http://nopr.niscpr.res.in/handle/123456789/65688 Tue, 13 Oct 2026 01:16:06 GMT 2026-10-13T01:16:06Z http://nopr.niscpr.res.in/handle/123456789/65697 Title: Early-onset myocardial infarction may be related to ApoE and CYP2C19 variations Authors: Kirac, Deniz; Yaman, Aysun Erdem; Bayam, Emrah; Kucuk, Gizem Koprululu; Ozden, Kivilcım; Keles, Elif Cigdem Abstract: Myocardial infarction (MI) is among the leading causes of death in the world. While most MIs occur over the age of 65, 5-10% are detected in young individuals. This condition is known as early-onset MI. Since it is common in every population, there is a processing need of identifiying the causative factors of the disease. Genetic factors have an important effect for early-onset MI. Therefore, this study aimed to investigate the relationship between ApoE and CYP2C19 variations with early-onset MI. Thirty early-onset MI patients and 30 healthy individuals were included in the study. After DNA was isolated, CYP2C19 and ApoE variations were investigated by qPCR. Results were evaluated statistically. ε2 variant of ApoE and CYP2C19*2 were found statistically high in control and patient group, respectively. Hypertension, diabetes mellitus, smoking, triglyceride, LDL, total cholesterol, BMI and fasting blood glucose levels were statistically high in patient group. Some statistically significant associations were detected between hypertension, fasting blood glucose, LDL, HDL, BMI, total cholesterol and triglyceride levels with ε2; and between ε4 and CYP2C19*2 variants. CYP2C19*2 variant may have a strong association with early-onset MI and ε2 may have a protective role for the disease. It was considered that ε4 may affect the occurrence of the disease according to the increase in some blood parameters. In conclusion, screening of ApoE and CYP2C19 variations may provide information about early-onset MI. Page(s): 285-290 Tue, 01 Apr 2025 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/65697 2025-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/65696 Title: Subsarcolemmal mitochondrial dysfunction aggravates ischemia-reperfusion injury in diabetic rat hearts on fructose and cholesterol diets Authors: Ansari, Mahalakshmi; Prem, Priyanka N; Gino, Eren Rose; Kurian, Gino A Abstract: The rising prevalence of diabetes mellitus and metabolic syndrome, driven by diets high in fat and fructose, has significantly increased the risk of cardiovascular complications, including ischemia-reperfusion (IR) injury. Understanding the mechanisms linking dietary stressors to mitochondrial dysfunction in diabetes is crucial to addressing this health burden. Mitochondrial dysfunction, particularly in the subsarcolemmal fraction, is a hallmark feature of diabetic cardiomyopathy and IR injury. This study assessed the impact of a high-fat and fructose diet on IR injury in diabetic rat hearts. Forty-eight Wistar rats were assigned to four groups: normal, diabetes mellitus (DM), DM with a cholesterol diet (CD), and DM with a fructose diet (FD). Isolated hearts subjected to IR injury via Langendorff perfusion demonstrated cardiac hypertrophy, increased oxidative stress, and mitochondrial dysfunction in CD and FD groups compared to normal rats. FD rats exhibited significantly higher serum glucose, insulin resistance, brain natriuretic peptide expression, and subsarcolemmal mitochondrial damage, resulting in worse cardiac recovery and greater myocardial injury post-IR than CD rats. The findings underscore the detrimental effect of a fructose-rich diet on cardiac and mitochondrial health in diabetic conditions. This study highlights the need for dietary interventions targeting mitochondrial function to reduce cardiovascular risk in diabetic populations. Page(s): 291-300 Tue, 01 Apr 2025 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/65696 2025-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/65695 Title: Establishing PCOS in Wistar rats: A reliable model for understanding polycystic ovary syndrome Authors: Negi, Neha; Sharma, Indu Abstract: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder affecting reproductive-age women, characterised by metabolic and reproductive abnormalities. This study aimed to develop and evaluate a comprehensive rat model of PCOS that accurately replicates both the metabolic and reproductive facets of the syndrome. Female Wistar rats were treated with dehydroepiandrosterone (DHEA), high-fat diet (HFD), and a combination of both for 20 and 30 days. The study assessed body weight, estrous cyclicity, serum biochemistry, hormone levels, and ovarian histology. The DHEA+HFD combination model effectively mimicked PCOS characteristics and exhibited significant increases in body weight, disrupted estrous cycles, blood glucose, lipid levels, testosterone, estrogen, and LH levels, with decreased FSH levels. Liver and kidney function markers were also altered, indicating systemic effects. Further, histological examination of ovaries revealed cystlike follicles and reduced corpus luteum formation, resembling PCOS ovarian morphology. Moreover, DHEA alone induces reproductive changes without significant metabolic alterations, and HFD alone showed slow progression of metabolic features, but the combination group rapidly induced both metabolic and reproductive abnormalities within 20 to 30 days. The synergistic effect highlights the potential role of diet in exacerbating PCOS symptoms. Current study presents a rat model that comprehensively replicates PCOS features in a shorter timeframe. This combination model is valuable for investigating PCOS pathophysiology and potential therapeutic interventions. Furthermore, these findings underscore the importance of considering nutritional factors in PCOS management and open new avenues for research into the intricate relationship between PCOS-related metabolic and reproductive abnormalities. Page(s): 301-311 Tue, 01 Apr 2025 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/65695 2025-04-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/65694 Title: Anticancer effect of postbiotic derived from fermented milk of Lactobacillus helveticus MTCC 5463 on HT-29 Authors: Parmar, Urmila; V, Sreeja; S, Kiran; H S, Sandhya; Jakhesara, Subhash Abstract: There is increasing interest in postbiotics as potential therapeutic agents due to their biofunctional properties. As nonviable bacterial products or metabolites that offer health benefits, their anticancer potential warrants investigation. This study focuses on examining the effects of postbiotics derived from lactic acid bacteria fermented milk, specifically from Lactobacillus helveticus MTCC 5463, on colon cancer cells. This study screened postbiotics (cell-free supernatants) derived from the fermented milk of four LAB strains for various biofunctional activities. The most promising postbiotic, derived from Lactobacillus helveticus MTCC 5463 was then assessed for its anticancer effects on the HT-29 colon cancer cell line using the MTT assay. The study used the RT-PCR method to examine the impact of the postbiotic on the expression levels of genes associated with apoptosis, including Bax, Bcl-2, Caspase-8, cyclin D1, and p53. After 24, 48, and 72 h of treatment, the IC50 values of postbiotic were 3.0, 1.5, and 1.0 mg/mL, respectively. Gene expression analysis via RT-PCR revealed upregulation of pro-apoptotic genes (Bax and Caspase-8) and downregulation of antiapoptotic genes (Bcl-2, cyclin D1, and p53) in HT-29 cells treated with the postbiotic compared to untreated control cells. These findings suggest that the postbiotic derived from the fermented milk of probiotic MTCC 5463 could serve as a promising biological agent against colon cancer. Page(s): 312-322 Tue, 01 Apr 2025 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/65694 2025-04-01T00:00:00Z