NOPR Collection:

Comparative evaluation of whole blood D-Dimer test to plasma D - Dimer test for diagnosis of disseminated intravascular coagulation

Fri, 01 Apr 2005 00:00:00 GMT

Title: Comparative evaluation of whole blood D-Dimer test to plasma D - Dimer test for diagnosis of disseminated intravascular coagulation Authors: Gupta, P K; Gupta, M; Chatterjee, T; Saxena, R Abstract: Three rapid D-dimer test methods were compared for the diagnosis of acute disseminated intravascular coagulation (DIC).These were (a) SimpliRED, an autologous red cell agglutination assay. (b) DIMERTEST latex agglutination assay, containing monoclonal antibody DD - 3B6 / 22⁶, and (c) D - DI latex agglutination assay containing mouse anti-human D-dimer monoclonal antibodies. The D-DI latex method having higher sensitivity (100%) and specificity (81%) in clinically acute DIC was postulated as the gold standard and compared with the other two methods. The results suggest that D-DI latex agglutination assay containing mouse anti-human D-Dimer monoclonal antibodies are the better assay methods amongst all the three kits analyzed. It is advisable to look for the nature of the antibody used to coat the latex particles in plasma based kits. In emergency setting RBC kits may be of some use as rapid diagnosis is advantageous. Page(s): 382-384

Regeneration of plantlets from in vitro raised leaf explants of Cleisostoma racimeferum Lindl

Fri, 01 Apr 2005 00:00:00 GMT

Title: Regeneration of plantlets from in vitro raised leaf explants of Cleisostoma racimeferum Lindl Authors: Temjensangba; Deb, Chitta Ranjan Abstract: Protocorm like bodies (PLBs), callus and shoot buds developed in culture from in vitro raised foliar explants of Cleisostoma racimeferum. Among the different basal media, better result was obtained on MS medium containing sucrose (3%) and BA (2 μM) with ~80% frequency after 40 days of culture. Young leaves (15 week old) produced better PLBs. Whole leaf placed vertically upside-up orientation can regenerate PLBs and shoot buds (80%). PLBs and shoot buds formed on entire surface of the leaves. Cultures on BA and NAA (2 and 2 μM respectively in combination) stimulated callus mediated regeneration (68%). The rooted plantlets regenerated within 8-10 week from PLBs and shoot buds on MS medium containing IAA and kinetin (2 μM each in combination). BA containing medium triggered multiple shoot bud formation, while NAA alone or in combination with other growth regulators was inhibitory. Incorporation of activated charcoal (0.01 %) in the medium stimulated formation of repetitive PLBs and multiple shoot buds. Rooted plants were ready for harvest after 20-22 week of initiation of culture. About 65% of the potted plants survived after 3 months in the poly house. Page(s): 377-381

In vitro studies of Bacopa monnieri—An important medicinal plant with reference to its biochemical variations

Fri, 01 Apr 2005 00:00:00 GMT

Title: In vitro studies of Bacopa monnieri—An important medicinal plant with reference to its biochemical variations Authors: Mohapatra, H P; Rath, S P Abstract: Micropropagation of Bacopa monnieri was achieved on MS and B5 medium supplemented with BAP and NAA using leaf explants and nodal segments. Best results were found on MS medium in both the explants with BAP (2.0 mg/l) showing higher percentage of regeneration. Besides that the biochemical parameters, like chlorophyll, carbohydrate, protein, of leaves both in vivo and in vitro have also been carried out in order to establish the sustainability of plants. Page(s): 373-376

Isolation and characterization of a green tissue-specific promoter from pigeonpea [Cajanus cajan (L.) Millsp.]

Fri, 01 Apr 2005 00:00:00 GMT

Title: Isolation and characterization of a green tissue-specific promoter from pigeonpea [Cajanus cajan (L.) Millsp.] Authors: Panguluri, S K; Sridhar, J; Jagadish, B; Sharma, P C; Kumar, P A Abstract: Expression of rbcS genes encoding small subunit of rubisco, most abundant protein in green tissue, is regulated by at least three parameters-tissue type, light conditions and stage of development. One of the green tissue-specific promoters of rbcS gene family was isolated from pigeonpea by PCR. Expression of uidA gene encoding β-glucuronidase in the transgenic tobacco plants under the control of pigeonpea rbcS promoter, clearly showed that this promoter was as strong as pea rbcS3A promoter characterized earlier. Study of the sequence similarity with pea rbcS3A promoter, especially the region (boxes I and III) that is required for rbcS3A expression, showed more than 50% divergence. In contrast, pigeonpea promoter sequence isolated in the present study was more similar to that of spinach and rice rbcS promoters. Page(s): 369-372