NOPR Collection:

JU-2, a novel phosphorous-containing antifungal antibiotic from Streptomyces kanamyceticus M₈

Fri, 01 Jun 2001 00:00:00 GMT

Title: JU-2, a novel phosphorous-containing antifungal antibiotic from Streptomyces kanamyceticus M₈ Authors: Datta, Indrani; Banerjee, Mila; Mukherjee, Shankar Kumar; Majumdar, Saroj Kanti Abstract: A novel phosphorous-containing antifungal antibiotic JU-2 was isolated from Streptomyces kabanyceticus M₈. Quantitative chemical analysis shows the presence of two phenylalanines, two glucose, one linoleic acid, one erucic acid and one phosphonamide moiety per molecule of the antibiotic. JU-2 shows strong inhibitory activity against various pathogenic and non-pathogenic fungi hut no activity against bacteria and yeast. Page(s): 604-606

A systemic resistance inducing antiviral protein with N-glycosidase activity from Bougainvillea xbuttiana leaves

Fri, 01 Jun 2001 00:00:00 GMT

Title: A systemic resistance inducing antiviral protein with N-glycosidase activity from Bougainvillea xbuttiana leaves Authors: Narwal, Sneh; Balasubrahmanyam, A; Sadhna, P; Kapoor, HC; Lodha, ML Abstract: An antiviral protein from Bougainvillea xbuttiana leaves induced systemic resistance in host plants N. glutinosa and Cyamopsis tetragonoloba against TMV and SRV, respectively which was reversed by actinomycin D, when applied immediately or shortly after antiviral protein treatment. When the inhibitor was applied to the host plant leaves post inoculation, it was effective if applied upto 4 h after virus infection. It also delayed the expression of symptoms in systemic hosts of TMV. The inhibitor showed characteristic N- glycosidase activity on 25S rRNA of tobacco ribosomes, suggesting that it could also be interfering with virus multiplication through ribosome-inactivation process. Page(s): 600-603

Micropropagation of Paulownia fortuneii through in vitro axillary shoot proliferation

Fri, 01 Jun 2001 00:00:00 GMT

Title: Micropropagation of Paulownia fortuneii through in vitro axillary shoot proliferation Authors: Yenkateswarlu, B; Mukhopadhyay, J; Sreenivasan, E; Kumar, V Moses Abstract: Primary cultures were established with nodal segments from juvenile shoots of two- year-old Paulownia fortuneii trees from a clonal plantation in Andhra Pradesh. A medium containing half-strength MS salts + BAP (1 mg/L) + sucrose (2%) produced optimum bud break in nodal explants. The same basal medium with reduced hormone level (0.5 mg/L) supported maximum multiplication of secondary cultures of P.fortuneii ( 1:6 in 6 weeks). Specific treatments were tested to enhance this rate of multiplication. In one approach, five to six week old in vitro grown shoots were ratooned (cutting the main shoot at the bottom leaving one node). The stumps (ratooned basal node) produced 2 to 3 axillary shoots, which grew into 4 to 5 nodes by 3 weeks; thus, providing additional shoots from the same explant. This provided 30% additional shoots in 4 cycles. Secondly, reducing the light intensity to 1200 lux resulted in higher shoot elongation, i.e, formation of 8 nodes in 5 weeks with healthier shoots than the normal intensity of 3000 lux under which only 6 nodes were produced in 6 weeks. In vitro-grown shoots could be successfully rooted ex vitro in vermiculite + cocopeat mixture ( 1: 1 v/v) under 90% humidity, transferred to soil in polybags for hardening in the green house for 2 weeks and shifted to shade net for further hardening. After one month, the plants could be success fully transplanted to field with 95% survival. Micropropagated plants showed an excellent growth in the field attaining a height of 1.5 m and a collar diameter of 2.8 cm in 3 months. Page(s): 594-599

Removal of copper by Pseudomonas putida strain S4 isolated from copper mines

Fri, 01 Jun 2001 00:00:00 GMT

Title: Removal of copper by Pseudomonas putida strain S4 isolated from copper mines Authors: Saxena, Deepa; Gowri, P. Mangala; Mago, Rohit; Srivastava, Sheela Abstract: A bacterial strain, Pseudomonas putida S4, was isolated from smelter drainage of copper mines. The strain exhibited resistance to several heavy metals, like aluminium (AI), zinc (Zn), nickel (Ni), cobalt (Co) besides copper (Cu). Strain S4 could accumulate Cu from the Cu-supplemented growth medium. In the present study, we have demonstrated the Cu²⁺removal capacity of this strain from various samples such as mine effluent, low-grade ore and ore-tailings, collected from the mining site. Moreover, ~80% of the accumulated Cu²⁺ could be recovered from the loaded biomass by a simple desorption procedure. Page(s): 590-593

NOPR Collection:

JU-2, a novel phosphorous-containing antifungal antibiotic from Streptomyces kanamyceticus M8

A systemic resistance inducing antiviral protein with N-glycosidase activity from Bougainvillea xbuttiana leaves

Micropropagation of Paulownia fortuneii through in vitro axillary shoot proliferation

Removal of copper by Pseudomonas putida strain S4 isolated from copper mines

JU-2, a novel phosphorous-containing antifungal antibiotic from Streptomyces kanamyceticus M₈