NOPR Collection:

Taxonomic and antimicrobial profiles of a rare actinomycete isolated from an inland solar saltern (India)

Sun, 01 Mar 2015 00:00:00 GMT

Title: Taxonomic and antimicrobial profiles of a rare actinomycete isolated from an inland solar saltern (India) Authors: Jose, Polpass Arul; Jebakumar, Solomon Robinson David Abstract: Rare actinomycetes from unexplored natural habitats nourish current natural compound screening programs. A rare actinomycete strain designated JAJ70; previously isolated from a hypersaline solar saltern was selected for its antimicrobial activities against bacteria and fungi. In the current study, the strain JAJ70 was studied at phenotypic and genotypic level for its proper assignment under rare genus Actinoalloteichus. Comparison of the morphological and physiological characteristics of JAJ70 with those of already known species of Actinoalloteichus showed significant divergence. Analysis of the almost complete 16S rDNA sequence of strain JAJ70 showed a similarity level ranging between 97 and 99.6% within validly reported Actinoalloteichus species. Strain JAJ70 showed significant antimicrobial activity against a range of fungal strains and clinical pathogens including Methicillin-resistant Staphylococcus aureus. In fact, the broad-spectrum activity exhibited by JAJ70 implies the need of further characterization of active antibiotic fraction from the crude extract and subsequent identification of responsible gene clusters to uncover its industrial and biotechnological significance. Page(s): 428-434

Seasonal and spatial variation of microalgal abundance and chlorophyll a concentration in intertidal rocky pools along Mumbai coast, India

Sun, 01 Mar 2015 00:00:00 GMT

Title: Seasonal and spatial variation of microalgal abundance and chlorophyll a concentration in intertidal rocky pools along Mumbai coast, India Authors: Kumar, Jeetendra; Deshmukhe, Geetanjali; Dwivedi, Alkesh; Surya, S.; Sreekanth, G. B.; Singh, Satyendra Kumar Abstract: Microalgal recruitment, density and productivity in term of chlorophyll a in intertidal areas of rocky coast along Mumbai were estimated. Diatoms was dominant contributing maximum chlorophyll a in studied area. Thalassiosira spp., Nitzschia spp. and Navicula spp. were dominant microalgae. Microalgae was significantly positive correlated with SST (p<0.01), DO (p<0.01), Chl a (p<0.05), diatoms (p<0.01) and dinoflagellates (p<0.01) and significantly negative correlated with nitrate (p<0.05). Chl a was significantly positive correlated with SST (p<0.05), nitrate (p<0.01) and diatoms (p<0.01) and significantly negative correlated with euglenozoa (p<0.05), phosphate (p<0.05), salinity (p<0.05) and pH (p<0.01). Phosphate and nitrate significantly negative correlated (p<0.01). Shannon’s index, Simpson’s index, Evenness and Equitability were recorded higher in pre-monsoon season while dominance (D) was highest in post monsoon season. Page(s): 421-427

Antibacterial activity of Chaetomorpha litorea (harvey) against isolated fish bacteria

Sun, 01 Mar 2015 00:00:00 GMT

Title: Antibacterial activity of Chaetomorpha litorea (harvey) against isolated fish bacteria Authors: Haniya, Abdul Majeeth Kamarul; Sweety, Francis Youthtith; Kothai, Seshathri; Mahalakshmi, Karuppiah Abstract: In the present study the methanolic extract of Chaetomorpha litorea (Harvey) was screened for antibacterial activity against the bacterial strains isolated from fish. Antibacterial effect of C. litorea was studied by Minimal Inhibitory Concentration (MIC) method. Result of MIC showed that the methanolic extract of Chaetomorpha litorea expressed its antibacterial activity against isolated fish bacterial strains at lower concentration (50 to 1.562 µg/ml). In order to identify the active principle rendering the antibacterial effect of Chaetomorpha litorea, the preliminary phytochemical screening, HPLC and FT-IR analysis were done. HPLC spectrum showed 19 peaks (1 major and 18 minor), indicating the presence of nineteen components and the FT-IR spectrum indicates the presence of compound with OH and C-H group. Page(s): 416-420

Studies on the effect of Artemia franciscana on the removal of Chromium by Bioaccumulation

Sun, 01 Mar 2015 00:00:00 GMT

Title: Studies on the effect of Artemia franciscana on the removal of Chromium by Bioaccumulation Authors: Devi, Selvaraj Sujatha; Sethu, Mathialagan; Priya, Ponnaiah Gomathi Abstract: In the present work, the bioaccumulation of Cr (VI) by Artemia franciscana was investigated. Metallothionein protein plays a key role in the uptake of Cr (VI) by Artemia and it was estimated by the silver saturation method. Artemia (Brine Shrimp) was subjected to Cr (VI) initial concentration of 40 ppm which was reduced to 2 ppm on 4^th day of experimental condition with removal efficiencies of 95%. The number of organisms were varied to find out the maximum removal efficiency. Physical parameters such as pH were maintained in alkaline condition of 9-10, temperature was maintained at room temperature and 30-35 ppt of salinity condition was found to be optimum for the survival and reduction of chromium by Artemia. Page(s): 411-415