http://nopr.niscpr.res.in/handle/123456789/39729 Sun, 11 Oct 2026 16:52:00 GMT 2026-10-11T16:52:00Z http://nopr.niscpr.res.in/handle/123456789/39746 Title: DNA binding, cytotoxicity and mitochondria-mediated cell apoptosis induction of a new zinc(II) complex of 5-chloro-8-hydroxylquinoline Authors: Zhang, Hai-Rong; Meng, Ting; Qin, Qi-Pin; Deng, Sheng-Ping; Cao, Qian-Qian; Han, Hong-Hua; Li, Ying-Bo; Zhang, Yun-Liang; Liu, Yan-Cheng; Liang, Hong Abstract: A new zinc(II) complex (<strong>1</strong>) of 5-chloro-8-hydroxylquinoline (HClQ) has been synthesized and characterized by IR spectroscopy, ESI-MS spectrometry, elemental analysis and single crystal X-ray diffraction analysis. The DNA binding property of complex (<strong>1</strong>) has been investigated by UV-vis spectroscopy, circular dichroism spectroscopy and agarose gel electrophoresis. The results indicate that complex (<strong>1</strong>) binds with ct-DNA via an intercalative mode, and also exhibits DNA cleavage activity. The <em>in vitro</em> cytotoxicity of complex (<strong>1</strong>) has been screened by MTT assay against a series of tumor cell lines as well as HL-7702 normal liver cell line and, compared with that of cisplatin. Complex (<strong>1</strong>) shows lower cytotoxicity than cisplatin towards both the tumor cell lines and the normal liver cell line, except the BEL-7404 liver tumor cell line. The IC₅₀ value of (<strong>1</strong>) towards BEL-7404 is 7.04&plusmn;0.06 &mu;<em>M</em>, which is lower than that of cisplatin (25.08&plusmn;0.12 &mu;<em>M</em>). This suggests that BEL-7404 is the most sensitive tumor cell line for (<strong>1</strong>). Complex (<strong>1</strong>) is found to induce cell apoptosis in the BEL-7404 cells by arresting the cell cycle at the S phase. The antitumor mechanism of (<strong>1</strong>) involves targeting the mitochondria-mediated pathway, since the ROS release and the cytoplasmic [Ca²⁺]_c increase in the tested tumor cells after incubation with (<strong>1</strong>). Flow cytometry assay on the cellular level confirms that the dysfunction of the mitochondria (indicated by the loss of mitochondrial membrane potential) and the release of ROS and Ca²⁺, directly cause the activation of caspase cascade, including caspase-9 as the initiator and caspase-3 as the executor. These results strongly suggest that the central Zn(II) as the coordinating centre plays the key role in enhancing the antitumor activity and actuating the potential apoptotic pathway for this kind of halogenated quinoline derivatives. Page(s): 1433-1442 Thu, 01 Dec 2016 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/39746 2016-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/39741 Title: Investigations on the binding behavior of competitive ions to a cationic micelle by ¹H -NMR studies Authors: Sultana, Nazima Abstract: The binding nature of aliphatic carboxylate ions, viz. acetate, propionate and butyrate, to a cationic micellar surfactant, viz. cetylpyridinium chloride, has been investigated by ¹H-NMR spectroscopy. The carboxylate ions are expected to compete with the chloride ion for binding at micellar interface due to the specific ion effect. The counterions, Ac^-, Pr^- and Bu^-, bind to the cetylpyridinium micelle such that the distance of COO^- group from the head group of CPC is almost the same for all the three counterions. This implies that as the length of the alkyl chain of the aliphatic carboxylate counterion increases, it stretches deeper into the palisade layer or core of the micelle. Furthermore, the preference of one ion over another for binding at biological interfaces is broadly termed as specific ion effect and micelles are used as models to mimic the biological membrane. Therefore, effect of competitive ions on the micellization of ionic surfactants provides useful information to better understand the effect of specific ions. Page(s): 1443-1448 Thu, 01 Dec 2016 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/39741 2016-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/39737 Title: Catalytic oxidation of cyclohexene by α-zirconium phosphate intercalated Mn(Salen) using 70% <em>tert-</em>butylhydroperoxide as an oxidant Authors: Khare, S; Chokhare, R; Shrivastava, P; Kirar, J S; Parashar, S Abstract: A heterogeneous catalyst, α-zirconium phosphate intercalated Mn(Salen) complex, abbreviated as {α-ZrP.Mn(Salen)} has been synthesized by the flexible ligand method. The resulting compound is characterized by BET surface area, X-ray diffraction, energy dispersive X-ray analysis, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and atomic absorption spectroscopy. The catalytic activity of α-ZrP.Mn(Salen) has been tested for the oxidation of cyclohexene using 70% <em>tert</em>-butylhydroperoxide as an oxidant. In the oxidation reaction, cyclohexene is oxidized to cyclohexene oxide, cyclohexenol and cyclohexenone. Influence of various reaction parameters, viz., reaction temperature, catalyst concentration, substrate concentration and oxidant concentration are studied. Under the optimized conditions, oxidation of cyclohexene gives a maximum of 85.50% conversion. The major order of selectivity of the product is: cyclohexenone > cyclohexenol > cyclohexene oxide. The catalyst can be reused for three cycles without significant loss of catalytic activity. The heterogeneity of the catalyst has been tested by hot filtration experiment. Page(s): 1449-1457 Thu, 01 Dec 2016 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/39737 2016-12-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/39736 Title: Determination of the azo dye, sunset yellow, using carbon paste electrode modified with molecularly imprinted polymer Authors: Wang, Zhihua; Shan, Yijiang; Xu, Lijuang; Wu, Guofan; Lu, Xiaoquan Abstract: A highly selective voltammetric sensor for sunset yellow is reported. Sunset yellow selective molecular imprinted polymers (MIP) and non-imprinted polymers have been synthesized and used for preparation of carbon paste electrode. The MIP, incorporated in the carbon paste electrode, functions as selective recognition element and pre-concentrator agent for determination of the dye. The sensitivity of the sensor is improved greatly because of the multiwalled carbon nanotubes (MWCNTs). The complete removal of the dye from the polymer was confirmed by the infrared spectra and the differential pulse voltammetry. The MWCNTs/MIP/CPE has been characterized by electrochemical impedance spectroscopy, cyclic voltammetry and DPV. The MWCNTs/MIP/CPE shows very high recognition ability in comparison to the MWCNTs/NIP/CPE. Under the optimum conditions, the linear relationship between current and concentration was obtained in the range from 8&times;10^-8 to 1&times;10^-5mol L^-1. The detection limit of the sensor is 2.667&times;10^-8mol L^-1(S/N=3). Page(s): 1458-1464 Thu, 01 Dec 2016 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/39736 2016-12-01T00:00:00Z