NOPR Collection:

Cloning and analysis of promoter elements of a Ser/Thr protein kinase gene homologue from Piper colubrinum Link.

Wed, 01 Jan 2020 00:00:00 GMT

Title: Cloning and analysis of promoter elements of a Ser/Thr protein kinase gene homologue from Piper colubrinum Link. Authors: Mani, Tomson; Manjula, S. Abstract: Serine/Threionine (Ser/Thr) protein kinases are multifarious phosphorylation enzymes which also play a central role in signalling events following pathogen recognition in plants. The present study describes cloning and characterisation of upstream promoter region of a Ser/Thr protein kinase (STPK) gene from Piper colubrinum (PcSTPK), a wild species highly resistant to fungal pathogens in the Piper germplasm. The gene was found to be pathogen responsive when challenged with oomycete pathogen – Phytophthora capsici. Transcript abundance of PcSTPK was determined by quantitative real time-polymerase chain reaction (qRT-PCR) which demonstrated maximum transcript accumulation of PcSTPK in inflorescence, followed by leaf, stem and root tissues. Inoculation of leaf tissues with the oomycete pathogen Phytophthora capsici, also induced significant transcript accumulation of PcSTPK in the plant. Genome walking methodology was adopted to clone upstream promoter elements of PcSTPK. In silico analysis revealed the presence of regulatory elements for light responsiveness, meristem and endosperm expression in addition to various hormone responsive elements. Our results suggest that PcSTPK along with its cis-regulatory elements has a role in modulation of plant stress response. Page(s): 9-16

Seed storage protein phylogenetics of Indian wheat genotypes belong to Triticum aestivum, T. dicoccum and T. durum

Wed, 01 Jan 2020 00:00:00 GMT

Title: Seed storage protein phylogenetics of Indian wheat genotypes belong to Triticum aestivum, T. dicoccum and T. durum Authors: Sihmar, Monika; Sharma, Jitendra Kumar; Santal, Anita Rani; Singh, N. P. Abstract: Genetic diversity analysis of eighty six Indian wheat genotypes was performed using sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) electrophoresis based on their total seed storage protein. A dendrogram was constructed using the genetic similarity coefficient matrix based on SDS-PAGE, which categorized these genotypes according to their electrophoretic patterns into eight different clusters. These eighty six genotypes studied here belong to three different species of wheat. Out of these eighty six genotypes, two genotypes belong to T. dicoccum; eleven belong to T. durum and the remaining seventy three belongs to T. aestivum. In the dendrogram, all the eleven genotypes of the species T. durum (A-9-30-1, HD-4672, RAJ-1555, PDW-233, PBW-34, HI-8381, PDW-291, WH-896, PDW-215, HI-8498 and MACS-2846) were grouped in one cluster. The pedigree analysis of genotypes showed similar parentage with the electrophoretic profiling of the different wheat genotypes. From this study, it has been concluded that the total seed storage protein profile of wheat can be used as a marker for genetic diversity. Page(s): 17-27

Identification of urease producing Virgibacillus sp. UR1 from marine sediments

Wed, 01 Jan 2020 00:00:00 GMT

Title: Identification of urease producing Virgibacillus sp. UR1 from marine sediments Authors: Sarkar, Gargi; Suthindhiran, K Abstract: Urease producing bacteria have been reported from various ecosystems including soil, water and host organisms such as humans and animals. This research describes the isolation and identification of ureolytic bacteria from calcium rich marine sediments of southern India. The potential strain was designated as UR1 and identified using polyphasic taxonomy. The results show that the strain belongs to the genus Virgibacillus and is closely related to the genus Bacillus. Further, the urease was extracted by 80% acetone precipitation method and the molecular weight was determined as ~80 kDA by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The high performance liquid chromatography (HPLC) analysis of the urease in comparison with standard indicates that the isolated enzyme is urease. The enzyme exhibited the maximum activity at the temperature of 45^oC and pH of 9. Furthermore, the crude enzyme exhibited notable calcite precipitation signifying that the strain could be used for microbial induced calcite precipitation (MICP) and subsequently for biocementation process. Page(s): 28-37

Antibacterial and fibrinolytic potential of Himalayan soft gold mushroom Cordyceps sinensis

Wed, 01 Jan 2020 00:00:00 GMT

Title: Antibacterial and fibrinolytic potential of Himalayan soft gold mushroom Cordyceps sinensis Authors: Agrawal, Diva Gupta; Sandhu, Sardul Singh Abstract: The medicinal mushroom Cordyceps sinensis collected from Himalayan region of India was found to have bioactive compounds. The mycelia free culture filtrate obtained from fermentation of Cordyceps mycelia were partially purified by solvent-solvent extractions. The subsequent fractions were tested for their potential regarding antibacterial and fibrinolytic activity. The butanolic fraction and aqueous layer showed significant antibacterial activity against six bacterial strains.While the final aqueous layer and hexane fraction showed partial fibrin lytic capacity in comparison with commercially available streptokinase as a positive control. It was assumed that the metabolite fractions when purified further can act as good antibacterial and fibrinolytic agents. Page(s): 38-44