Optimization of medium for lipase production by <i>Acinetobacter haemolyticus </i>from healthy human skin

Abstract

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A lipase producing <i style="">Acinetobacter haemolyticus</i> TA106 was isolated from healthy human skin of tribal population. The maximum activity of 55 U/ml was observed after medium optimization using the “one variable at a time” and the statistical approaches. The optimal composition of the medium was determined as (% w/v or v/v): tryptone - 1, yeast extract - 0.5, sodium chloride-1, olive oil-1, Tween - 80 1, manganese sulphate - 5 m<i style="">M</i>, sucrose- 1, <i style="">p</i>H-7. It was found that maximum production occurred in late log phase i.e. after 72 h and at 200 rpm. From factorial design and statistical analysis, it was found that <i style="">p</i>H, temperature, salt, inoculum density and aeration significantly affected the lipase production. It was also noted that inoculum density of 3 % (v/v), sucrose (1% w/v) and manganese sulphate(5 m<i style="">M</i>) displayed maximum lipase activity of 55 U/ml by conventional as well as statistical method. Optimization studies also indicated the increase in specific activity from 0.2 U/mg to 6.7 U/mg.</smarttagtype>