http://nopr.niscpr.res.in/handle/123456789/64429 Sat, 10 Oct 2026 19:06:23 GMT 2026-10-10T19:06:23Z https://http://nopr.niscpr.res.in:443/retrieve/193367/Cover.png http://nopr.niscpr.res.in/handle/123456789/64429 http://nopr.niscpr.res.in/handle/123456789/64439 Title: Optimization of HPTLC densitometric method for curcuminoids and polyphenolics in an ayurvedic Emblica officinalis and Curcuma longa based Nishamalaki formulation by Box-Behnken design Authors: C, Patel Kruti; G, Patel Kalpana; Aboli, Girme; Ganesh, Saste; R, Parmar Rajesh; Lal, Hingorani Abstract: The present study focuses on developing a simplified, specific, and accurate high performance thin layer chromatographic (HPTLC) method for the quantitative and qualitative determination of ellagic acid, gallic acid, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) in Nishamalaki Ayurvedic formulation. Pre-coated silica gel 60 F254 aluminum-backed plates were used as the stationary phase in the chromatographic technique development, and the optimized mobile phase was toluene: dichloromethane: glacial acetic acid: formic acid (6:4:1.6:0.9% v/v/v/v) with double development in linear ascending mode. The detection wavelength for quantification for ellagic and gallic acid was 280 nm, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) were 430 nm. The optimized mobile phase showed optimum separation between peaks for ellagic acid, gallic acid, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) at RF of 0.12±0.02, 0.21±0.02, 0.55±0.02, 0.69±0.02 and 0.82±0.02 respectively. Chromatographic conditions were optimized using the Box-Behnken design. Various variables, such as, the volume of formic acid and glacial acetic acid, and chamber saturation time, that are likely to impact RF were identified for further optimization. The volume of glacial acetic acid may be regarded as a critical method parameter, which caused the greatest change in the RF value and was the important factor among the three factors. The linear range was 600-1800 ng/band for all markers (r2 greater than 0.98). The limit of detection (LOD) and quantification (LOQ) measured indicated the method’s sensitivity. For all markers, the recovery percentage reveal acceptable accuracy, and the method was repeatable and reproducible from precision measurements with less than a 2% relative standard deviation. The optimized method was precise, specific, accurate, robust and reproducible for quantifying ellagic acid, gallic acid, and curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) in the quality-control testing of botanical extract along with Nishamalaki ayurvedic formulation. Page(s): 719-728 Thu, 01 Aug 2024 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/64439 2024-08-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/64438 Title: Clinical efficacy of Gojihvadi Kwath, Shirishadi Kwath, Sanjeevani Vati, Panchagavya Ghrita Granules & Shunthi Churna for COVID-19 management, a randomized controlled trial Authors: Byadgi, Parameswarappa S; Kumari, Meena; Singh, Vishwambhar; Dwivedi, Arun Kumar; Mishra, Sunil Kumar; Dubey, Sushil Kumar; Chaudhary, Ashvanee Kumar; Chaurasia, R N; Joshi, Namrata; Jani, Hitesh; Mishra, Rajeev Kumar; Singh, T B; P S, Arunima; Jayakumar, Remya Abstract: The obscure disease pattern of COVID -19 have to be evaluated with Ayurvedic interventions and the present clinical trial was a randomized open label parallel three-arm control trial on the major and minor symptomatic patients with the recent pandemic. The patients were recruited from the Super specialty building of Sir Sunderlal hospital, COVID- 19 ward and home isolated ones. The Ayurvedic intervention includes the 15 days oral administration (Group A) –Gojihvadi Kwath, Sanjeevani Vati; Panchagavya Ghrit Granules; Shunthi (Dry ginger powder) plus conventional treatment, in (Group B) –Shirishadi Kwath, Sanjeevani Vati; Panchagavya Ghrit Granules; Shunthi (Dry ginger powder) plus conventional treatment and in Group C (control group) only the conventional medicines. Assessment was done based on the RT-PCR reports, signs and symptoms. The results point out the efficacy of trial medicine (that is group A and B) to discharge the patients earlier than those from the control group. The early reduction in the signs and symptoms noted were also observed. Group B showed a faster recovery from dry cough and shortness of breath and improvement in appetite. Home-isolation patients showed faster recovery of clinical symptoms than the hospitalized patients. Ayurvedic interventions can accelerate viral load clearance associated with quicker recovery and concurrently decrease the risk of viral dissemination. Additionally, there were no adverse reactions observed with these trial medicines. Page(s): 729-738 Thu, 01 Aug 2024 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/64438 2024-08-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/64437 Title: Evaluation of toxic heavy metal content in marketed Ayurvedic decoctions using closed vessel microwave digestion in ICPMS Authors: Mohanan, Deepu; Puthiyedath, Rammanohar; G, Nandakumar; G, Minsha M; S, Sushma N; K, Shyamasundaran Abstract: The heavy metal toxicity of marketed Ayurvedic herbal formulations has been sporadically reported in the literature. Drakshadi (DK) and Gandharvahastadi (GK) decoctions are widely prescribed classical Ayurvedic herbal formulations. The potential adverse heavy metal toxicity due to consequent intake of DK and GK have been published in scientific papers or reported through pharmacovigilance. The objective of our investigation was to determine the presence of heavy metals in marketed DK and GK, with reference to the Ayurveda Pharmacopeia of India (API) standards. In this study, the concentration of four heavy metals, Arsenic (As), Cadmium (Cd), Lead (Pb), and Mercury (Hg) were investigated using Inductively Coupled Plasma- Mass Spectrometer (ICP-MS). These metals are not known to have any role in the pharmacological activity of these formulations. However, their presence beyond acceptable limits can have harmful consequences. Eighteen random samples each of DK and GK decoctions were collected from different pharmacy outlets, Kerala province, India. We tested 36 market samples of the above formulations for heavy metals, which were found to be within the limits prescribed by the Ayurvedic Pharmacopoeia of India. Our study suggests that heavy metal contamination of Ayurvedic formulations may not be widespread as suspected. However, continuous dynamic monitoring of Ayurvedic herbal formulations for heavy metal contamination is warranted considering the possibility of inadvertent contamination of raw drugs from environmental exposure. Page(s): 739-746 Thu, 01 Aug 2024 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/64437 2024-08-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/64436 Title: Pharmacognostic, phytochemical, and chromatographic fingerprinting of three probable species accepted as Kakoli – a member of Astavarga Authors: Achrya, Balkrishna; Mishra, Rajesh; Thakur, Pallavi; Singh, Shivam; Narsingh, Swami; Varshney, Anurag Abstract: Kakoli is an Ayurvedic drug, used for its effect on the reproductive system, especially for promoting spermatogenesis. According to Ayurvedic and other Sanskrit literature, three different plant species exist under the name of Kakoli, namely, Roscoea purpurea Sm., Roscoea capitata Sm., and Roscoea alpina Royle. Grouping multiple plant species under one name, often leads to the selection and inconsistent usage of non-authentic species as therapeutic drugs. In the present study, we compared the three Roscoea species, used as Kakoli, using pharmacognostical and phytochemical, as well as other analytical studies. We found that R. purpurea Sm. might be the most probable source of Kakoli as evidenced by the correlation obtained between literary resource-based parameters (ancient scriptures) and the experimental analysis (pharmacognostical and phytochemical findings) thereby establishing the authentic taxonomic identity of the valuable ancient medicine Kakoli. Page(s): 747-759 Thu, 01 Aug 2024 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/64436 2024-08-01T00:00:00Z