http://nopr.niscpr.res.in/handle/123456789/68140 Thu, 08 Oct 2026 11:25:37 GMT 2026-10-08T11:25:37Z http://nopr.niscpr.res.in/handle/123456789/68179 Title: Phytopharmacological studies of botanical sources of sahachara: Barleria prionitis L. and Strobilanthes ciliate Nees Authors: N Gaidhani, Sudesh; B, Thirupataiah; R, Thulasi; G Nair, Parvathy; Misro, Lakshminarayana; Reddy S, Viswanth; Kumar Y R, Sanjay; Ala, Srikanth Abstract: Ayurveda, the traditional Indian system of medicine, extensively utilises medicinal plants; however, region-specific availability and increasing global demand have led to the substitution of botanically distinct species under the same vernacular names, often without scientific validation. Barleria prionitis L. and Strobilanthes ciliatus Nees, both identified as Sahachara in classical Ayurvedic literature, are frequently used interchangeably in clinical practice for the management of Vata-related disorders, including neuromuscular and inflammatory conditions, with S. ciliatus Nees frequently replacing B. prionitis L. The present study was undertaken to critically evaluate the validity of this substitution by comparing the phytochemical characteristics, quality control parameters, and pharmacological activities of both species. Aqueous and hydroalcoholic extracts of different plant parts were prepared using the cold maceration method. Phytochemical profiling was performed using High Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography–Mass Spectrometry (GC-MS/MS). Acute oral toxicity was assessed according to OECD guideline 423 using a limit dose of 2000 mg/kg. Analgesic and anti-inflammatory activities were evaluated in experimental animal models at doses of 200 and 400 mg/kg body weight. The study established key quality control parameters and revealed distinct chromatographic and GCMS/ MS profiles, identifying species-specific phytochemical markers. Both extracts were found to be safe, with LD₅₀ values exceeding 2000 mg/kg. In various analgesic models, aqueous and hydroalcoholic extracts of both plants produced significant analgesic effects compared to controls. A marked reduction in paw oedema volume confirmed their antiinflammatory activity; however, no significant alterations were observed in pro-inflammatory cytokines such as TNF-α and IL-6. Overall, the findings suggest comparable anti-inflammatory and analgesic potential of both species. Page(s): 215-237 Wed, 01 Jul 2026 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/68179 2026-07-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/68177 Title: Quantitative analysis of stigmasterol and α-Amyrin in three antidiabetic medicinal plants using GC-MS/MS Authors: Kumar, Dharmendra; S. Kolte, Satish Abstract: Traditional medicinal systems in India frequently employ Costus igneus N.E.Br., Bryophyllum pinnatum (Lam.) Oken, and Ficus benghalensis L. for the management of diabetes mellitus; however, systematic comparative quantitative evidence supporting their bioactive sterol content remains limited. The present investigation aimed to comparatively quantify two pharmacologically significant phytomarkers, stigmasterol and α-amyrin, in chloroform extracts of the leaves of C. igneus and B. pinnatum, and of the aerial roots of F. benghalensis, using a validated GC–MS/MS method operating in Multiple Reaction Monitoring (MRM) mode. Qualitative GC–MS profiling confirmed the presence of both analytes in all samples. Quantitative analysis revealed that C. igneus leaves contained the highest concentration of stigmasterol (4580±310 μg/kg), whereas F. benghalensis aerial roots were particularly rich in α-amyrin (3250±250 μg/kg). B. pinnatum exhibited moderate yet appreciable levels of both compounds. The distinct quantitative patterns observed suggest that although these plants share ethnomedicinal use in diabetes management, their chemical contributors may differ significantly. The findings establish a comparative phytochemical baseline that may guide future pharmacological validation and standardisation efforts. Page(s): 238-245 Wed, 01 Jul 2026 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/68177 2026-07-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/68175 Title: Bioconversion of D-limonene into value-added monoterpenes using Paenibacillus popilliae 1C and Streptomyces rochei AB1: Exploring bacterial biotransformation pathways and metabolite diversity Authors: Beichi, Madjeda; Saidani, Fatiha; Riad, Nacera; Elzahra Ferradji, Fatima; Elzahra Mesbaiah, Fatima; Reda Zahi, Mohamed; Eddouaouda, Kamel; Badis, Abdelmalek; El Hattab, Mohamed Abstract: The present study focuses on the bioconversion of D-limonene using two bacterial strains, Streptomyces rochei AB1 and Paenibacillus popilliae 1C, isolated and identified by our research team. Rich mediums (International Streptomyces Project 9 medium for strain AB1 and Luria–Bertani medium for strain 1C) were used for biotransformation experiments. Furthermore, biodegradation tests were conducted with D-limonene as the only carbon and energy source in a modified minimum medium. The minimum inhibitory concentration (MIC) of D-limonene was 10±0.09 mg/mL. The strains achieved a bioconversion yield of nearly 70% following 7-day of incubation period. Analysis by GC-MS revealed the formation of various hydrocarbons and oxygenated monoterpenes, including alcohols, ketones and terpene hydrocarbons. Some metabolites, such as cis-verbenol, isovaleric acid and 2-methylbutanoic acid, were identified in biotransformation assays using Paenibacillus popilliae 1C. To our knowledge, this genus has never before been known to produce these metabolites during the biotransformation of D-limonene. Carveol, carvone, limonene-1,2-diol, linalool, and α-terpinolene were among the other metabolites found. Similar biodegradation products were obtained with Streptomyces rochei AB1, suggesting the occurrence of common metabolic pathways involved in D-limonene bioconversion. These findings highlight the biotechnological potential of the studied strains for the production of value-added monoterpenes. Page(s): 246-255 Wed, 01 Jul 2026 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/68175 2026-07-01T00:00:00Z http://nopr.niscpr.res.in/handle/123456789/68172 Title: Extraction of T. cordifolia (Thunb.) by multiple solvents to investigate phytochemical recovery by LC-MS and greenness evaluation by AGREEprep and AGREE Authors: Dutta, Sreya; Kumar, Deepak; Mondal, Susmita; Kumar Meena, Ajay; Babu, Gajji; Hazra, Kalyan Abstract: Drug development from plant extracts using solvents of variable polarities has gained immense popularity. The selection of the solvent results in variable phytochemical profiles in the extracts. Solvent selection is primarily based on the targeted phytocompounds. The stem of Tinospora cordifolia is used to treat multiple ailments. In the present study, we have explored the extractability of organic solvents of varying polarity and evaluated the greenness of the analysis using various tools. The stem of T. cordifolia was extracted by traditional methods in accordance with the Pharmacopoeial guidelines, and phytochemical identification was performed by LC-MS. The methods of phytochemical identification were evaluated for their greenness indexation by using AGREEprep and AGREE tools. The experiment revealed that water was the best solvent for extraction (yield 14.25%) and that the LC-MS phytochemical profiles showed the highest number of phytochemicals (27). Water was the best option (AGREEprep score 0.80) for eco-friendly extraction. The study concludes that during drug development from plant extracts, we must adopt methods and solvents that support sustainable growth, which are expected to be perfectly eco-tuned. Page(s): 256-265 Wed, 01 Jul 2026 00:00:00 GMT http://nopr.niscpr.res.in/handle/123456789/68172 2026-07-01T00:00:00Z