NOPR Collection:

Study on cephalopod's ink for anti-retroviral activity

Mon, 01 May 2000 00:00:00 GMT

Title: Study on cephalopod's ink for anti-retroviral activity Authors: Rajaganapathi, J; Thyagarajan, S P; Edward, J K Patterson Abstract: Aqueous extracts of ink from four cephalopods, adult and young Sepiella inermis and Loligo duvaucelli were tested against Moloney murine leukaemia virus reverse transcriptase (MML V RT). Ink from young cephalopods, S. intermis and L. duvaucelli showed strong inhibition of MMLVRT. Page(s): 519-520

Antifungal activity of bicyclic heterocyclic-1,2-diazole

Mon, 01 May 2000 00:00:00 GMT

Title: Antifungal activity of bicyclic heterocyclic-1,2-diazole Authors: Singh, Y.; Chaurasia, Leena; Nayal, S.S. Abstract: A novel series of heterocyclic-1,2 diazole namely N¹-iso nicotinoyl-5, 5'- dimethyl cyclohexane -4-(sulpha/substituted phenylazo) -1 ,2- diazoles have been synthesized. The compounds were screened for the anti-fungal properties against building fungi. The fungal species used for this purpose were Aspergillus niger and Pencillium frequentans. It was found that out of a series of 25 compounds, fourteen have shown significant fungicidal properties against both the above species. Minimum inhibition concentration was observed between 100 and 200 ppm for most of the compounds. Page(s): 516-518

Detection of tomato leaf curl geminivirus in its vector Bemisia tabaci

Mon, 01 May 2000 00:00:00 GMT

Title: Detection of tomato leaf curl geminivirus in its vector Bemisia tabaci Authors: Khan, J A Abstract: Geminiviruses are single-stranded DNA plant infecting viruses that cause major losses in important crops in tropical and subtropical countries. Tomato leaf curl virus (TLCV) belonging to the genera Begomovirus, is a whitetly-transmitted gemini virus that causes a severe leaf curl disease in tomato (Lycopersicon esculentum). The importance of this disease has prompted a great need for a rapid identification of TLCV in its hosts and vector. Polymerase chain reaction (PCR) is the most sensitive approach to detect a minute amount of viral nucleic acid. It is the most ideal method to amplify gemini viruses as they replicate via a double-stranded, circular DNA form. In this study, gemini virus specific degenerated primers were employed to detect TLCV occurring in its vector whitefly Bemisia tabaci by PCR based approach. One primer pair, amplified TLCV DNA fragment of about 1.1 Kb representing partly replicase gene, intergenic region and partly coat protein gene was used. When a set of primer targeted to the core region of the coat protein gene of gemini virus was used, a PCR amplified fragment of about 0.5 Kb was obtained. This approach is highly useful for an early detection of TLCV occurring in very small amount in the vector B. tabaci.Its implications in geminivirus management strategies and their differentiation and being discussed. Page(s): 512-515

Effect of eugenol and tincture of crataegus (TCR) on in vitro oxidation of LDL + VLDL isolated from plasma of non-insulin dependent diabetic patients

Mon, 01 May 2000 00:00:00 GMT

Title: Effect of eugenol and tincture of crataegus (TCR) on in vitro oxidation of LDL + VLDL isolated from plasma of non-insulin dependent diabetic patients Authors: Rajalakshmi, K; Gurumurthi, Prema; Devaraj, S Niranjali Abstract: The present study was carried out to study the effect of antioxidants on oxidised LDL + VLDL and found that vitamin E. eugenol and tincture of crataegus (antioxidants) inhibited oxidation of (LDL+ VLDL) similar to standard antioxidant (butylated hydroxy toluene). Vitamin C acted as an antioxidant at lower concentration, and prooxidant at higher concentration. Page(s): 509-511