NOPR Collection:

Micropropagation of Valeriana wallichii DC. (Indian Valerian) through nodes

Thu, 01 Jan 2015 00:00:00 GMT

Title: Micropropagation of Valeriana wallichii DC. (Indian Valerian) through nodes Authors: Singh, Sandeep; Purohit, Vijay K; Prasad, P; Nautiyal, A R Abstract: In vitro propagation method was developed for obtaining large number of plantlets of Valeriana wallichii DC. (Valerianaceae), an indigenous high value medicinal and aromatic plant species of Indian Himalayan region, using nodes of in vitro grown seedlings. High frequency shoot proliferation was induced in explants cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyladenine (BA), kinetin (Kn), adenine sulphate (AS) and thidiazuron (TDZ). Amongst all the tested cytokinins, 3.0 μM BA was found to be the most effective and an average of 12.0 shoots per nodal explant were formed after 15 d of culture. Higher concentration of TDZ (3.0 μM) was found least effective among all the cytokinins used for shoot induction. Rooting (100 %) was observed in PGR-free Murashige and Skoog medium after 12 d of incubation of microshoots. Well rooted plantlets (30-d-old) were successfully transplanted and established in mixture of soil and sand (3:1) under partially shade conditions. Page(s): 127-130

Camptothecine and methoxy camptothecine from callus cultures of Miquelia dentata Bedd.— A rare plant of the Western Ghats of India

Mon, 11 May 2015 11:27:50 GMT

Title: Camptothecine and methoxy camptothecine from callus cultures of Miquelia dentata Bedd.— A rare plant of the Western Ghats of India Authors: Thriveni, H N; Ravikanth, G; Vasudeva, R; Ganeshaiah, K N; Shaanker, R Uma Abstract: Miquelia dentata Bedd. (Family: Icacianceae) is a small climbing shrub producing anti-cancer alkaloid, camptothecine (CPT). The plant occurs sparsely in the southern parts of the forest of Western Ghats, India. Callus cultures of the plant were established using leaf explants on Murashige and Skoog medium supplemented with several plant growth regulators. The highest callus growth was obtained in the medium with 1.0 mg/L each of indole butyric acid (IBA) and benzyl adenine purine (BAP). The calli cultures produced CPT (5.4 µg/mg of the dry wt) and traces of methoxy camptothecine (MCPT). Page(s): 123-126

Development of duplex RT-PCR for detection of Konjac mosaic virus and Spathiphyllum chlorotic vein banding virus in taro and peace lily

Thu, 01 Jan 2015 00:00:00 GMT

Title: Development of duplex RT-PCR for detection of Konjac mosaic virus and Spathiphyllum chlorotic vein banding virus in taro and peace lily Authors: Manikonda, Padmavathi; Puthankalam, Srinivas Kalanghad; Masarapu, Hema; Pothur, Sreenivasulu Abstract: Konjac mosaic virus (KoMV) and Spathiphyllum chlorotic vein banding virus (SCVbV) infecting aroids in Andhra Pradesh, India have recently been reported as distinct potyviruses. Primers were designed based on their partial genome sequence (GenBank A/C EU979524, GQ421462) to detect above viruses by duplex-reverse transcription-polymerase chain reaction (duplex RT-PCR). Using this method, the two viruses were individually detected in limited number of filed/nursery collected Colocasia esculenta (taro) and Spathiphyllum spp. (peace lily). No co-infections with two viruses were traced among the screened samples. However, they were simultaneously detected in the simulated leaf samples of taro and peace lily (1:1, w/w). Page(s): 120-122

Expression analysis of BAX and GHR genes between parthenogenetic and IVF embryos in caprine

Thu, 01 Jan 2015 00:00:00 GMT

Title: Expression analysis of BAX and GHR genes between parthenogenetic and IVF embryos in caprine Authors: Singh, Renu; Kumar, Kuldeep; Kumar, Manish; Agarwal, Pranjali; Mahapatra, P S; Bag, Sadhan Abstract: The present study was carried out to compare the expression of proapoptotic (BAX) and growth hormone receptor genes (GHR) between diploid parthenogenetic and IVF (in vitro fertilization) derived preimplantation embryos in caprine. For the study, a group of oocytes were fertilized with freshly collected goat semen for production of IVF derived embryos. While another group of oocytes were activated using 7% ethanol in mSOF (modified synthetic oviductal fluid) for 5 min, followed by 4 h incubation with 2 mM 6-DMAP (6-dimethyl amino purine) in mSOF for production of diploid parthenogenetic embryos. After treatment, the oocytes were further cultured in mSOF medium for embryo development. Total RNA was isolated from morula stage diploid parthenogenetic and IVF embryos and cDNA was synthesized. The relative expression of BAX and GHR genes was analysed using real time PCR. The relative expression of BAX gene showed 18.12-fold up regulation (P˂0.05), whereas GHR gene showed 0.15-fold down regulation (P˂0.05) in diploid parthenogenetic as compared to IVF derived embryos. The present study suggests marked variation in expression of proapoptotic as well as growth related genes in parthenogenetic embryos, which might be the reason for their improper development. Page(s): 117-119