http://nopr.niscpr.res.in/handle/123456789/60317 <b>Special issue on “Marine Natural Products”</b> Sat, 10 Oct 2026 18:06:41 GMT 2026-10-10T18:06:41Z https://http://nopr.niscpr.res.in:443/retrieve/185465/IJMS May 2022.png http://nopr.niscpr.res.in/handle/123456789/60317 http://nopr.niscpr.res.in/handle/123456789/60334 Title: In-silico analysis of bioactive compounds extracted from seaweed Amphiroa anceps on the pathogenicity of bacteria Authors: Madathil, D; Chidambaram, R Abstract: Screening of biochemical compounds in marine algae is a gateway to understand the bioactive principles of antimicrobial activity and may be a significant tool in developing environment friendly herbicides and in drug discovery. In this study, the antibacterial activity of the seaweed Amphiroa anceps extracted with methanol and ethanol was determined against clinical isolates namely Vibrio cholerae 01 ogawa, Vibrio cholerae O139, Vibrio fluvialis, Vibrio parahaemolyticus, V. parahaemolyticus 81, V. parahaemolyticus O3:K6, salmonella enteritidis type 5, Enterotoxigenic E. coli, Bacillus cereus by disc diffusion method at different concentrations. Ethanolic extract showed the highest activity against V. parahaemolyticus, V. parahaemolyticus 81 and V. cholerae. The FTIR results showed the presence of major functional groups like amine, hydrogen bonded alcohols, alkanes and aromatic rings. Four major bioactive compounds were selected based on GC-MS data and used as ligands to dock in-silico against proteins of pathogenic bacteria that were used in antibiotic studies. Page(s): 379-387 Sun, 01 May 2022 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/60334 2022-05-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/60333 Title: In-vitro screening of amylase producing halophilic bacteria isolated from seawater Authors: Abirami, S; Lakshmi, M Muthu; Samrot, A V; Cypriyana, P J Jane; Saigeetha, S; Narendrakumar, G Abstract: In this study, eight halobacterial strains were isolated from seawater. Among the eight isolates, four strains grew well in nutrient medium with 40 g/100 mL of sodium chloride concentration. Amongst all, HA01 was found to show highest amylase activity than others. Highest amylase producing bacteria, i.e. HA01 was subjected for 16S rRNA sequence based identification and it was found to be Aquabacterium sp. strain AS02. The organism showed optimal amylase production at pH 7 and temperature of 42 °C and NaCl concentration of 3 M. The two substrates (banana peel and potato peel) were used for the amylase production among which banana peel showed that highest amylase activity of 6.12 U/mL than potato peel (4.27 U/mL). Page(s): 388-395 Sun, 01 May 2022 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/60333 2022-05-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/60332 Title: In-vitro biocompatibility studies of nanochitosan/ polyvinylpyrrolidone blends on MC3T3-E1 cells showing beneficial effect in bone tissue engineering Authors: Sangeetha, V; Sudha, P N; Jayaprabakar, J Abstract: Chitosan is a naturally occurring polymer with numerous biological properties that can be used in the biomedical field. Chitosan was modified to nanochitosan in this study. The polycationic nature of chitosan interacts with polyanions (TPP) to form nanoparticles. The prepared nanochitosan was mixed with polyvinyl pyrollidone to create binary blends with ratios of 1:1, 1:2, and 2:1. The polymeric blends were interpreted by using the analytical tools FTIR, XRD and SEM. The results of the FTIR and XRD confirm the formation of binary blends with strong electrostatic interaction. The study aims to investigate the compatibility of the prepared blend as tissue regenerative material. The blends were evaluated in-vitro using fluorscent spectroscopy, MTT assay, NRR assay and ALP assay to study its biocompatibility and cell viability. The results revealed that the quick multiplication followed by a moderate increment in cell number of MC3T3-E1 as cells reacted to the prepared material and hence the materials can be used in the regenerative medicine. Page(s): 396-403 Sun, 01 May 2022 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/60332 2022-05-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/60331 Title: Analysis of bioactive compounds from Gracilaria foliifera based on lunar phases Authors: Abraham, L S; Prakash, P; Sai Krishna, N M D; Sunkar, S; Paramasivam, O R; Thomson, B I; Rajakumari, K Abstract: Gracilaria foliifera, sustainable renewable resources in the marine environment. Gracilaria, a genus of red algae, notable for its economic importance as an agarophyte. In the present study, experiments were performed to investigate the phytochemical constituents of Gracilaria foliifera. Samples were collected during three different lunar phases namely new moon, full moon and between days. The collected seaweeds were shade dried and extracted by ethyl acetate. The crude metabolites are subjected to phytochemical analysis, antioxidant activity, and qualitative analysis of the compounds by TLC. Further the crude extract was evaluated by GCMS. Among the different lunar phases, the presence of phytochemical compounds, antioxidants activity, is maximum during the full moon days which also showed appreciable amount than the samples collected during new moon phase and transition phase. Page(s): 404-411 Sun, 01 May 2022 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/60331 2022-05-01T00:00:00Z