NOPR Collection:

Comparative protein profiles of Salmonella and Escherichia coli

Sun, 01 May 2005 00:00:00 GMT

Title: Comparative protein profiles of Salmonella and Escherichia coli Authors: Gupta, Meenal; Sharma, V D; Gupta, R S Abstract: Protein profiles of selected Salmonella serovars were compared with E. coli to identify genus specific protein(s) for Salmonella. The PDP formed of different Salmonella serovars were compared with E. coli O78 when subjected to SDS-PAGE yielded 11, 15, 15, 11 and 14 bands in S. Bareilly, S. Gallinarum, S. Typhimurium and S. Weltevreden and E. coli O78 respectively. The bands produced were compared with each other. It was found that S. Weltevreden shared 7 bands with E. coli O78, A prote in of molecular weight 20.89 kDa was found in all Salmonella serovars, but not in E. coli O78 suggesting its genus specific attribute. Page(s): 475-478

Phytopesticidal and repellent efficacy of Litsea salicifolia (Lauraceae) against Aedes aegypti and Culex quinquefasciatus.

Sun, 01 May 2005 00:00:00 GMT

Title: Phytopesticidal and repellent efficacy of Litsea salicifolia (Lauraceae) against Aedes aegypti and Culex quinquefasciatus. Authors: Phukan, S; Kalita, M C Abstract: Litsea salicifolia is one of the many plants used as phytopesticide, traditionally by various tribes of Assam. Of the five extracts of L. salicifolia tested for the bioactivity against Aedes aegypti and Culex quinquefasciatus, the aqueous extract was more effective compared to other extracts exhibiting bioactivity at 72 ppm against A. aegypti. The hexane extract (2000 ppm) exhibited 70% repellent activity for 3 hr against A.aegypti and 46% activity for 3 hr against C. quinquefasciatus. This is the first report on the insecticidal properties of L. salicifolia which can be further developed as an eco-friendly biopesticides of the future. Page(s): 472-474

Characterization of an exopolysaccharide produced by a marine Enterobacter cloacae

Sun, 01 May 2005 00:00:00 GMT

Title: Characterization of an exopolysaccharide produced by a marine Enterobacter cloacae Authors: Iyer, Anita; Mody, Kalpana; Jha, Bhavanath Abstract: An exopolysaccharide producing marine bacterium, Enterobacter cloacae, was isolated from marine sediment collected from Gujarat coast, India. Chemical investigation of exopolysaccharide (EPS 71 a) revealed that this exopolysaccharide was an acidic polysaccharide containing high amount of uronic acid, fucose and sulfate which is rare for bacterial exopolysaccharides. EPS 71a was found to have fucose, galactose, glucose and glucuronic acid in a molar ratio of 2:1:1:1. Page(s): 467-471

Molecular tagging of gene conferring leaf blight resistance using micro satellites in sorghum [Sorghum bicolor (L.) Moench]

Sun, 01 May 2005 00:00:00 GMT

Title: Molecular tagging of gene conferring leaf blight resistance using micro satellites in sorghum [Sorghum bicolor (L.) Moench] Authors: Mittal, Mukta; Boora, K S Abstract: Resistance to leaf blight in sorghum [Sorghum bicolor (L.) Moench] accession G-118 was found to segregate as a single dominant trait in a cross to susceptible cultivar, HC- 136. Molecular marker(s) linked to the locus for disease resistance was identified using simple sequence repeat (SSR) markers coupled with bulk segregant analysis. Genomic DNA from the parental cultivars and bulks were screened by PCR amplification with 50 simple sequence repeat primer pairs. Out of these, 38 SSR primers produced polymorphism between parents. After screening of these 38 SSRs with resistant and susceptible bulk, one SSR primer, Xtxp 309 produced a unique band of approximately 700 bp only in resistant parent and resistant bulk and a unique band of 450 bp only in susceptible parent and susceptible bulk. Upon screening with individual resistant and susceptible recombinant inbred lines (RILs), marker Xtxp 309 produced amplification in 23 of the 26 resistant RILs and no amplification was produced in any of the 25 susceptible RILs. The same marker Xtxp 309 produced amplification in 21 of the susceptible RILs and 3 of the resistant RILs of 450 bp band. This was found to be located at a distance of 3.12 cM away from the locus governing resistance to leaf blight which was considered to be closely linked and 7.95 cM away from the locus governing susceptibility to leaf blight. This marker may prove useful in MAS for gene introgression, plant genetic diagnostics and gene pyramiding for resistance via genetic transformation for disease resistance in plants. Page(s): 462-466