NOPR Collection:

In vitro regeneration of Hypericum patulum Thunb.-A medicinal plant

Sat, 01 Sep 2001 00:00:00 GMT

Title: In vitro regeneration of Hypericum patulum Thunb.-A medicinal plant Authors: Baruah, Anjana; Sarma, D; Saud, J.; Singh, R S Abstract: A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots. Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures. Page(s): 947-949

Somatic embryogenesis and plant regeneration in Gloriosa L.

Sat, 01 Sep 2001 00:00:00 GMT

Title: Somatic embryogenesis and plant regeneration in Gloriosa L. Authors: Jadhav, S Y; Hegde, B A Abstract: Friable callus was initiated from shoot apices of Gloriosa superba L.. on basal MS medium supplemented with 2. 4 –D (4mg L^-1) + Kn(5 mg L^-1) + CH(10 mg L^-1) + CW(20 %). Subculture of callus on the same medium after 4-5 weeks showed induction of large number of somatic embryos, which was confirmed with histological studies. Development of embryoids in plantlet took place when the embryogenic callus was transferred to basal MS medium supplemented with BAP (5 mg L^-1), CH(50 mg L^-1)+CW(20 %). Roots were developed by subculturing them on to the medium containing Kn or BAP (5 mg L^-1) and IBA (4 mg L^-1). Plantlets were successfully transferred to pots containing mixture of soil, sand and farmyard manure (2:1:1). Page(s): 943-946

Effect of probiotics on bacterial population and health status of shrimp in culture pond ecosystem

Sat, 01 Sep 2001 00:00:00 GMT

Title: Effect of probiotics on bacterial population and health status of shrimp in culture pond ecosystem Authors: Dalmin, G; Kathiresan, K; Purushothaman, A Abstract: The artificially manufactured probiotics having beneficial bacteria, Bacillus spp. was applied regularly in a modified extensive shrimp (Penaeus monodon) culture pond, located on the bank of Vellar estuary, Parangipettai. The populations of total heterotrophic bacteria (THB), beneficial bacteria (Bacillus spp.) and pathogenic bacteria (vibrios) were monitored in water and sediment of the pond. The results were compared with a control pond, situated in the same location having same water spread area, stocking density, species managed with same technologies and optimum environmental parameters in which no probiotic was applied. The populations of THB and Bacillus spp. in the experimental pond increased and the vibrios decreased after each application of probiotics. But the result of the control pond showed an increasing trend of the populations of THB, Bacillus spp. and vibrios towards days of culture. The control pond had lower levels of THB and Bacillus spp. and higher levels of vibrios than the probiotic applied (experimental) pond. Also the probiotics maintained optimum transparency and low organic load in the experimental pond as compared to control. In general, water and sediment had almost equal number of Bacillus spp. and vibrios, but sediment had higher THB load than water. The applications of probiotics lesser pathogenic vibrios and enhance beneficial bacilli in the culture leading to improved water quality, promoted growth and survival rates and increased the health status of the shrimp without stress and disease outbreaks. Thus the application of probiotics could lead to disease- free and profitable shrimp culture operations which will be helpful for shrimp farmers as most of them are now-a-days severely affected by microbial diseases. Page(s): 939-942

Primary cell culture from fish gills and kidney using fish serum

Sat, 01 Sep 2001 00:00:00 GMT

Title: Primary cell culture from fish gills and kidney using fish serum Authors: Rathore, Gaurav; Sood, Neeraj; Swaminathan, Raja Abstract: A novel, cost effective and time saving technique for primary cell culture from gills and kidney using fish serum has been developed. Single cell suspension of gills and kidney of Clarias gariepinus was obtained by trypsinization. Minimum essential medium supplemented with 10% fish serum in place of 10% foetal calf serum and 20% fish muscle extract, yielded confluent monolayer on 6th and 8th day in gill and kidney tissue respectively at 28°C. Fish serum can be successfully used as media supplement for cultivation and maintenance of primary cell culture from fishes. Page(s): 936-938