NOPR Collection:

Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles

Sat, 01 Nov 2008 00:00:00 GMT

Title: Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles Authors: Hema, M; Reddy, Ch V Subba; Savithri, H S; Sreenivasulu, P Abstract: Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization. Page(s): 793-796

Efficacy of 4-methyl-7-hydroxy coumarin derivatives against vectors Aedes aegypti and Culex quinquefasciatus

Sat, 01 Nov 2008 00:00:00 GMT

Title: Efficacy of 4-methyl-7-hydroxy coumarin derivatives against vectors Aedes aegypti and Culex quinquefasciatus Authors: Deshmukh, Madhavi; Pawar, Pushpa; Joseph, Mary; Phalgune, Usha; Kashalkar, Rajashree; Deshpande, Nirmala R Abstract: 4-Methyl-7-hydroxy coumarin is considered as a lead molecule as a biopesticide. Its mono bromo and tribromo derivatives were synthesized. Two more derivatives were synthesized by acylation. Compound 1 (3,6,8-tribromo-7-hydroxy-4-methyl-chromen-2-one) was found to be the most potent against IV^th instar larvae of C. quinquefasciatus and A. aegypti the LC₅₀ being 1.49 and 2.23 ppm respectively. It showed 100% larval mortality at 25 ppm against A. aegypti and at 10 ppm against C. quinquefasciatus. Compounds 1 and 2 (3,6,8-tribromo-7-hydroxy-4-methyl-chromen-2’-oxo-2H-chromen-7-yl acetate) showed remarkable ovicidal activity. Significant reduction of 80-85% hatching of eggs of both mosquito species was observed at the highest dose of 100 ppm. The hatched larvae showed 100% mortality in the successive instars. Compounds 3 and 4 (3-bromo-7-hydroxy-4-methyl-chromen-2-one and 3-bromo-4-methyl-2’-oxo-2H-chromen-7-yl acetate) showed moderate activity against both mosquito species. Page(s): 788-792

Screening of natural phenolic compounds for potential to inhibit bacterial cell division protein FtsZ

Sat, 01 Nov 2008 00:00:00 GMT

Title: Screening of natural phenolic compounds for potential to inhibit bacterial cell division protein FtsZ Authors: Rastogi, Neerja; Domadia, Prerna; Shetty, Sangeeta; Dasgupta, Debjani Abstract: FtsZ plays an important role in bacterial cell division by polymerizing to form the Z ring at the site of cytokinesis. Phytochemicals are known to disrupt bacterial cell division through inhibition of FtsZ assembly. In the present study phytochemicals like eugenol, trans-cinnamic acid, 4-formyl cinnamic acid, naringenin and caffeic acid were were tested for their potential to inhibit cell division. Effect of these antimicrobial compounds on the growth of E. coli was determined and the inhibition of FtsZ assembly in vitro was investigated. The present study revealed trans-cinnamic acid as the most potent inhibitor of FtsZ assembly. Page(s): 783-787

Wound healing activity of Sesamum indicum L seed and oil in rats

Sat, 01 Nov 2008 00:00:00 GMT

Title: Wound healing activity of Sesamum indicum L seed and oil in rats Authors: Kiran, Kotade; Asad, Mohammed Abstract: The seeds of S. indicum L (Pedaliaceae) are used traditionally in the folklore for the treatment of various kinds of wounds. The present study was undertaken to verify the effect of S. indicum seeds and its oil on experimentally induced excision wound, incision wound, burn wound and dead space wound models in rats. Aloe vera was used as standard wound healing agent. A formulation of seeds and oil was prepared in carbopol at 2.5% and 5% concentrations and applied to the wounds. In the excision and burn wound models, the so treated animals showed significant reduction in period of epithelization and wound contraction (50%). In the incision wound model a significant increase in the breaking strength was observed. Seeds and oil treatment (250 mg and 500 mg/kg; po) in dead space wound model, produced a significant increase in the breaking strength, dry weight and hydroxyproline content of the granulation tissue. The results suggest that S. indicum seeds and oil applied topically or administered orally possesses wound healing activity. Page(s): 777-782