NOPR Collection:

Assessment of heavy metals by ligand-less cloud point extraction in sediment and Holothuria parva (Echinodermata, Holothuroidea)

Thu, 01 May 2014 00:00:00 GMT

Title: Assessment of heavy metals by ligand-less cloud point extraction in sediment and Holothuria parva (Echinodermata, Holothuroidea) Authors: Givianrad, Mohammad-Hadi; Larijani, Kambiz; Jamili, Shahla; Adeli, Behjat Abstract: The concentrations of heavy metals were determined in organs of Holothuria parva and sediment from three stations in the Qeshm, Iran. A ligand-less Cloud Point Extraction (CPE) procedure was used for preconcentration of trace metals as pretreatment step to determination of lead and cadmium by Graphite Furnace Atomic absorption Spectrometry (GFAAS). Concentration of heavy metals in body wall, gut and sediment were obtained 14.05, 5.72 and 41.66 µg/g for lead and 0.722, 0.493 and 3.27 µg/g for cadmium, respectively. Limit of detection (LOD) values of lead and cadmium were 0.037 and 0.028 µg/g, respectively. Relative standard deviation (RSD) for six replicate determinations of lead and cadmium were 2.68% and 1.20%, respectively. Concentrations of lead and cadmium in sediments and organs of sea cucumbers differed significantly, suggesting that this sea cucumber should not be considered as an ideal bioindicator of lead and cadmium pollutants in the sediment. Page(s): 825-830

recA-based identification and antagonistic potential against fish pathogens by marine isolate DK1-SA11

Thu, 01 May 2014 00:00:00 GMT

Title: recA-based identification and antagonistic potential against fish pathogens by marine isolate DK1-SA11 Authors: Khan, Muhammad Naseem; Lin, Hong; Wang, Jingxue; Li, Meng; Mirani, Zulfiqar Ali Abstract: In this study, marine isolate DK1-SA11 was identified and analyzed for antagonistic activity against fish pathogen along with its potential possible role as probiotic agent. The strain DK1-SA11 was identified on the basis of rec-A gene sequence similarity analysis and phylogenetic studies. Strain DK1-SA11 antagonistic activity against fish pathogens was analyzed by oxford cup agar diffusion method. Acute toxicity assay of strain DK1-SA11 with zebra fish (Danio rerio) was performed to evaluate the safety of strain for its future potential application as probiotic in aquaculture. recA gene sequencing analysis identified the strain as Bacillus sbutilis subsp. spizizenii strain DK1-SA11. Furthermore, strain DK1-SA11 has shown broad antagonistic activity against fish pathogens including Aeromonas hydrophila, A. salmonicida, Vibrio alginolyticus, V. anguillarum, V. campbellii, V. chagasii, V. chagasii, Photobacterium damsela, V. diazotrophicus, V. harveyi, V. ichthyoenteri, V. mediterranei, V. mimicus, V. parahaemolyticus, V. pomeroyi, V. splendidus, V. tubiashii, V. vulniﬁcus and V. xuii. Acute toxicity results revealed that strain has neither fatal nor visible toxic effect to fish and the lethal concentration (LC₅₀) of DK1-SA11 to the fish was > ¹⁰⁸ CFU mL^-1. Therefore, it has been concluded that Bacillus subtilis subsp. spizizenii strain DK1-SA11 might be a putative strain for prevention and control of fish and shellfish diseases, and could be use for probiosis. Page(s): 731-736

Evaluation of water environmental capacity using a total optimized method — A case study of the guan river estuary in Jiangsu, China

Thu, 01 May 2014 00:00:00 GMT

Title: Evaluation of water environmental capacity using a total optimized method — A case study of the guan river estuary in Jiangsu, China Authors: Zhang, Wenhu; Li, Ruijie; Wang, Dejin; Shi, Jian; Luo, Feng Abstract: Two pollution-creating sources adjacent to the Guan River Estuary (i.e., Guan River and Xinyi River, that fed into Guan River at its mouth) were investigated in this work. A mathematical model is established for simulation analysis of the water environment of Guan River Estuary. Simulation results are verified with measured data experimentally from 12 monitoring stations in July 2007. Annual pollutant discharge of the two rivers were calculated, and the environmental capacity of Guan River Estuary was also evaluated. The dynamic relations of pollution-creating sources of the two rivers can result in the maximum amount and optimal layout of pollution discharge of Guan River and Xinyi River. Present study reveals that amounts of N and P discharged into the two rivers were substantially excessive, and adjacent water quality was below the third- and fourth-class water standard of China. Page(s): 891-896

Determination of oil and domestic pollution indicators on the southeastern Black Sea coastal water

Thu, 01 May 2014 00:00:00 GMT

Title: Determination of oil and domestic pollution indicators on the southeastern Black Sea coastal water Authors: Gedik, Kenan; Boran, Muhammet; Alkan, Nigar Abstract: Present study consists the spatial and temporal distributions of some pollutants resulting from marine and terrestrial activities were studied from the water column of the southeastern Black Sea between November 2009 and October 2010. Observed concentrations of phenol (max. 14.21 μg/l), anionic detergent (max. 28.80 μg/l), oil and grease (max. 102.90 mg/l), turbidity (max 20.20) and total suspended solids (max. 18.72 mg/l) in Black Sea water samples indicated irregular seasonal and spatial distributions. Some of the toxic pollutants exceeded the marine water quality criteria of Turkish Water Pollution and Control Regulation, suggesting significant impact of marine and terrestrial activities on the coastal waters of southeastern Black Sea. In sum, the levels of pollutants measured in this study decrease from nearshore to offshore, and the data show low variability with respect to depth. Accordingly, it can be said that the study areas are significantly affected by anthropogenic additions from wastewater discharge. Page(s): 799-804