinetic analysis of production indicated that growth of the mutant was favoured during initial stages of the fermentation (4-8 days) and production increased during the subsequent 8- 12 days of the fermentation. CaCO₃ and varying concentrations of different nutrients affected the production of gluconic acid. Analysis of variance for the factors evaluated the significant difference in the production levels. Page(s): 1136-1143

Effect of sublethal concentration of Bacillus thuringiensis var. kurstaki on food and developmental needs of the American bollworm, Helicoverpa armigera (Hubner)

Thu, 01 Nov 2001 00:00:00 GMT

Title: Effect of sublethal concentration of Bacillus thuringiensis var. kurstaki on food and developmental needs of the American bollworm, Helicoverpa armigera (Hubner) Authors: Gujar, G T; Kalia, V; Kumari, Archana Abstract: Effect of sublethal concentration of B.thuringiensis on the first, third, fourth and fifth instar larvae of the American bollworm, H. armigera was investigated to study their response to food consumption, digestion, utilization, and their development till adult formation. The young larvae surviving B. thuringiensis treatment in their first instar and third instar delayed larval period by two to three days, but did not consume more food as compared to control. However, they showed higher digestibility of food as compared to control, which was compensated by their reduced ability to utilize the digested food for body substance. Contrary to the effect on first and third instar larvae, the fifth instar larvae surviving B. thuringiensis treatment in its fourth instar consumed less food, showed less absorption efficiency in digesting food, but compensated by increase in the utilization of ingested and digested food into body substance. Insects surviving B. thuringiensis HD-1sublethal toxicity adapted to normal larval growth when fed on untreated food, depending upon insect growth prior to treatment. The moths emerging from B.thuringiensis treated larvae had sex ratio favouring females, and adult pairs laid less fertile eggs than those from the untreated ones. The response of B.thuringiensis treated larvae to their food and developmental needs is discussed. Page(s): 1130-1135

Allelopathic effects of Lantana camara Linn. on spore germination of Asterella angusta Steph. - A liverwort

Thu, 01 Nov 2001 00:00:00 GMT

Title: Allelopathic effects of Lantana camara Linn. on spore germination of Asterella angusta Steph. - A liverwort Authors: Kothari, Meenal; Chaudhary, B L Abstract: Extract from root, stem and leaf of L. camara proved inhibitory for germination of the spores of A. angusta. Leaf extract was found to exhibit maximum allelopathic potentiality followed by stem and root extract and may be interpreted to be the result of phytotoxic substances which are possibly synthesized in the leaf and translocated to other organs. Page(s): 1194-1198