NOPR Collection:

Duplex PCR to detect both Papaya ring spot virus and Papaya leaf curl virus simultaneously from naturally infected papaya (Carica papaya L.)

Mon, 01 Apr 2013 00:00:00 GMT

Title: Duplex PCR to detect both Papaya ring spot virus and Papaya leaf curl virus simultaneously from naturally infected papaya (Carica papaya L.) Authors: Usharani, T R; Laxmi, V; Jalali, S; Krishnareddy, M Abstract: Papaya, a major fruit crop in India and worldwide, is affected by many fungal and viral diseases. A mixed infection of Papaya ring spot virus (PRSV), a linear single-stranded (+) RNA genome of approx 10 kb size, and Papaya leaf curl virus (PaLCV), a bipartite Gemini virus (component A & B) having circular single-stranded DNA (+) genome of about 5.2 kb, has hampered the production and productivity of papaya in many parts of world. Rapid detection techniques are important in prevention of spread of the disease in field conditions. In the present study, a rapid and reliable PCR based detection protocol has been standardized. Sets of primers were designed, based on the respective virus isolate sequence data available in GenBank, to obtain anticipated products of calculated size. Page(s): 269-272

Genetic variability in Porphyra C. Ag. along the coast of Maharashtra

Mon, 01 Apr 2013 00:00:00 GMT

Title: Genetic variability in Porphyra C. Ag. along the coast of Maharashtra Authors: Kavale, M G; Chaugule, B B; Savardekar, S V; Gokhale, N B Abstract: The coast of Maharashtra (India), between Mumbai in North and Redi in South covering 720 km stretch, was visited frequently during the monsoon period for the collection of Porphyra. Morphological observations on the collected material revealed existence of morphometric diversity of greater magnitude. These populations were, therefore, subjected to molecular analysis using RAPD markers to understand the extent of genetic variability. For the study, 17 morphotypes gathered from 12 locations were used. RAPD analysis was done using 60 primers, of which 24 showed reproducible results. The primers amplified a total of 480 fragments, of which 412 were polymorphic, indicating high frequency of polymorphism. Page(s): 277-280

Regeneration of plantlets from nodal explant-derived callus cultures of Albizia lucida Benth.

Mon, 01 Apr 2013 00:00:00 GMT

Title: Regeneration of plantlets from nodal explant-derived callus cultures of Albizia lucida Benth. Authors: Saha, Dipnarayan; Kumar, Rajendra; Ramani, Ranganathan Abstract: Albizia lucida Benth. is a forest tree known for timber, gum and tannin, and lac production. Micropropagation of A. lucida can potentially address large-scale production of genetically identical superior genotypes. In the present study, a callus-mediated regeneration protocol has been established from nodal explants of A. lucida for micropropagation. Murashige and Skoog’s (MS) medium supplemented with plant growth regulators like thidiazuron (TDZ; 0.5 µM) and 6-benzylaminopurine (BAP; 2.22 mM) were found efficient in callus induction and multiplication. From the multiplied greenish and compact organogenic calli, shoots were regenerated best on MS media consisting BAP (8.88 mM), NAA (1-napthaleneacetic acid; 0.54 mM ) and AgNO₃ (5.89 mM). The excised microshoots were rooted efficiently on ½ MS medium supplemented with IBA (indole butyric acid; 9.8 mM) and BAP (0.11 mM). The rooted shoots were acclimatized in polybags containing garden soil, sand and FYM (1:1:1) and then transferred into the open. About 66.67% of the plants survived in the end. The genetic fidelity of the regenerated plantlets was found identical to the mother plant using SRAP primers. Page(s): 265-268

Impact of Al³⁺ on sludge granulation in UASB reactor

Mon, 01 Apr 2013 00:00:00 GMT

Title: Impact of Al³⁺ on sludge granulation in UASB reactor Authors: Abbasi, Tasneem; Sanjeevi, R; Anuradha, J; Abbasi, S A Abstract: Impact of trivalent aluminium (Al³⁺) ion, added to the feed of upflow anaerobic sludge blanket (UASB) reactor, was assessed on the granulation of the reactor sludge. The impact on the reactor efficiency in treating chemical oxygen demand (COD) and generating biogas was also simultaneously assessed. It was seen that, at all the three Al³⁺ concentrations (100, 200 & 300 mg/L) studied, the metal ion facilitated formation of larger-sized granules in greater proportions than the control reactor; the effect was most pronounced at 300 mg/L Al³⁺concentration. The metal fortification did not, however, influence the reactor efficiency. Page(s): 254-259

NOPR Collection:

Duplex PCR to detect both Papaya ring spot virus and Papaya leaf curl virus simultaneously from naturally infected papaya (Carica papaya L.)

Genetic variability in Porphyra C. Ag. along the coast of Maharashtra

Regeneration of plantlets from nodal explant-derived callus cultures of Albizia lucida Benth.

Impact of Al3+ on sludge granulation in UASB reactor

Impact of Al³⁺ on sludge granulation in UASB reactor